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Therapeutic Potential Of Menstrual Blood-Derived Endometrium Stem Cells On In Vitro And In Vivo Parkinson's Disease Models

Human menstrual blood-derived endometrial stem cells (MenSCs) have shown therapeutic potential on various diseases by immunoregulation and tissue regeneration. However, their effects on Parkinson’s disease (PD) remain unknown. The aim of this study was to evaluate the protective function of MenSCs a...

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Main Author: Li, Han
Format: Thesis
Language:English
Published: 2019
Subjects:
R Medicine (General)
Online Access:http://eprints.usm.my/46399/1/Li%20Han_HJ.pdf
http://eprints.usm.my/46399/
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spelling my.usm.eprints.46399 http://eprints.usm.my/46399/ Therapeutic Potential Of Menstrual Blood-Derived Endometrium Stem Cells On In Vitro And In Vivo Parkinson's Disease Models Li, Han R Medicine (General) Human menstrual blood-derived endometrial stem cells (MenSCs) have shown therapeutic potential on various diseases by immunoregulation and tissue regeneration. However, their effects on Parkinson’s disease (PD) remain unknown. The aim of this study was to evaluate the protective function of MenSCs and their derivatives on in vitro and in vivo PD models. Neuroblastoma cell line (SH-SY5Y) and mouse midbrain slice were exposed to 1-methyl-4-phenylpyridinium (MPP+) to establish in vitro level PD models. Then, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) was used to construct PD mouse model by intraperitoneal injection. After co-culture in vitro PD models with conditioned medium of MenSCs (MenSCs-CM), the viability of cell and midbrain slice were detected by Prestoblue and lactate dehydrogenase assay. The expression of inflammatory genes, anti-oxidant and apoptosis-related genes were detected by qRT-PCR. Dihydroethidium, Rhodamine123, and Annexin V/PI staining were used to detect reactive oxygen species (ROS), mitochondrial membrane potential, and cell apoptosis, respectively. Protein array was conducted to analyze factors inside MenSCs-CM. Moreover, MenSCs were transplanted to striatum (Str) region of PD mouse brain using a stereotaxic instrument. Survival time of MenSCs, dopamine (DA) level, expression of inflammatory genes and anti-oxidant genes were evaluated. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis was used to analyze pathways. 2019-08 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/46399/1/Li%20Han_HJ.pdf Li, Han (2019) Therapeutic Potential Of Menstrual Blood-Derived Endometrium Stem Cells On In Vitro And In Vivo Parkinson's Disease Models. PhD thesis, Universiti Sains Malaysia.
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic R Medicine (General)
spellingShingle R Medicine (General)
Li, Han
Therapeutic Potential Of Menstrual Blood-Derived Endometrium Stem Cells On In Vitro And In Vivo Parkinson's Disease Models
description Human menstrual blood-derived endometrial stem cells (MenSCs) have shown therapeutic potential on various diseases by immunoregulation and tissue regeneration. However, their effects on Parkinson’s disease (PD) remain unknown. The aim of this study was to evaluate the protective function of MenSCs and their derivatives on in vitro and in vivo PD models. Neuroblastoma cell line (SH-SY5Y) and mouse midbrain slice were exposed to 1-methyl-4-phenylpyridinium (MPP+) to establish in vitro level PD models. Then, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) was used to construct PD mouse model by intraperitoneal injection. After co-culture in vitro PD models with conditioned medium of MenSCs (MenSCs-CM), the viability of cell and midbrain slice were detected by Prestoblue and lactate dehydrogenase assay. The expression of inflammatory genes, anti-oxidant and apoptosis-related genes were detected by qRT-PCR. Dihydroethidium, Rhodamine123, and Annexin V/PI staining were used to detect reactive oxygen species (ROS), mitochondrial membrane potential, and cell apoptosis, respectively. Protein array was conducted to analyze factors inside MenSCs-CM. Moreover, MenSCs were transplanted to striatum (Str) region of PD mouse brain using a stereotaxic instrument. Survival time of MenSCs, dopamine (DA) level, expression of inflammatory genes and anti-oxidant genes were evaluated. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis was used to analyze pathways.
format Thesis
author Li, Han
author_facet Li, Han
author_sort Li, Han
title Therapeutic Potential Of Menstrual Blood-Derived Endometrium Stem Cells On In Vitro And In Vivo Parkinson's Disease Models
title_short Therapeutic Potential Of Menstrual Blood-Derived Endometrium Stem Cells On In Vitro And In Vivo Parkinson's Disease Models
title_full Therapeutic Potential Of Menstrual Blood-Derived Endometrium Stem Cells On In Vitro And In Vivo Parkinson's Disease Models
title_fullStr Therapeutic Potential Of Menstrual Blood-Derived Endometrium Stem Cells On In Vitro And In Vivo Parkinson's Disease Models
title_full_unstemmed Therapeutic Potential Of Menstrual Blood-Derived Endometrium Stem Cells On In Vitro And In Vivo Parkinson's Disease Models
title_sort therapeutic potential of menstrual blood-derived endometrium stem cells on in vitro and in vivo parkinson's disease models
publishDate 2019
url http://eprints.usm.my/46399/1/Li%20Han_HJ.pdf
http://eprints.usm.my/46399/
_version_ 1662755779337256960
score 13.211869

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