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The Effect Of Growing Cells And In-Situ Biotransformation Of 2,6,6-Trimethylcyclohex-2-Ene-1,4-Dione Towards Products Formation

Actinol (ACT) is the substrate needed for the synthesis of optically active xanthophylls, xanthoxin and zeaxanthins as well as aroma constituent of tobacco and saffron can be produced by the reduction of ketoisophorone (KIP) with high yield and enantioselectivity. The ACT can be produced from the bi...

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Main Author: Hari, Siva Sree
Format: Monograph
Language:English
Published: Universiti Sains Malaysia 2017
Subjects:
T Technology
TP155-156 Chemical engineering
Online Access:http://eprints.usm.my/53331/1/The%20Effect%20Of%20Growing%20Cells%20And%20In-Situ%20Biotransformation%20Of%202%2C6%2C6-Trimethylcyclohex-2-Ene-1%2C4-Dione%20Towards%20Products%20Formation_Siva%20Sree%20Hari%20Muniandy_K4_2017.pdf
http://eprints.usm.my/53331/
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spelling my.usm.eprints.53331 http://eprints.usm.my/53331/ The Effect Of Growing Cells And In-Situ Biotransformation Of 2,6,6-Trimethylcyclohex-2-Ene-1,4-Dione Towards Products Formation Hari, Siva Sree T Technology TP155-156 Chemical engineering Actinol (ACT) is the substrate needed for the synthesis of optically active xanthophylls, xanthoxin and zeaxanthins as well as aroma constituent of tobacco and saffron can be produced by the reduction of ketoisophorone (KIP) with high yield and enantioselectivity. The ACT can be produced from the biotransformation of KIP. The biotransformation of KIP forms levodione (DOIP), which is intermediate before further reduction into ACT. The biotransformation of KIP was studied during the growth phase of S. cerevisiae with the presence and absence of glucose. During the growth phase of S. cerevisiae, the biotransformation of KIP was only able to produce intermediate and the product was yet to be formed. The rate of biotransformation of KIP was faster with the presence of glucose. This was because presence of glucose increases the concentration of NADH and NADPH which was the essential cofactor needed for biotransformation of KIP. Thus, the rate of reduction of KIP was higher resulting in higher concentration of DOIP. When the rate of biotransformation was high, the production of ACT will also be faster. Therefore, the presence of glucose was important for the biotransformation of KIP. Universiti Sains Malaysia 2017-05-01 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/53331/1/The%20Effect%20Of%20Growing%20Cells%20And%20In-Situ%20Biotransformation%20Of%202%2C6%2C6-Trimethylcyclohex-2-Ene-1%2C4-Dione%20Towards%20Products%20Formation_Siva%20Sree%20Hari%20Muniandy_K4_2017.pdf Hari, Siva Sree (2017) The Effect Of Growing Cells And In-Situ Biotransformation Of 2,6,6-Trimethylcyclohex-2-Ene-1,4-Dione Towards Products Formation. Project Report. Universiti Sains Malaysia, Pusat Pengajian Kejuruteraan Kimia. (Submitted)
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic T Technology
TP155-156 Chemical engineering
spellingShingle T Technology
TP155-156 Chemical engineering
Hari, Siva Sree
The Effect Of Growing Cells And In-Situ Biotransformation Of 2,6,6-Trimethylcyclohex-2-Ene-1,4-Dione Towards Products Formation
description Actinol (ACT) is the substrate needed for the synthesis of optically active xanthophylls, xanthoxin and zeaxanthins as well as aroma constituent of tobacco and saffron can be produced by the reduction of ketoisophorone (KIP) with high yield and enantioselectivity. The ACT can be produced from the biotransformation of KIP. The biotransformation of KIP forms levodione (DOIP), which is intermediate before further reduction into ACT. The biotransformation of KIP was studied during the growth phase of S. cerevisiae with the presence and absence of glucose. During the growth phase of S. cerevisiae, the biotransformation of KIP was only able to produce intermediate and the product was yet to be formed. The rate of biotransformation of KIP was faster with the presence of glucose. This was because presence of glucose increases the concentration of NADH and NADPH which was the essential cofactor needed for biotransformation of KIP. Thus, the rate of reduction of KIP was higher resulting in higher concentration of DOIP. When the rate of biotransformation was high, the production of ACT will also be faster. Therefore, the presence of glucose was important for the biotransformation of KIP.
format Monograph
author Hari, Siva Sree
author_facet Hari, Siva Sree
author_sort Hari, Siva Sree
title The Effect Of Growing Cells And In-Situ Biotransformation Of 2,6,6-Trimethylcyclohex-2-Ene-1,4-Dione Towards Products Formation
title_short The Effect Of Growing Cells And In-Situ Biotransformation Of 2,6,6-Trimethylcyclohex-2-Ene-1,4-Dione Towards Products Formation
title_full The Effect Of Growing Cells And In-Situ Biotransformation Of 2,6,6-Trimethylcyclohex-2-Ene-1,4-Dione Towards Products Formation
title_fullStr The Effect Of Growing Cells And In-Situ Biotransformation Of 2,6,6-Trimethylcyclohex-2-Ene-1,4-Dione Towards Products Formation
title_full_unstemmed The Effect Of Growing Cells And In-Situ Biotransformation Of 2,6,6-Trimethylcyclohex-2-Ene-1,4-Dione Towards Products Formation
title_sort effect of growing cells and in-situ biotransformation of 2,6,6-trimethylcyclohex-2-ene-1,4-dione towards products formation
publisher Universiti Sains Malaysia
publishDate 2017
url http://eprints.usm.my/53331/1/The%20Effect%20Of%20Growing%20Cells%20And%20In-Situ%20Biotransformation%20Of%202%2C6%2C6-Trimethylcyclohex-2-Ene-1%2C4-Dione%20Towards%20Products%20Formation_Siva%20Sree%20Hari%20Muniandy_K4_2017.pdf
http://eprints.usm.my/53331/
_version_ 1738511189994897408
score 13.211869

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