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Phenotypic And Proteomic Analysis Associated With Macrocyclic Lactones-Ivermectin Exposure In Caenorhabditis Elegans

Anthelmintic resistance has been reported in almost all species of parasites of livestock and involved all the major classes of broad-spectrum anthelmintic drug including ivermectin. Due to the study of ivermectin resistance in parasitic nematode is limited, thus, a free-living Caenorhabditis elegan...

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Main Author: Zain, Mariani Mohd
Format: Thesis
Language:English
Published: 2019
Subjects:
QH1 Natural history (General - Including nature conservation, geographical distribution)
Online Access:http://eprints.usm.my/48166/1/Mariani%20binti%20Mohd%20Zain%20cut.pdf
http://eprints.usm.my/48166/
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spelling my.usm.eprints.48166 http://eprints.usm.my/48166/ Phenotypic And Proteomic Analysis Associated With Macrocyclic Lactones-Ivermectin Exposure In Caenorhabditis Elegans Zain, Mariani Mohd QH1 Natural history (General - Including nature conservation, geographical distribution) Anthelmintic resistance has been reported in almost all species of parasites of livestock and involved all the major classes of broad-spectrum anthelmintic drug including ivermectin. Due to the study of ivermectin resistance in parasitic nematode is limited, thus, a free-living Caenorhabditis elegans was used as a model system in this study. The ivermectin sensitivity assay was carried out to investigate the response of wild-type C. elegans N2 strain to ivermectin exposure. Six in vitro assays were conducted; egg hatch assay, larval development assay, reproduce assay, thrashing assay, motility assay and pharyngeal pumping assay with IVM-resistant C. elegans DA1316 strain as a control. The results showed that ivermectin is effective on larvae but not on the eggs of wild-type C. elegans N2 strain. Ivermectin inhibits the larval development, egg-laying behavior, locomotion and feeding behavior of wild-type C. elegans N2 strain. Following the ivermectin sensitivity assay, the proteomic approach was carried out to identify the possible up-regulated and down-regulated protein expression of wild-type C. elegans N2 strain to ivermectin exposure. Two-dimensional gel electrophoresis was performed to compare the protein maps of the IVM-resistant C. elegans N2 strain (treated group) and wild-type C. elegans N2 strain (control group). Among the 25 selected protein spots, 18 protein spots were up-regulated and seven protein spots were down-regulated in the treated group compared to the control group. 2019-08 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/48166/1/Mariani%20binti%20Mohd%20Zain%20cut.pdf Zain, Mariani Mohd (2019) Phenotypic And Proteomic Analysis Associated With Macrocyclic Lactones-Ivermectin Exposure In Caenorhabditis Elegans. Masters thesis, Universiti Sains Malaysia.
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic QH1 Natural history (General - Including nature conservation, geographical distribution)
spellingShingle QH1 Natural history (General - Including nature conservation, geographical distribution)
Zain, Mariani Mohd
Phenotypic And Proteomic Analysis Associated With Macrocyclic Lactones-Ivermectin Exposure In Caenorhabditis Elegans
description Anthelmintic resistance has been reported in almost all species of parasites of livestock and involved all the major classes of broad-spectrum anthelmintic drug including ivermectin. Due to the study of ivermectin resistance in parasitic nematode is limited, thus, a free-living Caenorhabditis elegans was used as a model system in this study. The ivermectin sensitivity assay was carried out to investigate the response of wild-type C. elegans N2 strain to ivermectin exposure. Six in vitro assays were conducted; egg hatch assay, larval development assay, reproduce assay, thrashing assay, motility assay and pharyngeal pumping assay with IVM-resistant C. elegans DA1316 strain as a control. The results showed that ivermectin is effective on larvae but not on the eggs of wild-type C. elegans N2 strain. Ivermectin inhibits the larval development, egg-laying behavior, locomotion and feeding behavior of wild-type C. elegans N2 strain. Following the ivermectin sensitivity assay, the proteomic approach was carried out to identify the possible up-regulated and down-regulated protein expression of wild-type C. elegans N2 strain to ivermectin exposure. Two-dimensional gel electrophoresis was performed to compare the protein maps of the IVM-resistant C. elegans N2 strain (treated group) and wild-type C. elegans N2 strain (control group). Among the 25 selected protein spots, 18 protein spots were up-regulated and seven protein spots were down-regulated in the treated group compared to the control group.
format Thesis
author Zain, Mariani Mohd
author_facet Zain, Mariani Mohd
author_sort Zain, Mariani Mohd
title Phenotypic And Proteomic Analysis Associated With Macrocyclic Lactones-Ivermectin Exposure In Caenorhabditis Elegans
title_short Phenotypic And Proteomic Analysis Associated With Macrocyclic Lactones-Ivermectin Exposure In Caenorhabditis Elegans
title_full Phenotypic And Proteomic Analysis Associated With Macrocyclic Lactones-Ivermectin Exposure In Caenorhabditis Elegans
title_fullStr Phenotypic And Proteomic Analysis Associated With Macrocyclic Lactones-Ivermectin Exposure In Caenorhabditis Elegans
title_full_unstemmed Phenotypic And Proteomic Analysis Associated With Macrocyclic Lactones-Ivermectin Exposure In Caenorhabditis Elegans
title_sort phenotypic and proteomic analysis associated with macrocyclic lactones-ivermectin exposure in caenorhabditis elegans
publishDate 2019
url http://eprints.usm.my/48166/1/Mariani%20binti%20Mohd%20Zain%20cut.pdf
http://eprints.usm.my/48166/
_version_ 1690371958146859008
score 13.222552

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