Investigation Of Irreversible Bonding Between Polydimethylsiloxane And Printed Circuit Board For Designing Leakage-Free Dna Biochip
One of the issues in designing a disposable DNA biochip based on capillary electrophoresis technology is the leakage of fluid in the microchannel though small gaps between electrodes. In this work a leakagefree and reusable biochip is designed for DNA separation and detection applications. The b...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English |
| Published: |
2017
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| Subjects: | |
| Online Access: | http://eprints.usm.my/46440/1/Investigation%20Of%20Irreversible%20Bonding%20Between%20Polydimethylsiloxane%20And%20Printed%20Circuit%20Board%20For%20Designing%20Leakage-Free%20Dna%20Biochip.pdf http://eprints.usm.my/46440/ |
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| Summary: | One of the issues in designing a disposable DNA biochip based on
capillary electrophoresis technology is the leakage of fluid in the
microchannel though small gaps between electrodes. In this work a leakagefree
and reusable biochip is designed for DNA separation and detection
applications. The biochip comprises PDMS microfluidic structure fabricated
with soft-lithography and copper electrodes which are engraved on FR-4
board with standard semi-automatic processes. An inhibitive layer made
from photocurable diacrylate bisphenol A polymer (DABA) is used to
establish irreversible bonding between PDMS and PCB substrates. Pull-off
tests resulted in an average tensile strength of 287.357 kPa and standard
deviation ± 23.793 kPa. These results are comparable to PDMS–PDMS
bonding via conventional oxygen plasma and corona discharge. Meanwhile
the leakage test showed that the microchannel could withstand pressure of
more than 189 kPa which is sufficiently high for most biochip applications.
Finally experiments performed on single DNA band produced by using PCR
and multiple bands from standard DNA ladders indicated that the proposed
design can accurately separate DNA fragments with current sensitivity
consistently higher than 100 nA and at electric field strength of 20V/cm.
Comparing to the previous design that used clips to mechanically clamp
PDMS and PCB substrates, the new approach effectively seals the device,
thus preventing leakage of liquid from the sensor matrix. This together with
the electrochemically inert characteristics of the photopolymer inhibitor,
open up possibilities in designing a truly portable bio-sensing device. |
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