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Mutation analysis of apyrase gene of shigella jlexneri - towards the development of vaccine strain

The discovery of the microbe Shigella was dated back about I 00 years ago by a scientist named Kiyoshi Shiga. Virulent Shigella spp. are known to cause bacillary dysentery (shigeilosis) in humans which is characterized by the passage of bloody stools. According to the WHO, an estimated of 1.1 mil...

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Main Author: Aun, Seah Chin
Format: Monograph
Language:English
Published: Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia 2005
Subjects:
R Medicine
Online Access:http://eprints.usm.my/47796/1/SEAH%20CHIN%20AUN-24%20pages.pdf
http://eprints.usm.my/47796/
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spelling my.usm.eprints.47796 http://eprints.usm.my/47796/ Mutation analysis of apyrase gene of shigella jlexneri - towards the development of vaccine strain Aun, Seah Chin R Medicine The discovery of the microbe Shigella was dated back about I 00 years ago by a scientist named Kiyoshi Shiga. Virulent Shigella spp. are known to cause bacillary dysentery (shigeilosis) in humans which is characterized by the passage of bloody stools. According to the WHO, an estimated of 1.1 million global population dies every year is due to the disease shigellosis. Despite much studies were carried out regarding this pathogenic bacteria, no vaccine is available for this infectious disease. Literatures have postulated that one of the virulence genes is the apy gene which encodes for apyrase. Therefore, in this study the gene of interest is the apy gene. We have successfully mutated the apy gene with a kanamycin gene cassette (aphA) and subcloned the construct into a conjugative suicide vector. Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia 2005-03 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/47796/1/SEAH%20CHIN%20AUN-24%20pages.pdf Aun, Seah Chin (2005) Mutation analysis of apyrase gene of shigella jlexneri - towards the development of vaccine strain. Other. Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia. (Submitted)
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic R Medicine
spellingShingle R Medicine
Aun, Seah Chin
Mutation analysis of apyrase gene of shigella jlexneri - towards the development of vaccine strain
description The discovery of the microbe Shigella was dated back about I 00 years ago by a scientist named Kiyoshi Shiga. Virulent Shigella spp. are known to cause bacillary dysentery (shigeilosis) in humans which is characterized by the passage of bloody stools. According to the WHO, an estimated of 1.1 million global population dies every year is due to the disease shigellosis. Despite much studies were carried out regarding this pathogenic bacteria, no vaccine is available for this infectious disease. Literatures have postulated that one of the virulence genes is the apy gene which encodes for apyrase. Therefore, in this study the gene of interest is the apy gene. We have successfully mutated the apy gene with a kanamycin gene cassette (aphA) and subcloned the construct into a conjugative suicide vector.
format Monograph
author Aun, Seah Chin
author_facet Aun, Seah Chin
author_sort Aun, Seah Chin
title Mutation analysis of apyrase gene of shigella jlexneri - towards the development of vaccine strain
title_short Mutation analysis of apyrase gene of shigella jlexneri - towards the development of vaccine strain
title_full Mutation analysis of apyrase gene of shigella jlexneri - towards the development of vaccine strain
title_fullStr Mutation analysis of apyrase gene of shigella jlexneri - towards the development of vaccine strain
title_full_unstemmed Mutation analysis of apyrase gene of shigella jlexneri - towards the development of vaccine strain
title_sort mutation analysis of apyrase gene of shigella jlexneri - towards the development of vaccine strain
publisher Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia
publishDate 2005
url http://eprints.usm.my/47796/1/SEAH%20CHIN%20AUN-24%20pages.pdf
http://eprints.usm.my/47796/
_version_ 1683231607085858816
score 13.211869

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