PCR-RDB assay for detection of vibrio cholerae

A colorimetric detection method has been designed and optimized by immobilizing a specific DNA probe onto Biodyne C membrane and hybridize it with heat denatured biotin labeled PCR amplicon to detect the presence of the hemM gene of Vibrio cholerae. Therefore it can be used to replaced agarose g...

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Bibliographic Details
Main Author: Yun, Foong Sui
Format: Thesis
Language:English
Published: Universiti Sains Malaysia 2005
Subjects:
Online Access:http://eprints.usm.my/47694/1/PTA...Foong%20Sui%20Yun...2005...mka.-24%20pages.pdf
http://eprints.usm.my/47694/
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