Cover Image

Cloning and expression of ga protein of nip ah virus in yeast expression system

Nipah virus (NiV) is a deadly zoonotic paramyxovirus that has emerged and reemerged over the last 10 years. In human, the infection leads to encephalitis with 92% mortality rate. This has underlined the importance for the development of a vaccine candidate in preventing such zoonotic disease. In...

Full description

Saved in:
Bibliographic Details
Main Author: Chang Ching, Ching
Format: Monograph
Language:English
Published: Universiti Sains Malaysia 2008
Subjects:
R Medicine (General)
Online Access:http://eprints.usm.my/48915/1/CHANG%20CHING%20CHING%20-%2024%20pages.pdf
http://eprints.usm.my/48915/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.usm.eprints.48915
record_format eprints
spelling my.usm.eprints.48915 http://eprints.usm.my/48915/ Cloning and expression of ga protein of nip ah virus in yeast expression system Chang Ching, Ching R Medicine (General) Nipah virus (NiV) is a deadly zoonotic paramyxovirus that has emerged and reemerged over the last 10 years. In human, the infection leads to encephalitis with 92% mortality rate. This has underlined the importance for the development of a vaccine candidate in preventing such zoonotic disease. In previous study, the gene encoding the NiV glycoprotein (NiV -G) was cloned into the yeast Pichia Pastoris expression vector (pZMF) under the control of AOXI promoter. However, the recombinant G glycoprotein expression was found to be highly unstable in this system. Therefore, the stability of the recombinant protein was investigated by studying one of the truncated gene fragment (GA gene). The truncated gene encoding the gene sequence 1-867 was cloned and expressed in the same biological system. The detection of the truncated recombinant protein was performed through Western blot analysis. The result of this study indicated that the truncated fragment was stable and the expression was consistent with protein molecular weight of 40 kDa. The absent of degradation incident in this study had suggested that GA gene fragment was stable in full length G-glycoprotein expression. In addition, the truncated recombinant protein could be utilized as a promising vaccine candidate if the recombinant protein was found to be highly antigenic and immunogenic. Universiti Sains Malaysia 2008 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/48915/1/CHANG%20CHING%20CHING%20-%2024%20pages.pdf Chang Ching, Ching (2008) Cloning and expression of ga protein of nip ah virus in yeast expression system. Other. Universiti Sains Malaysia. (Submitted)
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic R Medicine (General)
spellingShingle R Medicine (General)
Chang Ching, Ching
Cloning and expression of ga protein of nip ah virus in yeast expression system
description Nipah virus (NiV) is a deadly zoonotic paramyxovirus that has emerged and reemerged over the last 10 years. In human, the infection leads to encephalitis with 92% mortality rate. This has underlined the importance for the development of a vaccine candidate in preventing such zoonotic disease. In previous study, the gene encoding the NiV glycoprotein (NiV -G) was cloned into the yeast Pichia Pastoris expression vector (pZMF) under the control of AOXI promoter. However, the recombinant G glycoprotein expression was found to be highly unstable in this system. Therefore, the stability of the recombinant protein was investigated by studying one of the truncated gene fragment (GA gene). The truncated gene encoding the gene sequence 1-867 was cloned and expressed in the same biological system. The detection of the truncated recombinant protein was performed through Western blot analysis. The result of this study indicated that the truncated fragment was stable and the expression was consistent with protein molecular weight of 40 kDa. The absent of degradation incident in this study had suggested that GA gene fragment was stable in full length G-glycoprotein expression. In addition, the truncated recombinant protein could be utilized as a promising vaccine candidate if the recombinant protein was found to be highly antigenic and immunogenic.
format Monograph
author Chang Ching, Ching
author_facet Chang Ching, Ching
author_sort Chang Ching, Ching
title Cloning and expression of ga protein of nip ah virus in yeast expression system
title_short Cloning and expression of ga protein of nip ah virus in yeast expression system
title_full Cloning and expression of ga protein of nip ah virus in yeast expression system
title_fullStr Cloning and expression of ga protein of nip ah virus in yeast expression system
title_full_unstemmed Cloning and expression of ga protein of nip ah virus in yeast expression system
title_sort cloning and expression of ga protein of nip ah virus in yeast expression system
publisher Universiti Sains Malaysia
publishDate 2008
url http://eprints.usm.my/48915/1/CHANG%20CHING%20CHING%20-%2024%20pages.pdf
http://eprints.usm.my/48915/
_version_ 1698697769541173248
score 13.211869

Similar Items