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On 2,6,6-Trimethylcyclohex-2-Ene-1,4-Dione Biotransformation Beyond The Stationary Phase Of Baker’s Yeast Type III

The reduction of ketoisophorone by the whole cell, Baker’s yeast cell type II has been researched recently. One of the major product of the biotransformation reduction of 2,6,6- trimethylcyclohex-2-ene-1,4-dione (ketoisophorone) is (4R,6R)-4-hydroxy-2,6,6-trimethylcyclohexanone or also named as (4R...

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Main Author: Remlee, Mohd Ridzwan
Format: Monograph
Language:English
Published: Universiti Sains Malaysia 2018
Subjects:
T Technology
TP Chemical Technology
Online Access:http://eprints.usm.my/53930/1/On%202%2C6%2C6-Trimethylcyclohex-2-Ene-1%2C4-Dione%20Biotransformation%20Beyond%20The%20Stationary%20Phase%20Of%20Baker%E2%80%99s%20Yeast%20Type%20III_Mohd%20Ridzwan%20Remlee_K4_2018.pdf
http://eprints.usm.my/53930/
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spelling my.usm.eprints.53930 http://eprints.usm.my/53930/ On 2,6,6-Trimethylcyclohex-2-Ene-1,4-Dione Biotransformation Beyond The Stationary Phase Of Baker’s Yeast Type III Remlee, Mohd Ridzwan T Technology TP Chemical Technology The reduction of ketoisophorone by the whole cell, Baker’s yeast cell type II has been researched recently. One of the major product of the biotransformation reduction of 2,6,6- trimethylcyclohex-2-ene-1,4-dione (ketoisophorone) is (4R,6R)-4-hydroxy-2,6,6-trimethylcyclohexanone or also named as (4R,6R)-actinol using Saccharomyces cerevisiae in the present of glucose as the carbon source. The biotransformation of ketoisophorone (KIP) forms levodione also named as 6R-dihydro-oxoisophorone (DOIP), which is intermediate before further reduction into actinol (ACT). This experiment only focussed on the death phase of S. cerevisiae fermentation where the substrate, ketoisophorone(KIP) was introduced and the time duration of 22h of death phase which is started from 38th hour until 60th of fermentation of yeast cells. The availability and stability of cofactor, nicotinamide adenine dinucleotide (NADH) or nicotinamide adenine dinucleotide phosphate (NADPH) by the presence of glucose through Kreb’s cycle process was essential in the biotransformation of ketoisophorone during that phase was determined by the reading of absorbance using UV-Vis spectrophotometer. This work was also investigate the amount of substrate and intermediate present during 22 h of reaction in order to determine the activity of the two types of ezymes within the yeast cells which is responsible for this biotransformation which is carbonyl reductase (CBR) and enoate reductase (ER) to produce a chiral alcohol of (4R,6R)-actinol. The result showed that the death phase produced the intermediate as well as product which is actinol with the aid of cofactor availability during the reaction of biotransformation. Universiti Sains Malaysia 2018-06-01 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/53930/1/On%202%2C6%2C6-Trimethylcyclohex-2-Ene-1%2C4-Dione%20Biotransformation%20Beyond%20The%20Stationary%20Phase%20Of%20Baker%E2%80%99s%20Yeast%20Type%20III_Mohd%20Ridzwan%20Remlee_K4_2018.pdf Remlee, Mohd Ridzwan (2018) On 2,6,6-Trimethylcyclohex-2-Ene-1,4-Dione Biotransformation Beyond The Stationary Phase Of Baker’s Yeast Type III. Project Report. Universiti Sains Malaysia, Pusat Pengajian Kejuruteraan Kimia. (Submitted)
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic T Technology
TP Chemical Technology
spellingShingle T Technology
TP Chemical Technology
Remlee, Mohd Ridzwan
On 2,6,6-Trimethylcyclohex-2-Ene-1,4-Dione Biotransformation Beyond The Stationary Phase Of Baker’s Yeast Type III
description The reduction of ketoisophorone by the whole cell, Baker’s yeast cell type II has been researched recently. One of the major product of the biotransformation reduction of 2,6,6- trimethylcyclohex-2-ene-1,4-dione (ketoisophorone) is (4R,6R)-4-hydroxy-2,6,6-trimethylcyclohexanone or also named as (4R,6R)-actinol using Saccharomyces cerevisiae in the present of glucose as the carbon source. The biotransformation of ketoisophorone (KIP) forms levodione also named as 6R-dihydro-oxoisophorone (DOIP), which is intermediate before further reduction into actinol (ACT). This experiment only focussed on the death phase of S. cerevisiae fermentation where the substrate, ketoisophorone(KIP) was introduced and the time duration of 22h of death phase which is started from 38th hour until 60th of fermentation of yeast cells. The availability and stability of cofactor, nicotinamide adenine dinucleotide (NADH) or nicotinamide adenine dinucleotide phosphate (NADPH) by the presence of glucose through Kreb’s cycle process was essential in the biotransformation of ketoisophorone during that phase was determined by the reading of absorbance using UV-Vis spectrophotometer. This work was also investigate the amount of substrate and intermediate present during 22 h of reaction in order to determine the activity of the two types of ezymes within the yeast cells which is responsible for this biotransformation which is carbonyl reductase (CBR) and enoate reductase (ER) to produce a chiral alcohol of (4R,6R)-actinol. The result showed that the death phase produced the intermediate as well as product which is actinol with the aid of cofactor availability during the reaction of biotransformation.
format Monograph
author Remlee, Mohd Ridzwan
author_facet Remlee, Mohd Ridzwan
author_sort Remlee, Mohd Ridzwan
title On 2,6,6-Trimethylcyclohex-2-Ene-1,4-Dione Biotransformation Beyond The Stationary Phase Of Baker’s Yeast Type III
title_short On 2,6,6-Trimethylcyclohex-2-Ene-1,4-Dione Biotransformation Beyond The Stationary Phase Of Baker’s Yeast Type III
title_full On 2,6,6-Trimethylcyclohex-2-Ene-1,4-Dione Biotransformation Beyond The Stationary Phase Of Baker’s Yeast Type III
title_fullStr On 2,6,6-Trimethylcyclohex-2-Ene-1,4-Dione Biotransformation Beyond The Stationary Phase Of Baker’s Yeast Type III
title_full_unstemmed On 2,6,6-Trimethylcyclohex-2-Ene-1,4-Dione Biotransformation Beyond The Stationary Phase Of Baker’s Yeast Type III
title_sort on 2,6,6-trimethylcyclohex-2-ene-1,4-dione biotransformation beyond the stationary phase of baker’s yeast type iii
publisher Universiti Sains Malaysia
publishDate 2018
url http://eprints.usm.my/53930/1/On%202%2C6%2C6-Trimethylcyclohex-2-Ene-1%2C4-Dione%20Biotransformation%20Beyond%20The%20Stationary%20Phase%20Of%20Baker%E2%80%99s%20Yeast%20Type%20III_Mohd%20Ridzwan%20Remlee_K4_2018.pdf
http://eprints.usm.my/53930/
_version_ 1740826596340662272
score 13.211869

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