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Genotoxic evaluation of locally produced dental porcelain by using:comet assay

The aim of this study was to determine the genotoxicity of a locally produced dental porcelain (Universiti Sains Malaysia, Malaysia) using the Comet assay. The protocol for the Comet assay was followed as per the guidelines proposed by Tice eta!. (2000). L929 (CCL-1 A TCC, USA) mouse fibroblast c...

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Main Author: Kannan, T.P.
Format: Article
Language:English
Published: Pusat Pengajian Sains Pergigian, Universiti Sains Malaysia 2010
Subjects:
RK Dentistry
Online Access:http://eprints.usm.my/54101/1/Dr.%20T%20.P%20.Kannan-Eprints..pdf
http://eprints.usm.my/54101/
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spelling my.usm.eprints.54101 http://eprints.usm.my/54101/ Genotoxic evaluation of locally produced dental porcelain by using:comet assay Kannan, T.P. RK Dentistry The aim of this study was to determine the genotoxicity of a locally produced dental porcelain (Universiti Sains Malaysia, Malaysia) using the Comet assay. The protocol for the Comet assay was followed as per the guidelines proposed by Tice eta!. (2000). L929 (CCL-1 A TCC, USA) mouse fibroblast cells were treated with the dental porcelain at three different concentrations (50, 100 and 200mg/ml) along with concurrent negative (Eagle's Minimal Essential Medium) and positive (Zinc sulfate) controls. DNA strand breaks were evaluated based on the mean tail moment for 150 comets/sample. There was no significant difference in the tail moment between the negative control and the different concentrations of the dental porcelain tested, suggesting that the locally produced dental porcelain did not induce any DNA damage. Also, there was no dose-dependent relationship on the tail moment. Zinc sulfate, which was used as a positive control induced significant DNA damage. The results indicate that the locally produced dental porcelain is non-genotoxic under the present test conditions Pusat Pengajian Sains Pergigian, Universiti Sains Malaysia 2010 Article NonPeerReviewed application/pdf en http://eprints.usm.my/54101/1/Dr.%20T%20.P%20.Kannan-Eprints..pdf Kannan, T.P. (2010) Genotoxic evaluation of locally produced dental porcelain by using:comet assay. Genotoxic evaluation of locally produced dental porcelain by using: comet assay. (Submitted)
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic RK Dentistry
spellingShingle RK Dentistry
Kannan, T.P.
Genotoxic evaluation of locally produced dental porcelain by using:comet assay
description The aim of this study was to determine the genotoxicity of a locally produced dental porcelain (Universiti Sains Malaysia, Malaysia) using the Comet assay. The protocol for the Comet assay was followed as per the guidelines proposed by Tice eta!. (2000). L929 (CCL-1 A TCC, USA) mouse fibroblast cells were treated with the dental porcelain at three different concentrations (50, 100 and 200mg/ml) along with concurrent negative (Eagle's Minimal Essential Medium) and positive (Zinc sulfate) controls. DNA strand breaks were evaluated based on the mean tail moment for 150 comets/sample. There was no significant difference in the tail moment between the negative control and the different concentrations of the dental porcelain tested, suggesting that the locally produced dental porcelain did not induce any DNA damage. Also, there was no dose-dependent relationship on the tail moment. Zinc sulfate, which was used as a positive control induced significant DNA damage. The results indicate that the locally produced dental porcelain is non-genotoxic under the present test conditions
format Article
author Kannan, T.P.
author_facet Kannan, T.P.
author_sort Kannan, T.P.
title Genotoxic evaluation of locally produced dental porcelain by using:comet assay
title_short Genotoxic evaluation of locally produced dental porcelain by using:comet assay
title_full Genotoxic evaluation of locally produced dental porcelain by using:comet assay
title_fullStr Genotoxic evaluation of locally produced dental porcelain by using:comet assay
title_full_unstemmed Genotoxic evaluation of locally produced dental porcelain by using:comet assay
title_sort genotoxic evaluation of locally produced dental porcelain by using:comet assay
publisher Pusat Pengajian Sains Pergigian, Universiti Sains Malaysia
publishDate 2010
url http://eprints.usm.my/54101/1/Dr.%20T%20.P%20.Kannan-Eprints..pdf
http://eprints.usm.my/54101/
_version_ 1743107784986066944
score 13.211869

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