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Molecular characterization of scom_beromorus maculates (Ikan Tenggiri), atule Mate (Ikan Selar), and decapterus russelli (Ikan Selayang) species of fish from Malaysia marine ecosystem using dna sequence of mitochondrial dna (mtdna) cytochrome oxidase subunit I (COI) (DNA barcoding)

This study was undertaken to design a set of common primer to amplify the cytochrome oxidase I (COl) barcoding region of marine fish species. The primer set namely ST Fl 5'CACAAAGACATCGGCACCC3' (forward) and ST F3 S'AAGAATCAA/GAATAG/AGTGTTG3' (reverse) were able to amplify the...

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Main Author: Ismail, Che Alhafizol Che
Format: Monograph
Language:English
Published: Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia 2011
Subjects:
R Medicine
Online Access:http://eprints.usm.my/52652/1/CHE%20ALHAFIZOL%20BIN%20CHE%20ISMAIL-24%20pages.pdf
http://eprints.usm.my/52652/
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spelling my.usm.eprints.52652 http://eprints.usm.my/52652/ Molecular characterization of scom_beromorus maculates (Ikan Tenggiri), atule Mate (Ikan Selar), and decapterus russelli (Ikan Selayang) species of fish from Malaysia marine ecosystem using dna sequence of mitochondrial dna (mtdna) cytochrome oxidase subunit I (COI) (DNA barcoding) Ismail, Che Alhafizol Che R Medicine This study was undertaken to design a set of common primer to amplify the cytochrome oxidase I (COl) barcoding region of marine fish species. The primer set namely ST Fl 5'CACAAAGACATCGGCACCC3' (forward) and ST F3 S'AAGAATCAA/GAATAG/AGTGTTG3' (reverse) were able to amplify the three fish species namely Scomberomorus macu/ates (ikan Tenggiri), Atule mate (ikan Selar), and Decapterus russelli (ikan Selayang) chosen for this study. The sequence data generated were compared and analyzed using the sequence of COl gene of Gobio gobio (AB 239596) as a reference. On analysis between the three species Scomberomorus maculates and Atule mate have the highest percentage of similarities (76.91%), followed by Atule mate and Decapterus russelli (69.29%), and Scomberomorus maculates and Decapterus russe/li (64.45%) (Table 5). The sequence data generated were used to study their phylogenetic relationship by constructing a rooted neighbor joining (NJ) dendrogram (Fig. 18). Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia 2011 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/52652/1/CHE%20ALHAFIZOL%20BIN%20CHE%20ISMAIL-24%20pages.pdf Ismail, Che Alhafizol Che (2011) Molecular characterization of scom_beromorus maculates (Ikan Tenggiri), atule Mate (Ikan Selar), and decapterus russelli (Ikan Selayang) species of fish from Malaysia marine ecosystem using dna sequence of mitochondrial dna (mtdna) cytochrome oxidase subunit I (COI) (DNA barcoding). Other. Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia. (Submitted)
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic R Medicine
spellingShingle R Medicine
Ismail, Che Alhafizol Che
Molecular characterization of scom_beromorus maculates (Ikan Tenggiri), atule Mate (Ikan Selar), and decapterus russelli (Ikan Selayang) species of fish from Malaysia marine ecosystem using dna sequence of mitochondrial dna (mtdna) cytochrome oxidase subunit I (COI) (DNA barcoding)
description This study was undertaken to design a set of common primer to amplify the cytochrome oxidase I (COl) barcoding region of marine fish species. The primer set namely ST Fl 5'CACAAAGACATCGGCACCC3' (forward) and ST F3 S'AAGAATCAA/GAATAG/AGTGTTG3' (reverse) were able to amplify the three fish species namely Scomberomorus macu/ates (ikan Tenggiri), Atule mate (ikan Selar), and Decapterus russelli (ikan Selayang) chosen for this study. The sequence data generated were compared and analyzed using the sequence of COl gene of Gobio gobio (AB 239596) as a reference. On analysis between the three species Scomberomorus maculates and Atule mate have the highest percentage of similarities (76.91%), followed by Atule mate and Decapterus russelli (69.29%), and Scomberomorus maculates and Decapterus russe/li (64.45%) (Table 5). The sequence data generated were used to study their phylogenetic relationship by constructing a rooted neighbor joining (NJ) dendrogram (Fig. 18).
format Monograph
author Ismail, Che Alhafizol Che
author_facet Ismail, Che Alhafizol Che
author_sort Ismail, Che Alhafizol Che
title Molecular characterization of scom_beromorus maculates (Ikan Tenggiri), atule Mate (Ikan Selar), and decapterus russelli (Ikan Selayang) species of fish from Malaysia marine ecosystem using dna sequence of mitochondrial dna (mtdna) cytochrome oxidase subunit I (COI) (DNA barcoding)
title_short Molecular characterization of scom_beromorus maculates (Ikan Tenggiri), atule Mate (Ikan Selar), and decapterus russelli (Ikan Selayang) species of fish from Malaysia marine ecosystem using dna sequence of mitochondrial dna (mtdna) cytochrome oxidase subunit I (COI) (DNA barcoding)
title_full Molecular characterization of scom_beromorus maculates (Ikan Tenggiri), atule Mate (Ikan Selar), and decapterus russelli (Ikan Selayang) species of fish from Malaysia marine ecosystem using dna sequence of mitochondrial dna (mtdna) cytochrome oxidase subunit I (COI) (DNA barcoding)
title_fullStr Molecular characterization of scom_beromorus maculates (Ikan Tenggiri), atule Mate (Ikan Selar), and decapterus russelli (Ikan Selayang) species of fish from Malaysia marine ecosystem using dna sequence of mitochondrial dna (mtdna) cytochrome oxidase subunit I (COI) (DNA barcoding)
title_full_unstemmed Molecular characterization of scom_beromorus maculates (Ikan Tenggiri), atule Mate (Ikan Selar), and decapterus russelli (Ikan Selayang) species of fish from Malaysia marine ecosystem using dna sequence of mitochondrial dna (mtdna) cytochrome oxidase subunit I (COI) (DNA barcoding)
title_sort molecular characterization of scom_beromorus maculates (ikan tenggiri), atule mate (ikan selar), and decapterus russelli (ikan selayang) species of fish from malaysia marine ecosystem using dna sequence of mitochondrial dna (mtdna) cytochrome oxidase subunit i (coi) (dna barcoding)
publisher Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia
publishDate 2011
url http://eprints.usm.my/52652/1/CHE%20ALHAFIZOL%20BIN%20CHE%20ISMAIL-24%20pages.pdf
http://eprints.usm.my/52652/
_version_ 1734300773953568768
score 13.211869

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