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A Comparative In-vitro Study of the Viability of Cultured Primary Normal Human Epidermal Keratinocytes on Cellular and Acellular Human Amniotic Membrane

Skin is the largest organ in human body. It plays important role to regulated and maintain human body homeostasis. Based on this condition, burned skin needs rehabilitation in order to reduce and avoid disturbances of body homeostasis. This study aimed to assess the ability of Human Amniotic Memb...

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Main Author: Mohd Yusri, Ahmad Fozi
Format: Monograph
Language:English
Published: Universiti Sains Malaysia 2009
Subjects:
R Medicine (General)
Online Access:http://eprints.usm.my/51216/1/MOHD%20YUSRI%20BIN%20AHMAD%20FOZI%20-%2024%20pages.pdf
http://eprints.usm.my/51216/
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spelling my.usm.eprints.51216 http://eprints.usm.my/51216/ A Comparative In-vitro Study of the Viability of Cultured Primary Normal Human Epidermal Keratinocytes on Cellular and Acellular Human Amniotic Membrane Mohd Yusri, Ahmad Fozi R Medicine (General) Skin is the largest organ in human body. It plays important role to regulated and maintain human body homeostasis. Based on this condition, burned skin needs rehabilitation in order to reduce and avoid disturbances of body homeostasis. This study aimed to assess the ability of Human Amniotic Membrane (HAM) as scaffold for the growth of keratinocytes in-vitro. The HAM's epithelium had been removed using enzymatic technique namely dispase and trypsin-EDTA. De-epithialized HAM (also known as an acellular of HAM) was used as the main scaffold for the cultured primary normal human epidermal keratinocytes (pNHEK). Cell viability was compared between deepithialized HAM and HAM without de-epithialization (also known as cellular HAM) for the three consequence days. Epithelium of HAM was successfully removed via dispase and trypsin-EDT A, as confirmed by histochemical and cytochemical analysis. De-epitheliazed HAM sustained better cell viability only at the first 24 hours compared with the HAM without de-epithialization. However, viable cells were deserved only at 48 hours postseeding of pNHEK on the HAM without de-epithialization. In conclusion, HAM can be deepithialized using enzymatic technique. De-epithialized HAM and HAM without deepithialization can sustain the growth of pNHEK. Result of this study will use to produces specific kind of HAM for healing of skin burn. Universiti Sains Malaysia 2009 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/51216/1/MOHD%20YUSRI%20BIN%20AHMAD%20FOZI%20-%2024%20pages.pdf Mohd Yusri, Ahmad Fozi (2009) A Comparative In-vitro Study of the Viability of Cultured Primary Normal Human Epidermal Keratinocytes on Cellular and Acellular Human Amniotic Membrane. Other. Universiti Sains Malaysia. (Submitted)
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic R Medicine (General)
spellingShingle R Medicine (General)
Mohd Yusri, Ahmad Fozi
A Comparative In-vitro Study of the Viability of Cultured Primary Normal Human Epidermal Keratinocytes on Cellular and Acellular Human Amniotic Membrane
description Skin is the largest organ in human body. It plays important role to regulated and maintain human body homeostasis. Based on this condition, burned skin needs rehabilitation in order to reduce and avoid disturbances of body homeostasis. This study aimed to assess the ability of Human Amniotic Membrane (HAM) as scaffold for the growth of keratinocytes in-vitro. The HAM's epithelium had been removed using enzymatic technique namely dispase and trypsin-EDTA. De-epithialized HAM (also known as an acellular of HAM) was used as the main scaffold for the cultured primary normal human epidermal keratinocytes (pNHEK). Cell viability was compared between deepithialized HAM and HAM without de-epithialization (also known as cellular HAM) for the three consequence days. Epithelium of HAM was successfully removed via dispase and trypsin-EDT A, as confirmed by histochemical and cytochemical analysis. De-epitheliazed HAM sustained better cell viability only at the first 24 hours compared with the HAM without de-epithialization. However, viable cells were deserved only at 48 hours postseeding of pNHEK on the HAM without de-epithialization. In conclusion, HAM can be deepithialized using enzymatic technique. De-epithialized HAM and HAM without deepithialization can sustain the growth of pNHEK. Result of this study will use to produces specific kind of HAM for healing of skin burn.
format Monograph
author Mohd Yusri, Ahmad Fozi
author_facet Mohd Yusri, Ahmad Fozi
author_sort Mohd Yusri, Ahmad Fozi
title A Comparative In-vitro Study of the Viability of Cultured Primary Normal Human Epidermal Keratinocytes on Cellular and Acellular Human Amniotic Membrane
title_short A Comparative In-vitro Study of the Viability of Cultured Primary Normal Human Epidermal Keratinocytes on Cellular and Acellular Human Amniotic Membrane
title_full A Comparative In-vitro Study of the Viability of Cultured Primary Normal Human Epidermal Keratinocytes on Cellular and Acellular Human Amniotic Membrane
title_fullStr A Comparative In-vitro Study of the Viability of Cultured Primary Normal Human Epidermal Keratinocytes on Cellular and Acellular Human Amniotic Membrane
title_full_unstemmed A Comparative In-vitro Study of the Viability of Cultured Primary Normal Human Epidermal Keratinocytes on Cellular and Acellular Human Amniotic Membrane
title_sort comparative in-vitro study of the viability of cultured primary normal human epidermal keratinocytes on cellular and acellular human amniotic membrane
publisher Universiti Sains Malaysia
publishDate 2009
url http://eprints.usm.my/51216/1/MOHD%20YUSRI%20BIN%20AHMAD%20FOZI%20-%2024%20pages.pdf
http://eprints.usm.my/51216/
_version_ 1724608794721255424
score 13.211869

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