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The investigation of cyclin-di gene expression using sybr green 1 based real-time pcr in bladder cancer

Bladder cancer is one of the most common malignancies in developed countries while transitional cell carcinomas (TCC) are the origin of more than 90% of diagnosed bladder cancers. Patients diagnosed with bladder cancer basically belong to two clinically distinct groups, namely non-muscle invasive...

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Main Author: Aisya, Zulkifli
Format: Monograph
Language:English
Published: Universiti Sains Malaysia 2009
Subjects:
R Medicine (General)
Online Access:http://eprints.usm.my/51027/1/AISYA%20BINTI%20ZULKIFLI%20-%2024%20pages.pdf
http://eprints.usm.my/51027/
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spelling my.usm.eprints.51027 http://eprints.usm.my/51027/ The investigation of cyclin-di gene expression using sybr green 1 based real-time pcr in bladder cancer Aisya, Zulkifli R Medicine (General) Bladder cancer is one of the most common malignancies in developed countries while transitional cell carcinomas (TCC) are the origin of more than 90% of diagnosed bladder cancers. Patients diagnosed with bladder cancer basically belong to two clinically distinct groups, namely non-muscle invasive (which are papillary pattern) and muscle invasive (which are solid). These carcinomas pose the greatest clinical problems due to the high recurrence of non-muscle invasive tumors even after transurethral resection of the tumors. At present, there are no clinically useful markers available for identifying bladder cancer patients with a high risk of disease recurrence or progression. Initial aim of this study was to investigate by using gene expression level of Cyclin-01. The experiment was carried out by using SBYR Green 1 based Real-Time PCR analysis. However, the result was not achieved as expected. This is due to some undetermined and invalid result few factors such as contamination, mispairing of the primer and primer dimer. Therefore the investigation of the factor that contributes to invalidation was investigated by using previous journals. Universiti Sains Malaysia 2009-10 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/51027/1/AISYA%20BINTI%20ZULKIFLI%20-%2024%20pages.pdf Aisya, Zulkifli (2009) The investigation of cyclin-di gene expression using sybr green 1 based real-time pcr in bladder cancer. Other. Universiti Sains Malaysia.
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic R Medicine (General)
spellingShingle R Medicine (General)
Aisya, Zulkifli
The investigation of cyclin-di gene expression using sybr green 1 based real-time pcr in bladder cancer
description Bladder cancer is one of the most common malignancies in developed countries while transitional cell carcinomas (TCC) are the origin of more than 90% of diagnosed bladder cancers. Patients diagnosed with bladder cancer basically belong to two clinically distinct groups, namely non-muscle invasive (which are papillary pattern) and muscle invasive (which are solid). These carcinomas pose the greatest clinical problems due to the high recurrence of non-muscle invasive tumors even after transurethral resection of the tumors. At present, there are no clinically useful markers available for identifying bladder cancer patients with a high risk of disease recurrence or progression. Initial aim of this study was to investigate by using gene expression level of Cyclin-01. The experiment was carried out by using SBYR Green 1 based Real-Time PCR analysis. However, the result was not achieved as expected. This is due to some undetermined and invalid result few factors such as contamination, mispairing of the primer and primer dimer. Therefore the investigation of the factor that contributes to invalidation was investigated by using previous journals.
format Monograph
author Aisya, Zulkifli
author_facet Aisya, Zulkifli
author_sort Aisya, Zulkifli
title The investigation of cyclin-di gene expression using sybr green 1 based real-time pcr in bladder cancer
title_short The investigation of cyclin-di gene expression using sybr green 1 based real-time pcr in bladder cancer
title_full The investigation of cyclin-di gene expression using sybr green 1 based real-time pcr in bladder cancer
title_fullStr The investigation of cyclin-di gene expression using sybr green 1 based real-time pcr in bladder cancer
title_full_unstemmed The investigation of cyclin-di gene expression using sybr green 1 based real-time pcr in bladder cancer
title_sort investigation of cyclin-di gene expression using sybr green 1 based real-time pcr in bladder cancer
publisher Universiti Sains Malaysia
publishDate 2009
url http://eprints.usm.my/51027/1/AISYA%20BINTI%20ZULKIFLI%20-%2024%20pages.pdf
http://eprints.usm.my/51027/
_version_ 1720980901798608896
score 13.211869

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