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Survey Of Natural Distribution Of Black Yeasts In Malaysia With Special Emphasize On Exophiala Dermatitidis

Black yeasts are ecologically remarkable as they can live in extreme, unusual and toxic environments. Exophiala dermatitidis, a human opportunistic pathogen was previously reported to be isolated from sauna facilities, oak sleepers of railway ties, faeces of frugivorous birds and bats, land contamin...

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Main Author: Jayaram, Mehalene
Format: Thesis
Language:English
Published: 2019
Subjects:
H1-99 Social sciences (General)
Online Access:http://eprints.usm.my/46618/1/Mehalene%20Msc%20PDF24.pdf
http://eprints.usm.my/46618/
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spelling my.usm.eprints.46618 http://eprints.usm.my/46618/ Survey Of Natural Distribution Of Black Yeasts In Malaysia With Special Emphasize On Exophiala Dermatitidis Jayaram, Mehalene H1-99 Social sciences (General) Black yeasts are ecologically remarkable as they can live in extreme, unusual and toxic environments. Exophiala dermatitidis, a human opportunistic pathogen was previously reported to be isolated from sauna facilities, oak sleepers of railway ties, faeces of frugivorous birds and bats, land contaminated with waste petrol hydrocarbons and dishwashers worldwide. Cryptococcus neoformans, on the other hand, was isolated from tree trunk hollows, Eucalyptus trees and faeces of birds. Both of these yeasts were frequently isolated from tropical regions of the world. As there are limited reports of environmental isolation of these black yeasts in Malaysia, their natural distribution in the environment were surveyed in this study. Forty-seven strains of Exophiala spp. and four strains of Cryptococcus spp. were isolated from various locations in Malaysia. As Exophiala spp. are dimorphic fungi, the colonies were grown under different conditions to identify the yeast and hyphal growth of the black fungi. Urea hydrolysis, DNAse test and proteinase activity were also conducted to test their physiological properties. The black yeasts were characterised by the type of conidiogenous cells, and chlamydospores produced and their identity confirmed by sequencing of the ITS region. Overall, 22 strains of E. dermatitidis were successfully identified from railway track stones (n = 5), burnt tree bark (n = 2), oil dripped soil sample (n = 5), hot spring biofilm (n = 1), tar road contaminated with petrol hydrocarbon (n = 4), pigeon droppings (n = 2), drain and deep mud of Sungai Pinang (n = 3). 2019-04 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/46618/1/Mehalene%20Msc%20PDF24.pdf Jayaram, Mehalene (2019) Survey Of Natural Distribution Of Black Yeasts In Malaysia With Special Emphasize On Exophiala Dermatitidis. Masters thesis, Universiti Sains Malaysia.
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic H1-99 Social sciences (General)
spellingShingle H1-99 Social sciences (General)
Jayaram, Mehalene
Survey Of Natural Distribution Of Black Yeasts In Malaysia With Special Emphasize On Exophiala Dermatitidis
description Black yeasts are ecologically remarkable as they can live in extreme, unusual and toxic environments. Exophiala dermatitidis, a human opportunistic pathogen was previously reported to be isolated from sauna facilities, oak sleepers of railway ties, faeces of frugivorous birds and bats, land contaminated with waste petrol hydrocarbons and dishwashers worldwide. Cryptococcus neoformans, on the other hand, was isolated from tree trunk hollows, Eucalyptus trees and faeces of birds. Both of these yeasts were frequently isolated from tropical regions of the world. As there are limited reports of environmental isolation of these black yeasts in Malaysia, their natural distribution in the environment were surveyed in this study. Forty-seven strains of Exophiala spp. and four strains of Cryptococcus spp. were isolated from various locations in Malaysia. As Exophiala spp. are dimorphic fungi, the colonies were grown under different conditions to identify the yeast and hyphal growth of the black fungi. Urea hydrolysis, DNAse test and proteinase activity were also conducted to test their physiological properties. The black yeasts were characterised by the type of conidiogenous cells, and chlamydospores produced and their identity confirmed by sequencing of the ITS region. Overall, 22 strains of E. dermatitidis were successfully identified from railway track stones (n = 5), burnt tree bark (n = 2), oil dripped soil sample (n = 5), hot spring biofilm (n = 1), tar road contaminated with petrol hydrocarbon (n = 4), pigeon droppings (n = 2), drain and deep mud of Sungai Pinang (n = 3).
format Thesis
author Jayaram, Mehalene
author_facet Jayaram, Mehalene
author_sort Jayaram, Mehalene
title Survey Of Natural Distribution Of Black Yeasts In Malaysia With Special Emphasize On Exophiala Dermatitidis
title_short Survey Of Natural Distribution Of Black Yeasts In Malaysia With Special Emphasize On Exophiala Dermatitidis
title_full Survey Of Natural Distribution Of Black Yeasts In Malaysia With Special Emphasize On Exophiala Dermatitidis
title_fullStr Survey Of Natural Distribution Of Black Yeasts In Malaysia With Special Emphasize On Exophiala Dermatitidis
title_full_unstemmed Survey Of Natural Distribution Of Black Yeasts In Malaysia With Special Emphasize On Exophiala Dermatitidis
title_sort survey of natural distribution of black yeasts in malaysia with special emphasize on exophiala dermatitidis
publishDate 2019
url http://eprints.usm.my/46618/1/Mehalene%20Msc%20PDF24.pdf
http://eprints.usm.my/46618/
_version_ 1671340449771552768
score 13.211869

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