Staff View: Molecular characterization of Selar crumeenophthabnus (ikan Lolong), Decapterus 111aruadsi (ikan Sadin) and Rastrelliger kanagurta (ikan kembong hitatn) fish species using mtDNA COI gene (DNA barcoding)

Molecular characterization of Selar crumeenophthabnus (ikan Lolong), Decapterus 111aruadsi (ikan Sadin) and Rastrelliger kanagurta (ikan kembong hitatn) fish species using mtDNA COI gene (DNA barcoding)

This study was undertaken to design a set of common primers to amplify the mitochondria DNA (mtDNA) cytochrome coxidase subunit I (COl) barcoding region of marine fish species. The primer set namely: forward primer (ST F I) "CAC AAA GAC ATC GGC ACCC" and the reverse primer (ST R3) "...

Full description

Saved in:

Bibliographic Details
Main Author:	Kee, Loo Suet
Format:	Monograph
Language:	English
Published:	Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia 2011
Subjects:	R Medicine
Online Access:	http://eprints.usm.my/52794/1/LOO%20SUET%20KEE-24%20pages.pdf http://eprints.usm.my/52794/
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my.usm.eprints.52794
record_format	eprints
spelling	my.usm.eprints.52794 http://eprints.usm.my/52794/ Molecular characterization of Selar crumeenophthabnus (ikan Lolong), Decapterus 111aruadsi (ikan Sadin) and Rastrelliger kanagurta (ikan kembong hitatn) fish species using mtDNA COI gene (DNA barcoding) Kee, Loo Suet R Medicine This study was undertaken to design a set of common primers to amplify the mitochondria DNA (mtDNA) cytochrome coxidase subunit I (COl) barcoding region of marine fish species. The primer set namely: forward primer (ST F I) "CAC AAA GAC ATC GGC ACCC" and the reverse primer (ST R3) "AAG AAT CANG AAT AG/AG TOT TG" were able to amplify the three species chosen for this study. The sequence data generated were compared and analysed using the sequence of mtDNA COl gene of Gobio gobio (GenBank accession no.: AB239596) as reference. On analysis, about 20% polymorphic positional variations were detected across the three species. The sequence data generated were used to study their phylogenetic relationship by constructing a rooted neighbor joining (NJ) dendrogram Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia 2011 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/52794/1/LOO%20SUET%20KEE-24%20pages.pdf Kee, Loo Suet (2011) Molecular characterization of Selar crumeenophthabnus (ikan Lolong), Decapterus 111aruadsi (ikan Sadin) and Rastrelliger kanagurta (ikan kembong hitatn) fish species using mtDNA COI gene (DNA barcoding). Other. Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia. (Submitted)
institution	Universiti Sains Malaysia
building	Hamzah Sendut Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Sains Malaysia
content_source	USM Institutional Repository
url_provider	http://eprints.usm.my/
language	English
topic	R Medicine
spellingShingle	R Medicine Kee, Loo Suet Molecular characterization of Selar crumeenophthabnus (ikan Lolong), Decapterus 111aruadsi (ikan Sadin) and Rastrelliger kanagurta (ikan kembong hitatn) fish species using mtDNA COI gene (DNA barcoding)
description	This study was undertaken to design a set of common primers to amplify the mitochondria DNA (mtDNA) cytochrome coxidase subunit I (COl) barcoding region of marine fish species. The primer set namely: forward primer (ST F I) "CAC AAA GAC ATC GGC ACCC" and the reverse primer (ST R3) "AAG AAT CANG AAT AG/AG TOT TG" were able to amplify the three species chosen for this study. The sequence data generated were compared and analysed using the sequence of mtDNA COl gene of Gobio gobio (GenBank accession no.: AB239596) as reference. On analysis, about 20% polymorphic positional variations were detected across the three species. The sequence data generated were used to study their phylogenetic relationship by constructing a rooted neighbor joining (NJ) dendrogram
format	Monograph
author	Kee, Loo Suet
author_facet	Kee, Loo Suet
author_sort	Kee, Loo Suet
title	Molecular characterization of Selar crumeenophthabnus (ikan Lolong), Decapterus 111aruadsi (ikan Sadin) and Rastrelliger kanagurta (ikan kembong hitatn) fish species using mtDNA COI gene (DNA barcoding)
title_short	Molecular characterization of Selar crumeenophthabnus (ikan Lolong), Decapterus 111aruadsi (ikan Sadin) and Rastrelliger kanagurta (ikan kembong hitatn) fish species using mtDNA COI gene (DNA barcoding)
title_full	Molecular characterization of Selar crumeenophthabnus (ikan Lolong), Decapterus 111aruadsi (ikan Sadin) and Rastrelliger kanagurta (ikan kembong hitatn) fish species using mtDNA COI gene (DNA barcoding)
title_fullStr	Molecular characterization of Selar crumeenophthabnus (ikan Lolong), Decapterus 111aruadsi (ikan Sadin) and Rastrelliger kanagurta (ikan kembong hitatn) fish species using mtDNA COI gene (DNA barcoding)
title_full_unstemmed	Molecular characterization of Selar crumeenophthabnus (ikan Lolong), Decapterus 111aruadsi (ikan Sadin) and Rastrelliger kanagurta (ikan kembong hitatn) fish species using mtDNA COI gene (DNA barcoding)
title_sort	molecular characterization of selar crumeenophthabnus (ikan lolong), decapterus 111aruadsi (ikan sadin) and rastrelliger kanagurta (ikan kembong hitatn) fish species using mtdna coi gene (dna barcoding)
publisher	Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia
publishDate	2011
url	http://eprints.usm.my/52794/1/LOO%20SUET%20KEE-24%20pages.pdf http://eprints.usm.my/52794/
_version_	1735387507689783296
score	13.211869

Molecular characterization of Selar crumeenophthabnus (ikan Lolong), Decapterus 111aruadsi (ikan Sadin) and Rastrelliger kanagurta (ikan kembong hitatn) fish species using mtDNA COI gene (DNA barcoding)

Similar Items