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Mutation analysis of apyrase gene of Shigella Flexneri- towards the development of vaccine strain

The discovery of the microbe Shigella was dated back about 100 years ago by a scientist named Kiyoshi Shiga. Virulent Shigella spp. are known to cause bacillary dysentery (shigellosis) in humans which is characterized by the passage of bloody stools. According to the WHO, an estimated of 1.1 mill...

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Main Author: Aun, Seah Chin
Format: Thesis
Language:English
Published: 2005
Subjects:
R Medicine
Online Access:http://eprints.usm.my/47503/1/Seah%20Chin%20Aun-24%20pages.pdf
http://eprints.usm.my/47503/
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spelling my.usm.eprints.47503 http://eprints.usm.my/47503/ Mutation analysis of apyrase gene of Shigella Flexneri- towards the development of vaccine strain Aun, Seah Chin R Medicine The discovery of the microbe Shigella was dated back about 100 years ago by a scientist named Kiyoshi Shiga. Virulent Shigella spp. are known to cause bacillary dysentery (shigellosis) in humans which is characterized by the passage of bloody stools. According to the WHO, an estimated of 1.1 million global population dies every year is due to the disease shigellosis. Despite much studies were carried out regarding this pathogenic bacteria, no vaccine is available for this infectious disease. Literatures have postulated that one of the virulence genes is the apy gene which encodes for apyrase. Therefore, in this study the gene of interest is the apy gene. We have successfully mutated the apy gene with a kanamycin gene cassette (aphA) and subcloned the construct into a conjugative suicide vector. The use of this antibiotic gene cassette is to facilitate the process of manipulating the genes as it serves as a selective marker. The creation of the construct (apy::aphA) is to facilitated the approach into the development of a potential vaccine strain of Shigella; as literatures have indicated that apy gene is one of the virulence genes. Furthermore, mutation of the virulence genes has been proven to be highly immunogenic and safe. 2005-03 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/47503/1/Seah%20Chin%20Aun-24%20pages.pdf Aun, Seah Chin (2005) Mutation analysis of apyrase gene of Shigella Flexneri- towards the development of vaccine strain. Masters thesis, Universiti Sains Malaysia.
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic R Medicine
spellingShingle R Medicine
Aun, Seah Chin
Mutation analysis of apyrase gene of Shigella Flexneri- towards the development of vaccine strain
description The discovery of the microbe Shigella was dated back about 100 years ago by a scientist named Kiyoshi Shiga. Virulent Shigella spp. are known to cause bacillary dysentery (shigellosis) in humans which is characterized by the passage of bloody stools. According to the WHO, an estimated of 1.1 million global population dies every year is due to the disease shigellosis. Despite much studies were carried out regarding this pathogenic bacteria, no vaccine is available for this infectious disease. Literatures have postulated that one of the virulence genes is the apy gene which encodes for apyrase. Therefore, in this study the gene of interest is the apy gene. We have successfully mutated the apy gene with a kanamycin gene cassette (aphA) and subcloned the construct into a conjugative suicide vector. The use of this antibiotic gene cassette is to facilitate the process of manipulating the genes as it serves as a selective marker. The creation of the construct (apy::aphA) is to facilitated the approach into the development of a potential vaccine strain of Shigella; as literatures have indicated that apy gene is one of the virulence genes. Furthermore, mutation of the virulence genes has been proven to be highly immunogenic and safe.
format Thesis
author Aun, Seah Chin
author_facet Aun, Seah Chin
author_sort Aun, Seah Chin
title Mutation analysis of apyrase gene of Shigella Flexneri- towards the development of vaccine strain
title_short Mutation analysis of apyrase gene of Shigella Flexneri- towards the development of vaccine strain
title_full Mutation analysis of apyrase gene of Shigella Flexneri- towards the development of vaccine strain
title_fullStr Mutation analysis of apyrase gene of Shigella Flexneri- towards the development of vaccine strain
title_full_unstemmed Mutation analysis of apyrase gene of Shigella Flexneri- towards the development of vaccine strain
title_sort mutation analysis of apyrase gene of shigella flexneri- towards the development of vaccine strain
publishDate 2005
url http://eprints.usm.my/47503/1/Seah%20Chin%20Aun-24%20pages.pdf
http://eprints.usm.my/47503/
_version_ 1681490226262835200
score 13.211869

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