Surface Functionalization And Characterization Of Antimicrobial Porous P(3hb-Co-4hb) Scaffold With Collagen Peptide For Biomedical Applications

P(3-hydroxybutyrate-co-4-hydroxybutyrate), [P(3HB-co-4HB)] is a bacterial derived biopolymer known for its high levels of biocompatability. However, surface of P(3HB-co-4HB) is found to be hydrophobic with minimal recognition sites for cell attachment. This makes it less desirable candidate to be ta...

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Main Author: Gurusamy, Tana Poorani
Format: Thesis
Language:English
Published: 2020
Subjects:
Online Access:http://eprints.usm.my/51671/1/TANA%20POORANI%20AP%20GURUSAMY%20-%20TESIS%20cut.pdf
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spelling my.usm.eprints.51671 http://eprints.usm.my/51671/ Surface Functionalization And Characterization Of Antimicrobial Porous P(3hb-Co-4hb) Scaffold With Collagen Peptide For Biomedical Applications Gurusamy, Tana Poorani QH1-278.5 Natural history (General) P(3-hydroxybutyrate-co-4-hydroxybutyrate), [P(3HB-co-4HB)] is a bacterial derived biopolymer known for its high levels of biocompatability. However, surface of P(3HB-co-4HB) is found to be hydrophobic with minimal recognition sites for cell attachment. This makes it less desirable candidate to be tailored as scaffolds for tissue engineering and biomedical applications. Due to this, this research was undertaken to fabricate P(3HB-co-4HB) with collagen peptides scaffolds using physiochemical modifications to further enhance their surface wettabilty as well as to support cell growth for biomedical applications. In this case, a bacterial strain, Cupriavidus malaysienesis sp. USMAA 1020 transformant, was found to synthesize P(3HB-co-4HB) from 4-hydroxybutyrate (4HB) precursor substrates, namely 1,4-butanediol and 1,6-hexanediol, respectatively. Firstly, a porous P(3HB-co-4HB) was fabricated through salt leaching and freeze drying techniques. This was then followed by the incorporation of collagen peptides at various concentrations (2.5 wt% - 12.5 wt%) to P(3HB-co-4HB) using two different modifications technique, amiolysis and collagen-coating. As a result, three types of P(3HB-co-4HB) scaffolds was fabricated including silver sulfadiazine(SSD) coated/collagen peptide-coated P(3HB-co-4HB) scaffolds, silver sulfadiazine blend/collagen peptide-coated P(3HB-co-4HB) scaffold and silver sulfadiazine blend/aminolysed P(3HB-co-4HB). 2020-05 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/51671/1/TANA%20POORANI%20AP%20GURUSAMY%20-%20TESIS%20cut.pdf Gurusamy, Tana Poorani (2020) Surface Functionalization And Characterization Of Antimicrobial Porous P(3hb-Co-4hb) Scaffold With Collagen Peptide For Biomedical Applications. Masters thesis, Universiti Sains Malaysia.
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic QH1-278.5 Natural history (General)
spellingShingle QH1-278.5 Natural history (General)
Gurusamy, Tana Poorani
Surface Functionalization And Characterization Of Antimicrobial Porous P(3hb-Co-4hb) Scaffold With Collagen Peptide For Biomedical Applications
description P(3-hydroxybutyrate-co-4-hydroxybutyrate), [P(3HB-co-4HB)] is a bacterial derived biopolymer known for its high levels of biocompatability. However, surface of P(3HB-co-4HB) is found to be hydrophobic with minimal recognition sites for cell attachment. This makes it less desirable candidate to be tailored as scaffolds for tissue engineering and biomedical applications. Due to this, this research was undertaken to fabricate P(3HB-co-4HB) with collagen peptides scaffolds using physiochemical modifications to further enhance their surface wettabilty as well as to support cell growth for biomedical applications. In this case, a bacterial strain, Cupriavidus malaysienesis sp. USMAA 1020 transformant, was found to synthesize P(3HB-co-4HB) from 4-hydroxybutyrate (4HB) precursor substrates, namely 1,4-butanediol and 1,6-hexanediol, respectatively. Firstly, a porous P(3HB-co-4HB) was fabricated through salt leaching and freeze drying techniques. This was then followed by the incorporation of collagen peptides at various concentrations (2.5 wt% - 12.5 wt%) to P(3HB-co-4HB) using two different modifications technique, amiolysis and collagen-coating. As a result, three types of P(3HB-co-4HB) scaffolds was fabricated including silver sulfadiazine(SSD) coated/collagen peptide-coated P(3HB-co-4HB) scaffolds, silver sulfadiazine blend/collagen peptide-coated P(3HB-co-4HB) scaffold and silver sulfadiazine blend/aminolysed P(3HB-co-4HB).
format Thesis
author Gurusamy, Tana Poorani
author_facet Gurusamy, Tana Poorani
author_sort Gurusamy, Tana Poorani
title Surface Functionalization And Characterization Of Antimicrobial Porous P(3hb-Co-4hb) Scaffold With Collagen Peptide For Biomedical Applications
title_short Surface Functionalization And Characterization Of Antimicrobial Porous P(3hb-Co-4hb) Scaffold With Collagen Peptide For Biomedical Applications
title_full Surface Functionalization And Characterization Of Antimicrobial Porous P(3hb-Co-4hb) Scaffold With Collagen Peptide For Biomedical Applications
title_fullStr Surface Functionalization And Characterization Of Antimicrobial Porous P(3hb-Co-4hb) Scaffold With Collagen Peptide For Biomedical Applications
title_full_unstemmed Surface Functionalization And Characterization Of Antimicrobial Porous P(3hb-Co-4hb) Scaffold With Collagen Peptide For Biomedical Applications
title_sort surface functionalization and characterization of antimicrobial porous p(3hb-co-4hb) scaffold with collagen peptide for biomedical applications
publishDate 2020
url http://eprints.usm.my/51671/1/TANA%20POORANI%20AP%20GURUSAMY%20-%20TESIS%20cut.pdf
http://eprints.usm.my/51671/
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score 13.211869