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Sequence polymorphism of mitochondrial DNA HVS II region of Malay population of peninsular Malaysia

Sequence variation in hypervariable II region (HVS-II) in the control region of mitochondrial DNA (mtDNA) was studied in 45 unrelated Malay individuals by PCR amplification and direct sequencing. A 267 bp fragment of HVS-II region was amplified using a set of primer HVC1 and HVDI. Agarose gel ele...

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Main Author: Wahab, Mohd Faizul Abd
Format: Monograph
Language:English
Published: Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia 2008
Subjects:
R Medicine
Online Access:http://eprints.usm.my/49748/1/MOHD%20FAIZUL%20BIN%20ABD%20WAHAB-24%20pages.pdf
http://eprints.usm.my/49748/
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spelling my.usm.eprints.49748 http://eprints.usm.my/49748/ Sequence polymorphism of mitochondrial DNA HVS II region of Malay population of peninsular Malaysia Wahab, Mohd Faizul Abd R Medicine Sequence variation in hypervariable II region (HVS-II) in the control region of mitochondrial DNA (mtDNA) was studied in 45 unrelated Malay individuals by PCR amplification and direct sequencing. A 267 bp fragment of HVS-II region was amplified using a set of primer HVC1 and HVDI. Agarose gel electrophoresis was carried to examine the polymerase chain reaction product. The product was purified prior to sequencing using QIAquick PCR Purification kit (QIAGEN). A total of 45 samples were sent for sequencing, but only 40 samples were successfully sequenced. Within the 40 sequences, 39 different haplotypes were observed, which 2 individuals shared the same haplotypes. From the result obtained, 42.5% of the samples were classified under haplogroup M, 12.5% were classified under haplogroup B, 10% were classified under haplogroup F and another 5% were classified under other haplogroup, D. A total27.5% of the samples were not yet classified. Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia 2008 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/49748/1/MOHD%20FAIZUL%20BIN%20ABD%20WAHAB-24%20pages.pdf Wahab, Mohd Faizul Abd (2008) Sequence polymorphism of mitochondrial DNA HVS II region of Malay population of peninsular Malaysia. Other. Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia. (Submitted)
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic R Medicine
spellingShingle R Medicine
Wahab, Mohd Faizul Abd
Sequence polymorphism of mitochondrial DNA HVS II region of Malay population of peninsular Malaysia
description Sequence variation in hypervariable II region (HVS-II) in the control region of mitochondrial DNA (mtDNA) was studied in 45 unrelated Malay individuals by PCR amplification and direct sequencing. A 267 bp fragment of HVS-II region was amplified using a set of primer HVC1 and HVDI. Agarose gel electrophoresis was carried to examine the polymerase chain reaction product. The product was purified prior to sequencing using QIAquick PCR Purification kit (QIAGEN). A total of 45 samples were sent for sequencing, but only 40 samples were successfully sequenced. Within the 40 sequences, 39 different haplotypes were observed, which 2 individuals shared the same haplotypes. From the result obtained, 42.5% of the samples were classified under haplogroup M, 12.5% were classified under haplogroup B, 10% were classified under haplogroup F and another 5% were classified under other haplogroup, D. A total27.5% of the samples were not yet classified.
format Monograph
author Wahab, Mohd Faizul Abd
author_facet Wahab, Mohd Faizul Abd
author_sort Wahab, Mohd Faizul Abd
title Sequence polymorphism of mitochondrial DNA HVS II region of Malay population of peninsular Malaysia
title_short Sequence polymorphism of mitochondrial DNA HVS II region of Malay population of peninsular Malaysia
title_full Sequence polymorphism of mitochondrial DNA HVS II region of Malay population of peninsular Malaysia
title_fullStr Sequence polymorphism of mitochondrial DNA HVS II region of Malay population of peninsular Malaysia
title_full_unstemmed Sequence polymorphism of mitochondrial DNA HVS II region of Malay population of peninsular Malaysia
title_sort sequence polymorphism of mitochondrial dna hvs ii region of malay population of peninsular malaysia
publisher Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia
publishDate 2008
url http://eprints.usm.my/49748/1/MOHD%20FAIZUL%20BIN%20ABD%20WAHAB-24%20pages.pdf
http://eprints.usm.my/49748/
_version_ 1709668329354952704
score 13.211869

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