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Chicken Feathers As An Alternative Substrate For Extracellular Keratinase Production Using Microsporum Fulvum Ibrl Sd3 By Solid Substrate Fermentation

In nature, chicken feathers were abundantly generated from poultry industry and become severe environmental problems. The capabilities of microbial degradation and bioconversion of chicken feathers were directed to the reason for this study. The present study used the keratinaceous waste as a substr...

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Main Author: Alyas, Nur Diyana
Format: Thesis
Language:English
Published: 2013
Subjects:
QH1 Natural history (General - Including nature conservation, geographical distribution)
Online Access:http://eprints.usm.my/45260/1/Nur%20Diyana%20Binti%20Alyas24.pdf
http://eprints.usm.my/45260/
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spelling my.usm.eprints.45260 http://eprints.usm.my/45260/ Chicken Feathers As An Alternative Substrate For Extracellular Keratinase Production Using Microsporum Fulvum Ibrl Sd3 By Solid Substrate Fermentation Alyas, Nur Diyana QH1 Natural history (General - Including nature conservation, geographical distribution) In nature, chicken feathers were abundantly generated from poultry industry and become severe environmental problems. The capabilities of microbial degradation and bioconversion of chicken feathers were directed to the reason for this study. The present study used the keratinaceous waste as a substrate in keratinase production from molecular identification of M. fulvum IBRL SD3 via solid substrate fermentation. The improvements of cultural conditions for maximal keratinase production in a shake flask system with particles size of substrate of 0.75 mm, at the initial moisture content of 100% (w/w), cultivation temperature of room temperature (30±2ºC), initial pH 7, mixing frequency at once every 24 hours and inoculum size of 1 X 107 spores/ml showed keratinase achieved 0.266 U/g of fermented substrate on day 6th of cultivation. In the improvement of medium compositions, no additional carbon source was required and a slight supplementation of 0.70% (w/w) of yeast extract to produce the maximum keratinase activity on day 6th of cultivation at 0.372 U/g of fermented substrate with the increment of keratinase activity at 905.41% compared to the initial profile. Furthermore, an improvement of tray system was conducted using a shallow aluminium tray (16 cm x 16 cm x 5 cm). 2013-07 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/45260/1/Nur%20Diyana%20Binti%20Alyas24.pdf Alyas, Nur Diyana (2013) Chicken Feathers As An Alternative Substrate For Extracellular Keratinase Production Using Microsporum Fulvum Ibrl Sd3 By Solid Substrate Fermentation. Masters thesis, Universiti Sains Malaysia.
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic QH1 Natural history (General - Including nature conservation, geographical distribution)
spellingShingle QH1 Natural history (General - Including nature conservation, geographical distribution)
Alyas, Nur Diyana
Chicken Feathers As An Alternative Substrate For Extracellular Keratinase Production Using Microsporum Fulvum Ibrl Sd3 By Solid Substrate Fermentation
description In nature, chicken feathers were abundantly generated from poultry industry and become severe environmental problems. The capabilities of microbial degradation and bioconversion of chicken feathers were directed to the reason for this study. The present study used the keratinaceous waste as a substrate in keratinase production from molecular identification of M. fulvum IBRL SD3 via solid substrate fermentation. The improvements of cultural conditions for maximal keratinase production in a shake flask system with particles size of substrate of 0.75 mm, at the initial moisture content of 100% (w/w), cultivation temperature of room temperature (30±2ºC), initial pH 7, mixing frequency at once every 24 hours and inoculum size of 1 X 107 spores/ml showed keratinase achieved 0.266 U/g of fermented substrate on day 6th of cultivation. In the improvement of medium compositions, no additional carbon source was required and a slight supplementation of 0.70% (w/w) of yeast extract to produce the maximum keratinase activity on day 6th of cultivation at 0.372 U/g of fermented substrate with the increment of keratinase activity at 905.41% compared to the initial profile. Furthermore, an improvement of tray system was conducted using a shallow aluminium tray (16 cm x 16 cm x 5 cm).
format Thesis
author Alyas, Nur Diyana
author_facet Alyas, Nur Diyana
author_sort Alyas, Nur Diyana
title Chicken Feathers As An Alternative Substrate For Extracellular Keratinase Production Using Microsporum Fulvum Ibrl Sd3 By Solid Substrate Fermentation
title_short Chicken Feathers As An Alternative Substrate For Extracellular Keratinase Production Using Microsporum Fulvum Ibrl Sd3 By Solid Substrate Fermentation
title_full Chicken Feathers As An Alternative Substrate For Extracellular Keratinase Production Using Microsporum Fulvum Ibrl Sd3 By Solid Substrate Fermentation
title_fullStr Chicken Feathers As An Alternative Substrate For Extracellular Keratinase Production Using Microsporum Fulvum Ibrl Sd3 By Solid Substrate Fermentation
title_full_unstemmed Chicken Feathers As An Alternative Substrate For Extracellular Keratinase Production Using Microsporum Fulvum Ibrl Sd3 By Solid Substrate Fermentation
title_sort chicken feathers as an alternative substrate for extracellular keratinase production using microsporum fulvum ibrl sd3 by solid substrate fermentation
publishDate 2013
url http://eprints.usm.my/45260/1/Nur%20Diyana%20Binti%20Alyas24.pdf
http://eprints.usm.my/45260/
_version_ 1643711235169976320
score 13.211869

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