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Aquaporins as salivary protein biomarkers for xerostomia and the effects of xerostomia on oral health related quality of life in patients with periodontitis attending combined Military Hospital, Lahore

Xerostomia is a debilitating condition of oral dryness which may lead to numerous oral health conditions affecting oral health-related quality of life (OHRQOL). The general objectives of this study were to identify the potential of using salivary aquaporin (AQP)-3, AQP-4, and AQP-5 proteins as bi...

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Main Author: Atif, Saira
Format: Thesis
Language:English
Published: 2022
Subjects:
R Medicine
Online Access:http://eprints.usm.my/54631/1/SAIRA%20ATIF-FINAL%20THESIS%20P-SGD001117%28R%29%20PWD_-24%20pages.pdf
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spelling my.usm.eprints.54631 http://eprints.usm.my/54631/ Aquaporins as salivary protein biomarkers for xerostomia and the effects of xerostomia on oral health related quality of life in patients with periodontitis attending combined Military Hospital, Lahore Atif, Saira R Medicine Xerostomia is a debilitating condition of oral dryness which may lead to numerous oral health conditions affecting oral health-related quality of life (OHRQOL). The general objectives of this study were to identify the potential of using salivary aquaporin (AQP)-3, AQP-4, and AQP-5 proteins as biomarkers for xerostomia in patients with periodontitis and to investigate the effects of xerostomia on OHRQOL. In this descriptive study, 140 healthy participants, 20 – 55 years old, with Community Periodontal Index (CPI) score ≥ 2 were included through systematic random sampling. Shortened Xerostomia Inventory (SXI) was used for subjective assessment of xerostomia, while unstimulated saliva was collected for its objective measurement. Patient with SXI score ˃ 5 was considered xerostomic; score 6 – 8 as grade 1, and score 9 – 15 as grade 2 xerostomia. Shortened Oral Health Impact Profile (S-OHIP) questionnaire was utilised to assess OHRQOL. Quantification of AQP-3, AQP-4, and AQP-5 was done by enzyme-linked immunosorbent assay (ELISA). After removing extreme outliers, data of 132 participants were analysed. Probability value was set at 5%. Non-parametric tests were used for data analyses. Xerostomia was reported in 78% of the patients with periodontitis. Grade 1 xerostomia was reported in 74.8% whereas, grade 2 xerostomia in 25.2% of the periodontitis patients with xerostomia. OHRQOL was significantly poor in xerostomics compared to non xerostomics, p = .001, and in grade 2 compared to grade 1 xerostomia, p = .001. Unstimulated salivary flow rate was significantly lower in periodontitis patients with grade 1 compared to grade 2 xerostomia, p = .013. Concentration of AQP-3 was significantly higher in xerostomics compared to non-xerostomics, p < .001. Moreover, concentration of AQP-5 was significantly reduced in grade 2 compared to grade 1 xerostomia, p = .002. In the patients with periodontitis, there were significant correlations between SXI and S-OHIP (p < .001), SXI and AQP-3 (p = .004), AQP-3 and AQP-4 (p = .001), unstimulated salivary flow rate and AQP-4 (p = .035), and unstimulated salivary flow rate and AQP-5 (p = .007). SXI was a suitable predictor for poor OHRQOL. Moreover, S-OHIP score and AQP-3; and S-OHIP score and AQP-5 were suitable predictors of xerostomia and grade 2 xerostomia, respectively. In conclusion, salivary AQPs are suitable protein biomarkers for xerostomia in patients with periodontitis. It is important to manage xerostomia before it affects oral health by early detection using these biomarkers, as xerostomia is considerably prevalent and causes a deterioration of OHRQOL. 2022 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/54631/1/SAIRA%20ATIF-FINAL%20THESIS%20P-SGD001117%28R%29%20PWD_-24%20pages.pdf Atif, Saira (2022) Aquaporins as salivary protein biomarkers for xerostomia and the effects of xerostomia on oral health related quality of life in patients with periodontitis attending combined Military Hospital, Lahore. PhD thesis, Universiti Sains Malaysia.
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic R Medicine
spellingShingle R Medicine
Atif, Saira
Aquaporins as salivary protein biomarkers for xerostomia and the effects of xerostomia on oral health related quality of life in patients with periodontitis attending combined Military Hospital, Lahore
description Xerostomia is a debilitating condition of oral dryness which may lead to numerous oral health conditions affecting oral health-related quality of life (OHRQOL). The general objectives of this study were to identify the potential of using salivary aquaporin (AQP)-3, AQP-4, and AQP-5 proteins as biomarkers for xerostomia in patients with periodontitis and to investigate the effects of xerostomia on OHRQOL. In this descriptive study, 140 healthy participants, 20 – 55 years old, with Community Periodontal Index (CPI) score ≥ 2 were included through systematic random sampling. Shortened Xerostomia Inventory (SXI) was used for subjective assessment of xerostomia, while unstimulated saliva was collected for its objective measurement. Patient with SXI score ˃ 5 was considered xerostomic; score 6 – 8 as grade 1, and score 9 – 15 as grade 2 xerostomia. Shortened Oral Health Impact Profile (S-OHIP) questionnaire was utilised to assess OHRQOL. Quantification of AQP-3, AQP-4, and AQP-5 was done by enzyme-linked immunosorbent assay (ELISA). After removing extreme outliers, data of 132 participants were analysed. Probability value was set at 5%. Non-parametric tests were used for data analyses. Xerostomia was reported in 78% of the patients with periodontitis. Grade 1 xerostomia was reported in 74.8% whereas, grade 2 xerostomia in 25.2% of the periodontitis patients with xerostomia. OHRQOL was significantly poor in xerostomics compared to non xerostomics, p = .001, and in grade 2 compared to grade 1 xerostomia, p = .001. Unstimulated salivary flow rate was significantly lower in periodontitis patients with grade 1 compared to grade 2 xerostomia, p = .013. Concentration of AQP-3 was significantly higher in xerostomics compared to non-xerostomics, p < .001. Moreover, concentration of AQP-5 was significantly reduced in grade 2 compared to grade 1 xerostomia, p = .002. In the patients with periodontitis, there were significant correlations between SXI and S-OHIP (p < .001), SXI and AQP-3 (p = .004), AQP-3 and AQP-4 (p = .001), unstimulated salivary flow rate and AQP-4 (p = .035), and unstimulated salivary flow rate and AQP-5 (p = .007). SXI was a suitable predictor for poor OHRQOL. Moreover, S-OHIP score and AQP-3; and S-OHIP score and AQP-5 were suitable predictors of xerostomia and grade 2 xerostomia, respectively. In conclusion, salivary AQPs are suitable protein biomarkers for xerostomia in patients with periodontitis. It is important to manage xerostomia before it affects oral health by early detection using these biomarkers, as xerostomia is considerably prevalent and causes a deterioration of OHRQOL.
format Thesis
author Atif, Saira
author_facet Atif, Saira
author_sort Atif, Saira
title Aquaporins as salivary protein biomarkers for xerostomia and the effects of xerostomia on oral health related quality of life in patients with periodontitis attending combined Military Hospital, Lahore
title_short Aquaporins as salivary protein biomarkers for xerostomia and the effects of xerostomia on oral health related quality of life in patients with periodontitis attending combined Military Hospital, Lahore
title_full Aquaporins as salivary protein biomarkers for xerostomia and the effects of xerostomia on oral health related quality of life in patients with periodontitis attending combined Military Hospital, Lahore
title_fullStr Aquaporins as salivary protein biomarkers for xerostomia and the effects of xerostomia on oral health related quality of life in patients with periodontitis attending combined Military Hospital, Lahore
title_full_unstemmed Aquaporins as salivary protein biomarkers for xerostomia and the effects of xerostomia on oral health related quality of life in patients with periodontitis attending combined Military Hospital, Lahore
title_sort aquaporins as salivary protein biomarkers for xerostomia and the effects of xerostomia on oral health related quality of life in patients with periodontitis attending combined military hospital, lahore
publishDate 2022
url http://eprints.usm.my/54631/1/SAIRA%20ATIF-FINAL%20THESIS%20P-SGD001117%28R%29%20PWD_-24%20pages.pdf
http://eprints.usm.my/54631/
_version_ 1744354450294702080
score 13.211869

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