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CB1 cannabinoid receptor in brain mesolimbic system of mitragynine-sensitised albina wastar rats as a candidate molecular terget in mitragyna speciosa (KETUM) addiction

Emerging new psychoactive substances impose public health implications worldwide, and include plants' phytochemicals such as Mitragynine (MG) from Mitragyna speciosa (or 'Ketum'). Studies have demonstrated the reciprocal interaction between endocannabinoid (ECB) system and opioid sy...

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Main Author: Mustapha, Muzaimi
Format: Monograph
Language:English
Published: Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia 2015
Subjects:
R Medicine
Online Access:http://eprints.usm.my/53465/1/DR.%20MUZAIMI%20MUSTAPHA-Eprints.pdf
http://eprints.usm.my/53465/
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spelling my.usm.eprints.53465 http://eprints.usm.my/53465/ CB1 cannabinoid receptor in brain mesolimbic system of mitragynine-sensitised albina wastar rats as a candidate molecular terget in mitragyna speciosa (KETUM) addiction Mustapha, Muzaimi R Medicine Emerging new psychoactive substances impose public health implications worldwide, and include plants' phytochemicals such as Mitragynine (MG) from Mitragyna speciosa (or 'Ketum'). Studies have demonstrated the reciprocal interaction between endocannabinoid (ECB) system and opioid systems in the modulation of brain-reward and neurobiological mechanisms underlying drug addiction. To date the neurochemical basis of Ketum abuse potential remains elusive. This research attempted to implicate ECB system in the abuse liabilities of emerging psychoactive plant, Ketum and its opioid-like alkaloid, mitragynine (MG). We had demonstrated the locomotor and behavioural effects of MG-sensitised Swiss albino mice in lntelliCage® using an approach of context-independent sensitisation. Animals were later sacrificed for immunohistochemical localisation of CB1 receptor in the brain with confirmatory analysis using Western blotting of the brain homogenates. Adult Albino Swiss mice were subjected to experimental groups of MG alone; MG + NIDA- 41020 (CB1 receptor antagonist); Morphine sulphate; .0.-9-Tetrahydrocannabinol + NIDA-41020; and vehicle. Daily intraperitoneal injection (i.p) of MG (5 to 25 mg/kg; 5mg/kg increment) at a 6-day interval was administered for 30 days, and plus once daily, 20mg NIDA-41 020 (oral). Findings to date showed neuroadaptive chronic exposure of morphine, and works are in its final phase for comparison with MG and in the presence of CB1 antagonist. Data analysis to date provide supportive ground for the first time to our knowledge, to indicate the involvement of CB1 receptor as the neural target of Ketum misuse potential. (Two manuscripts in preparation for potential publication in indexed-journals). Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia 2015 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/53465/1/DR.%20MUZAIMI%20MUSTAPHA-Eprints.pdf Mustapha, Muzaimi (2015) CB1 cannabinoid receptor in brain mesolimbic system of mitragynine-sensitised albina wastar rats as a candidate molecular terget in mitragyna speciosa (KETUM) addiction. Other. Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia. (Submitted)
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic R Medicine
spellingShingle R Medicine
Mustapha, Muzaimi
CB1 cannabinoid receptor in brain mesolimbic system of mitragynine-sensitised albina wastar rats as a candidate molecular terget in mitragyna speciosa (KETUM) addiction
description Emerging new psychoactive substances impose public health implications worldwide, and include plants' phytochemicals such as Mitragynine (MG) from Mitragyna speciosa (or 'Ketum'). Studies have demonstrated the reciprocal interaction between endocannabinoid (ECB) system and opioid systems in the modulation of brain-reward and neurobiological mechanisms underlying drug addiction. To date the neurochemical basis of Ketum abuse potential remains elusive. This research attempted to implicate ECB system in the abuse liabilities of emerging psychoactive plant, Ketum and its opioid-like alkaloid, mitragynine (MG). We had demonstrated the locomotor and behavioural effects of MG-sensitised Swiss albino mice in lntelliCage® using an approach of context-independent sensitisation. Animals were later sacrificed for immunohistochemical localisation of CB1 receptor in the brain with confirmatory analysis using Western blotting of the brain homogenates. Adult Albino Swiss mice were subjected to experimental groups of MG alone; MG + NIDA- 41020 (CB1 receptor antagonist); Morphine sulphate; .0.-9-Tetrahydrocannabinol + NIDA-41020; and vehicle. Daily intraperitoneal injection (i.p) of MG (5 to 25 mg/kg; 5mg/kg increment) at a 6-day interval was administered for 30 days, and plus once daily, 20mg NIDA-41 020 (oral). Findings to date showed neuroadaptive chronic exposure of morphine, and works are in its final phase for comparison with MG and in the presence of CB1 antagonist. Data analysis to date provide supportive ground for the first time to our knowledge, to indicate the involvement of CB1 receptor as the neural target of Ketum misuse potential. (Two manuscripts in preparation for potential publication in indexed-journals).
format Monograph
author Mustapha, Muzaimi
author_facet Mustapha, Muzaimi
author_sort Mustapha, Muzaimi
title CB1 cannabinoid receptor in brain mesolimbic system of mitragynine-sensitised albina wastar rats as a candidate molecular terget in mitragyna speciosa (KETUM) addiction
title_short CB1 cannabinoid receptor in brain mesolimbic system of mitragynine-sensitised albina wastar rats as a candidate molecular terget in mitragyna speciosa (KETUM) addiction
title_full CB1 cannabinoid receptor in brain mesolimbic system of mitragynine-sensitised albina wastar rats as a candidate molecular terget in mitragyna speciosa (KETUM) addiction
title_fullStr CB1 cannabinoid receptor in brain mesolimbic system of mitragynine-sensitised albina wastar rats as a candidate molecular terget in mitragyna speciosa (KETUM) addiction
title_full_unstemmed CB1 cannabinoid receptor in brain mesolimbic system of mitragynine-sensitised albina wastar rats as a candidate molecular terget in mitragyna speciosa (KETUM) addiction
title_sort cb1 cannabinoid receptor in brain mesolimbic system of mitragynine-sensitised albina wastar rats as a candidate molecular terget in mitragyna speciosa (ketum) addiction
publisher Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia
publishDate 2015
url http://eprints.usm.my/53465/1/DR.%20MUZAIMI%20MUSTAPHA-Eprints.pdf
http://eprints.usm.my/53465/
_version_ 1739828981640724480
score 13.211869

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