A study of human choline kinase a (hCK-a) in patients with ischemic heart disease (ihd) and heal thy volunteers
Ischemic heart disease (IHD) is predicted to become the dominant cause of mortality worldwide by 2030. As a silent yet eventually fatal disease, prevention and early detection through discovery of cardiac biomarker are the best resolution for IHD. Human choline kinase a (hCK-a) was investigated f...
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| Main Author: | |
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| Format: | Monograph |
| Language: | English |
| Published: |
Universiti Sains Malaysia
2008
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| Subjects: | |
| Online Access: | http://eprints.usm.my/48853/1/CHIA%20PHEE%20PHEE%20-%2024%20pages.pdf http://eprints.usm.my/48853/ |
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| Summary: | Ischemic heart disease (IHD) is predicted to become the dominant cause of
mortality worldwide by 2030. As a silent yet eventually fatal disease, prevention
and early detection through discovery of cardiac biomarker are the best resolution
for IHD. Human choline kinase a (hCK-a) was investigated for its possibility as a
cardiac biomarker in this study. The aim of this study is to investigate the
differences in the protein level and mRNA expression of hCK-a between IHD
patients and healthy controls. Blood samples from 10 IHD patients and 10 healthy
volunteers were analyzed for mRNA expression using RT-PCR. Western blot was
performed as a checkpoint for detection of hCK-a protein in blood. Subsequently,
ELISA test was conducted to measure the protein concentration of hCK-a protein.
C-reactive protein (CRP) was used as a standard reference in this study to provide
a parameter to distinguish between both study groups. Statistical analysis was
performed to investigate the relationship between hCK-a with IHD. Parametric test
was performed using independent t-test meanwhile non-parametric test was
performed using Mann-Whitney test. As anticipated, CRP was found to be
significant higher in expression both in mRNA level and protein level (Independent
t-test, p<0.001; Mann-Whitney test, p= 0.019). In this study, a significant lower
level of hCK-a2 mRNA expression in IHD patients was revealed (Independent ttest,
p=0.032). However, total hCK-a mRNA expression was found to have no
significant different between IHD patients and healthy volunteers (Mann-Whitney
test, p=0.705). Protein expression level of hCK-a was found to be significantly
lower in lHD patients as compared to healthy volunteers (Mann-Whitney test,
p=0.014). Albeit preliminary, this study found that there may be a profound
relationship between hCK-a and IHD. |
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