Cover Image

Quantification Of Endogenous Anabolic Androgenic Steroids Using Gas Chromatography Tandem Mass Spectrometry: An Application Of Steroid Profile From Eurycoma Longifolia Administration

A method was developed using gas chromatography tandem mass spectrometry (GC/MS/MS) to quantify the levels of endogenous androgenic anabolic steroids (EAAS) including testosterone, dehydroepiandrosterone, 4-andro -stenedione, 5-androstenediol, dihydrotestosterone and other related metabolites like a...

Full description

Saved in:
Bibliographic Details
Main Author: Abushareeda, Wadha Masoud
Format: Thesis
Language:English
Published: 2011
Subjects:
R5-920 Medicine (General)
Online Access:http://eprints.usm.my/46250/1/Wadha%20Masoud%20Abushareeda_HJ.pdf
http://eprints.usm.my/46250/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.usm.eprints.46250
record_format eprints
spelling my.usm.eprints.46250 http://eprints.usm.my/46250/ Quantification Of Endogenous Anabolic Androgenic Steroids Using Gas Chromatography Tandem Mass Spectrometry: An Application Of Steroid Profile From Eurycoma Longifolia Administration Abushareeda, Wadha Masoud R5-920 Medicine (General) A method was developed using gas chromatography tandem mass spectrometry (GC/MS/MS) to quantify the levels of endogenous androgenic anabolic steroids (EAAS) including testosterone, dehydroepiandrosterone, 4-andro -stenedione, 5-androstenediol, dihydrotestosterone and other related metabolites like androsterone, etiocholanolone, 5-α-androstandiol and 5-β-androstandiol in urine samples. The steroids were extracted using SPE method followed by LLE and analyzed by GC/MS/MS. Multiple Reaction Monitoring (MRM) was adopted for the analysis, using two MRM transitions as the qualifier ions and one MRM transition as the quantifier ion for each analyte. The linearity, extraction recovery, LOD, intra- and inter–day precision and accuracy as well as robustness of the method were validated. The correlation coefficients (r2) obtained was higher than or equal to 0.993 for all the compounds. The recoveries achieved using the method were higher than 70% for all analytes The limits of detection that was obtained using the method described in the present study ranged from 0.1 to 0.2 ng/mL. The limits of quantification ranged from to 0.5 to 1 ng/mL. The results showed good precision; where CV < 8% and accuracy ≥ 80% for all compounds in all assayed levels. The validation results showed that the analytical method used in this study to quantify the investigated anabolic steroids in urine has complied with the criteria of the validation for quantitative methods according to WADA ISL and NATA Technical Note. 2011 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/46250/1/Wadha%20Masoud%20Abushareeda_HJ.pdf Abushareeda, Wadha Masoud (2011) Quantification Of Endogenous Anabolic Androgenic Steroids Using Gas Chromatography Tandem Mass Spectrometry: An Application Of Steroid Profile From Eurycoma Longifolia Administration. Masters thesis, Universiti Sains Malaysia.
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic R5-920 Medicine (General)
spellingShingle R5-920 Medicine (General)
Abushareeda, Wadha Masoud
Quantification Of Endogenous Anabolic Androgenic Steroids Using Gas Chromatography Tandem Mass Spectrometry: An Application Of Steroid Profile From Eurycoma Longifolia Administration
description A method was developed using gas chromatography tandem mass spectrometry (GC/MS/MS) to quantify the levels of endogenous androgenic anabolic steroids (EAAS) including testosterone, dehydroepiandrosterone, 4-andro -stenedione, 5-androstenediol, dihydrotestosterone and other related metabolites like androsterone, etiocholanolone, 5-α-androstandiol and 5-β-androstandiol in urine samples. The steroids were extracted using SPE method followed by LLE and analyzed by GC/MS/MS. Multiple Reaction Monitoring (MRM) was adopted for the analysis, using two MRM transitions as the qualifier ions and one MRM transition as the quantifier ion for each analyte. The linearity, extraction recovery, LOD, intra- and inter–day precision and accuracy as well as robustness of the method were validated. The correlation coefficients (r2) obtained was higher than or equal to 0.993 for all the compounds. The recoveries achieved using the method were higher than 70% for all analytes The limits of detection that was obtained using the method described in the present study ranged from 0.1 to 0.2 ng/mL. The limits of quantification ranged from to 0.5 to 1 ng/mL. The results showed good precision; where CV < 8% and accuracy ≥ 80% for all compounds in all assayed levels. The validation results showed that the analytical method used in this study to quantify the investigated anabolic steroids in urine has complied with the criteria of the validation for quantitative methods according to WADA ISL and NATA Technical Note.
format Thesis
author Abushareeda, Wadha Masoud
author_facet Abushareeda, Wadha Masoud
author_sort Abushareeda, Wadha Masoud
title Quantification Of Endogenous Anabolic Androgenic Steroids Using Gas Chromatography Tandem Mass Spectrometry: An Application Of Steroid Profile From Eurycoma Longifolia Administration
title_short Quantification Of Endogenous Anabolic Androgenic Steroids Using Gas Chromatography Tandem Mass Spectrometry: An Application Of Steroid Profile From Eurycoma Longifolia Administration
title_full Quantification Of Endogenous Anabolic Androgenic Steroids Using Gas Chromatography Tandem Mass Spectrometry: An Application Of Steroid Profile From Eurycoma Longifolia Administration
title_fullStr Quantification Of Endogenous Anabolic Androgenic Steroids Using Gas Chromatography Tandem Mass Spectrometry: An Application Of Steroid Profile From Eurycoma Longifolia Administration
title_full_unstemmed Quantification Of Endogenous Anabolic Androgenic Steroids Using Gas Chromatography Tandem Mass Spectrometry: An Application Of Steroid Profile From Eurycoma Longifolia Administration
title_sort quantification of endogenous anabolic androgenic steroids using gas chromatography tandem mass spectrometry: an application of steroid profile from eurycoma longifolia administration
publishDate 2011
url http://eprints.usm.my/46250/1/Wadha%20Masoud%20Abushareeda_HJ.pdf
http://eprints.usm.my/46250/
_version_ 1662755759113371648
score 13.211869

Similar Items