Cover Image

Multifunctional Gold-Silica Composite Platform Towards Comprehensive Cancer Therapy

Incomplete cancer cells eradication with sole treatment of chemotherapy or radiation therapy was commonly reported. Therefore, gold nanoshells-based nanodevices have gained interests owing to the tuneablity of the localised surface plasmon resonance (LSPR) from visible to near infrared (NIR) wavelen...

Full description

Saved in:
Bibliographic Details
Main Author: Tew, Lih Shin
Format: Thesis
Language:English
Published: 2018
Subjects:
R Medicine
RC254-282 Neoplasms. Tumors. Oncology (including Cancer)
Online Access:http://eprints.usm.my/49710/1/TEW%20LIH%20SHIN_hj.pdf
http://eprints.usm.my/49710/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.usm.eprints.49710
record_format eprints
spelling my.usm.eprints.49710 http://eprints.usm.my/49710/ Multifunctional Gold-Silica Composite Platform Towards Comprehensive Cancer Therapy Tew, Lih Shin R Medicine RC254-282 Neoplasms. Tumors. Oncology (including Cancer) Incomplete cancer cells eradication with sole treatment of chemotherapy or radiation therapy was commonly reported. Therefore, gold nanoshells-based nanodevices have gained interests owing to the tuneablity of the localised surface plasmon resonance (LSPR) from visible to near infrared (NIR) wavelength, which is favourable for biomedical applications. In this work, gold nanoshell (GNS), of which has mesoporous silica nanoparticle core (MSN) filled doxorubicin (DOX) as well as polyethylene glycol (PEG) and AS1141 aptamer (Apt) at the outer layer (DOX-GNS-PEG-Apt), was designed for two photon laser-triggered chemo- and photothermal therapy. The results show 30.00 2.91 % of doxorubicin (DOX) was loaded into MSNs. The synthesised GNS was thenanalysed by UV-Vis spectroscopy and TEM, of which 4:1 ratio of K-gold to MSN-NH2 seed produced ideal gold thickness. The nanoparticles with a complete gold coverage on mesoporous silica core were observed. Based on the TEM and Energy Dispersive X-Ray spectroscopy (EDX) data, the gold film synthesised via grow-mediated process was approximately 10 – 15 nm thickness. Then, two photon excitation (TPE) was introduced to evaluate the efficacy of photothermal therapy (PTT) alone and the synergistic chemo-photothermal therapy. Deformation of GNS was evidenced by the blue shift of plasmon resonance peak from 800 to 570 nm and the morphology of the GNS after TPE was further confirmed with TEM. According to the in vitro study, DOX-GNS-PEG-Apt positive selectively bound to MDA-MB-231 cells (cancerous) but not MCF-10A cells (non-cancerous). 2018-08 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/49710/1/TEW%20LIH%20SHIN_hj.pdf Tew, Lih Shin (2018) Multifunctional Gold-Silica Composite Platform Towards Comprehensive Cancer Therapy. Masters thesis, Universiti Sains Malaysia.
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic R Medicine
RC254-282 Neoplasms. Tumors. Oncology (including Cancer)
spellingShingle R Medicine
RC254-282 Neoplasms. Tumors. Oncology (including Cancer)
Tew, Lih Shin
Multifunctional Gold-Silica Composite Platform Towards Comprehensive Cancer Therapy
description Incomplete cancer cells eradication with sole treatment of chemotherapy or radiation therapy was commonly reported. Therefore, gold nanoshells-based nanodevices have gained interests owing to the tuneablity of the localised surface plasmon resonance (LSPR) from visible to near infrared (NIR) wavelength, which is favourable for biomedical applications. In this work, gold nanoshell (GNS), of which has mesoporous silica nanoparticle core (MSN) filled doxorubicin (DOX) as well as polyethylene glycol (PEG) and AS1141 aptamer (Apt) at the outer layer (DOX-GNS-PEG-Apt), was designed for two photon laser-triggered chemo- and photothermal therapy. The results show 30.00 2.91 % of doxorubicin (DOX) was loaded into MSNs. The synthesised GNS was thenanalysed by UV-Vis spectroscopy and TEM, of which 4:1 ratio of K-gold to MSN-NH2 seed produced ideal gold thickness. The nanoparticles with a complete gold coverage on mesoporous silica core were observed. Based on the TEM and Energy Dispersive X-Ray spectroscopy (EDX) data, the gold film synthesised via grow-mediated process was approximately 10 – 15 nm thickness. Then, two photon excitation (TPE) was introduced to evaluate the efficacy of photothermal therapy (PTT) alone and the synergistic chemo-photothermal therapy. Deformation of GNS was evidenced by the blue shift of plasmon resonance peak from 800 to 570 nm and the morphology of the GNS after TPE was further confirmed with TEM. According to the in vitro study, DOX-GNS-PEG-Apt positive selectively bound to MDA-MB-231 cells (cancerous) but not MCF-10A cells (non-cancerous).
format Thesis
author Tew, Lih Shin
author_facet Tew, Lih Shin
author_sort Tew, Lih Shin
title Multifunctional Gold-Silica Composite Platform Towards Comprehensive Cancer Therapy
title_short Multifunctional Gold-Silica Composite Platform Towards Comprehensive Cancer Therapy
title_full Multifunctional Gold-Silica Composite Platform Towards Comprehensive Cancer Therapy
title_fullStr Multifunctional Gold-Silica Composite Platform Towards Comprehensive Cancer Therapy
title_full_unstemmed Multifunctional Gold-Silica Composite Platform Towards Comprehensive Cancer Therapy
title_sort multifunctional gold-silica composite platform towards comprehensive cancer therapy
publishDate 2018
url http://eprints.usm.my/49710/1/TEW%20LIH%20SHIN_hj.pdf
http://eprints.usm.my/49710/
_version_ 1709668323868803072
score 13.211869

Similar Items