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Detection 0f interleukin-10 polymorphism and plasmodium falciparum infecti0n ln ardamata idp camp al-geneina town Sudan

Malaria is the main cause of Sudanese morbidity and death. It contributes an estimated 7.5 million cases and 35,000 deaths annually, mainly because of Plasmodium falciparum (P. falciparum). Host and pathogen genetic factors are linked to the severity of the disease. In the promoter or coding region...

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Main Author: Osman, Abshir Ali
Format: Thesis
Language:English
Published: 2020
Subjects:
R Medicine
Online Access:http://eprints.usm.my/47964/1/21.%20Thesis_Final%20Copy_THESIS_ABSHIR%20ALI%20OSMAN_P-SKM0004_19-24%20pages.pdf
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spelling my.usm.eprints.47964 http://eprints.usm.my/47964/ Detection 0f interleukin-10 polymorphism and plasmodium falciparum infecti0n ln ardamata idp camp al-geneina town Sudan Osman, Abshir Ali R Medicine Malaria is the main cause of Sudanese morbidity and death. It contributes an estimated 7.5 million cases and 35,000 deaths annually, mainly because of Plasmodium falciparum (P. falciparum). Host and pathogen genetic factors are linked to the severity of the disease. In the promoter or coding region of cytokine genes, single nuclear polymorphisms (SNPs) alter their transcriptional activation and produce differential cytokine. Interleukin-10 (IL-10) is an anti-inflammatory cytokine that may play an active role in P. falciparum infection. IL-10 polymorphisms are associated with several diseases, including malaria. IL-10 has a highly polymorphic promoter with variations gene transcription, including 1082 G/A, 819 C/T, and 592 C/A. However, polymorphism at 1082 G/A gene is the most extensively studied because of this polymorphism's ability to increase IL-10 production, which correlated with susceptibility or protection against infection. Therefore, it is important to understand the association of P. falciparum infection and IL-10 gene polymorphism to confirm if this polymorphism plays a role in the incidence of P. falciparum infection to develop a more efficient vaccine against the disease. Polymerase Chain Response (PCR) is one of the most common methods for distinguishing malaria parasites from species levels and gene polymorphism detection in specific species. This study aimed at investigating the association of P. falciparum infection with IL-10 gene promoter 1082 G/A polymorphism in the Sudanese population. Thirty-four (34) blood smears of malarial patients from the Ardamata IDP Camp in Al-Geneina Town, Sudan, were enrolled in this study. The samples were first confirmed with nested PCR (nPCR) for Plasmodium infection before proceeding with P. falciparum identification. Further, PCR was then performed to evaluate IL-10 gene 1082 G/A polymorphism in the samples. nPCR showed that out of 34 samples, 17 were malaria positive, and 17 were negative. Interestingly, all the 17-malaria positive samples were confirmed as P. falciparum. Although nPCR has been claimed to be more sensitive and specific, the number of positive samples detected using this method was less than microscopy and ICT analysis. PCR analysis also confirmed that no gene mutation occurs in the malaria positive samples, indicating there was no significant association between IL-10 -1082G/A polymorphism and P. falciparum infection in these samples. 2020-08 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/47964/1/21.%20Thesis_Final%20Copy_THESIS_ABSHIR%20ALI%20OSMAN_P-SKM0004_19-24%20pages.pdf Osman, Abshir Ali (2020) Detection 0f interleukin-10 polymorphism and plasmodium falciparum infecti0n ln ardamata idp camp al-geneina town Sudan. Masters thesis, Universiti Sains Malaysia.
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic R Medicine
spellingShingle R Medicine
Osman, Abshir Ali
Detection 0f interleukin-10 polymorphism and plasmodium falciparum infecti0n ln ardamata idp camp al-geneina town Sudan
description Malaria is the main cause of Sudanese morbidity and death. It contributes an estimated 7.5 million cases and 35,000 deaths annually, mainly because of Plasmodium falciparum (P. falciparum). Host and pathogen genetic factors are linked to the severity of the disease. In the promoter or coding region of cytokine genes, single nuclear polymorphisms (SNPs) alter their transcriptional activation and produce differential cytokine. Interleukin-10 (IL-10) is an anti-inflammatory cytokine that may play an active role in P. falciparum infection. IL-10 polymorphisms are associated with several diseases, including malaria. IL-10 has a highly polymorphic promoter with variations gene transcription, including 1082 G/A, 819 C/T, and 592 C/A. However, polymorphism at 1082 G/A gene is the most extensively studied because of this polymorphism's ability to increase IL-10 production, which correlated with susceptibility or protection against infection. Therefore, it is important to understand the association of P. falciparum infection and IL-10 gene polymorphism to confirm if this polymorphism plays a role in the incidence of P. falciparum infection to develop a more efficient vaccine against the disease. Polymerase Chain Response (PCR) is one of the most common methods for distinguishing malaria parasites from species levels and gene polymorphism detection in specific species. This study aimed at investigating the association of P. falciparum infection with IL-10 gene promoter 1082 G/A polymorphism in the Sudanese population. Thirty-four (34) blood smears of malarial patients from the Ardamata IDP Camp in Al-Geneina Town, Sudan, were enrolled in this study. The samples were first confirmed with nested PCR (nPCR) for Plasmodium infection before proceeding with P. falciparum identification. Further, PCR was then performed to evaluate IL-10 gene 1082 G/A polymorphism in the samples. nPCR showed that out of 34 samples, 17 were malaria positive, and 17 were negative. Interestingly, all the 17-malaria positive samples were confirmed as P. falciparum. Although nPCR has been claimed to be more sensitive and specific, the number of positive samples detected using this method was less than microscopy and ICT analysis. PCR analysis also confirmed that no gene mutation occurs in the malaria positive samples, indicating there was no significant association between IL-10 -1082G/A polymorphism and P. falciparum infection in these samples.
format Thesis
author Osman, Abshir Ali
author_facet Osman, Abshir Ali
author_sort Osman, Abshir Ali
title Detection 0f interleukin-10 polymorphism and plasmodium falciparum infecti0n ln ardamata idp camp al-geneina town Sudan
title_short Detection 0f interleukin-10 polymorphism and plasmodium falciparum infecti0n ln ardamata idp camp al-geneina town Sudan
title_full Detection 0f interleukin-10 polymorphism and plasmodium falciparum infecti0n ln ardamata idp camp al-geneina town Sudan
title_fullStr Detection 0f interleukin-10 polymorphism and plasmodium falciparum infecti0n ln ardamata idp camp al-geneina town Sudan
title_full_unstemmed Detection 0f interleukin-10 polymorphism and plasmodium falciparum infecti0n ln ardamata idp camp al-geneina town Sudan
title_sort detection 0f interleukin-10 polymorphism and plasmodium falciparum infecti0n ln ardamata idp camp al-geneina town sudan
publishDate 2020
url http://eprints.usm.my/47964/1/21.%20Thesis_Final%20Copy_THESIS_ABSHIR%20ALI%20OSMAN_P-SKM0004_19-24%20pages.pdf
http://eprints.usm.my/47964/
_version_ 1685579682720776192
score 13.223943

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