Production Of Novel Recombinant Antipfhrp2 Vnar-G1 Protein Using Escherichia Coli Bl21(De3) Expression System
Malaria rapid diagnostic tests (RDTs) act as important antibody-based immunoassays for prompt malaria diagnosis. Conventional monoclonal antibodies (mAbs) are widely used in RDTs but it can be easily degraded at high ambient temperatures. Hence, the shark VNARS might be good alternatives to mAbs du...
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| Main Author: | |
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| Format: | Monograph |
| Language: | English |
| Published: |
Universiti Sains Malaysia
2020
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| Subjects: | |
| Online Access: | http://eprints.usm.my/49503/1/Kok%20Boon%20Hui.pdf%20cut.pdf http://eprints.usm.my/49503/ |
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| Summary: | Malaria rapid diagnostic tests (RDTs) act as important antibody-based immunoassays for prompt malaria diagnosis. Conventional monoclonal antibodies (mAbs) are
widely used in RDTs but it can be easily degraded at high ambient temperatures. Hence, the shark VNARS might be good alternatives to mAbs due to its higher thermal
stability and binding affinity with antigens. In this study, the recombinant anti- PfHRP2 VNAR-G1 protein was produced in E. coli expression system through various
steps such as recombinant cell isolation, PCR, agarose gel electrophoresis, plasmid extraction, transformation and protein expression. Besides, the combinatorial effects of temperature and IPTG concentration towards the cell density of recombinant BL21(DE3) based on the absorbance readings and cell wet weights were investigated using software R. |
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