Cover Image

Antifungal effect of Malaysian aloe vera leaf extract selected fungal species of pathologenic otomycosis in in-vitro culture medium

Introduction: Aloe barbadensis miller or Aloe vera has been used for therapeutic purposes since ancient time. Antifungal activity is amongst the medicinal properties owned by Aloe vera. This study proposes antifungal effect of Malaysian Aloe vera leaf extract on pathogenic otomycosis species, Asp...

Full description

Saved in:
Bibliographic Details
Main Author: Saniasiaya, Jeyasakthy
Format: Thesis
Language:English
Published: 2017
Subjects:
RB Pathology
Online Access:http://eprints.usm.my/44982/1/Dr.%20Jeyasakthy%20Saniasiya-24%20pages.pdf
http://eprints.usm.my/44982/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.usm.eprints.44982
record_format eprints
spelling my.usm.eprints.44982 http://eprints.usm.my/44982/ Antifungal effect of Malaysian aloe vera leaf extract selected fungal species of pathologenic otomycosis in in-vitro culture medium Saniasiaya, Jeyasakthy RB Pathology Introduction: Aloe barbadensis miller or Aloe vera has been used for therapeutic purposes since ancient time. Antifungal activity is amongst the medicinal properties owned by Aloe vera. This study proposes antifungal effect of Malaysian Aloe vera leaf extract on pathogenic otomycosis species, Aspergillus niger and Candida albicans. Methodology: This pilot study is a laboratory-controlled prospective study conducted in Universiti Sains Malaysia. Powdered form of Malaysian Aloe vera leaf was extracted with 70% ethanol and aqueous via Soxhlet extraction method, after being evaporated using rotary evaporator to a thicker compound. The concentrated extract is then freeze dried to obtain powdered form which is diluted into establish five different concentrations of 50 g/ml, 25 g/ml, 12.5 g/ml, 6.25 g/ml and 3.125 g/ml. Five replicates were performed for five different concentration. Results were statistically analysed using one-way ANOVA and independant-t test. Sabaroud Dextrose Agar lawned with tested fungal isolates were inoculated with the extracts using well-diffusion method. Zone of inhibition was measured followed by minimum inhibitory concentration. Results: For Aspergillus niger, zone of inhibition for alcohol and aqueous extract were obtained for all concentrations except 3.125g/ml. As for Candida albicans, there were no zone of inhibition for both alcohol and aqueous extract of Aloe vera leaf. The minimum inhibitory concentration value of aqueous and alcohol extracts were 5.129g/ml and 4.35g/ml for Aspergillus niger and as for Candida albicans, it was not determined as no zone of inhibition was obtained. 2017 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/44982/1/Dr.%20Jeyasakthy%20Saniasiya-24%20pages.pdf Saniasiaya, Jeyasakthy (2017) Antifungal effect of Malaysian aloe vera leaf extract selected fungal species of pathologenic otomycosis in in-vitro culture medium. Masters thesis, Universiti Sains Malaysia.
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic RB Pathology
spellingShingle RB Pathology
Saniasiaya, Jeyasakthy
Antifungal effect of Malaysian aloe vera leaf extract selected fungal species of pathologenic otomycosis in in-vitro culture medium
description Introduction: Aloe barbadensis miller or Aloe vera has been used for therapeutic purposes since ancient time. Antifungal activity is amongst the medicinal properties owned by Aloe vera. This study proposes antifungal effect of Malaysian Aloe vera leaf extract on pathogenic otomycosis species, Aspergillus niger and Candida albicans. Methodology: This pilot study is a laboratory-controlled prospective study conducted in Universiti Sains Malaysia. Powdered form of Malaysian Aloe vera leaf was extracted with 70% ethanol and aqueous via Soxhlet extraction method, after being evaporated using rotary evaporator to a thicker compound. The concentrated extract is then freeze dried to obtain powdered form which is diluted into establish five different concentrations of 50 g/ml, 25 g/ml, 12.5 g/ml, 6.25 g/ml and 3.125 g/ml. Five replicates were performed for five different concentration. Results were statistically analysed using one-way ANOVA and independant-t test. Sabaroud Dextrose Agar lawned with tested fungal isolates were inoculated with the extracts using well-diffusion method. Zone of inhibition was measured followed by minimum inhibitory concentration. Results: For Aspergillus niger, zone of inhibition for alcohol and aqueous extract were obtained for all concentrations except 3.125g/ml. As for Candida albicans, there were no zone of inhibition for both alcohol and aqueous extract of Aloe vera leaf. The minimum inhibitory concentration value of aqueous and alcohol extracts were 5.129g/ml and 4.35g/ml for Aspergillus niger and as for Candida albicans, it was not determined as no zone of inhibition was obtained.
format Thesis
author Saniasiaya, Jeyasakthy
author_facet Saniasiaya, Jeyasakthy
author_sort Saniasiaya, Jeyasakthy
title Antifungal effect of Malaysian aloe vera leaf extract selected fungal species of pathologenic otomycosis in in-vitro culture medium
title_short Antifungal effect of Malaysian aloe vera leaf extract selected fungal species of pathologenic otomycosis in in-vitro culture medium
title_full Antifungal effect of Malaysian aloe vera leaf extract selected fungal species of pathologenic otomycosis in in-vitro culture medium
title_fullStr Antifungal effect of Malaysian aloe vera leaf extract selected fungal species of pathologenic otomycosis in in-vitro culture medium
title_full_unstemmed Antifungal effect of Malaysian aloe vera leaf extract selected fungal species of pathologenic otomycosis in in-vitro culture medium
title_sort antifungal effect of malaysian aloe vera leaf extract selected fungal species of pathologenic otomycosis in in-vitro culture medium
publishDate 2017
url http://eprints.usm.my/44982/1/Dr.%20Jeyasakthy%20Saniasiya-24%20pages.pdf
http://eprints.usm.my/44982/
_version_ 1681490161430429696
score 13.211869

Similar Items