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Increased Nociceptive Responses in Streptozotocin-Induced Diabetic Rats and the Related Expression of Spinal NR2B Subunit of N-Methyl-D-Aspartate Receptors

Background: This study investigated the role of NR2B in a modulated pain process in the painful diabetic neuropathy (PDN) rat using various pain stimuli. Methods: Thirty-two Sprague-Dawley male rats were randomly allocated into four groups (n=8): control, diabetes mellitus (DM) rats and diabetic ra...

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Main Authors: Ismail, Che Aishah Nazariah, Suppian, Rapeah, Aziz, Che Badariah Abd, Haris, Khalilah, Long, Idris
Format: Article
Language:English
Published: Korean Diabetes Association 2019
Subjects:
RC31-1245 Internal medicine
Online Access:http://eprints.usm.my/44427/1/dmj-43-222.pdf
http://eprints.usm.my/44427/
https://doi.org/10.4093/dmj.2018.0020
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spelling my.usm.eprints.44427 http://eprints.usm.my/44427/ Increased Nociceptive Responses in Streptozotocin-Induced Diabetic Rats and the Related Expression of Spinal NR2B Subunit of N-Methyl-D-Aspartate Receptors Ismail, Che Aishah Nazariah Suppian, Rapeah Aziz, Che Badariah Abd Haris, Khalilah Long, Idris RC31-1245 Internal medicine Background: This study investigated the role of NR2B in a modulated pain process in the painful diabetic neuropathy (PDN) rat using various pain stimuli. Methods: Thirty-two Sprague-Dawley male rats were randomly allocated into four groups (n=8): control, diabetes mellitus (DM) rats and diabetic rats treated with ifenprodil at a lower dose (0.5 μg/day) (I 0.5) or higher dose (1.0 μg/day) (I 1.0). DM was induced by a single injection of streptozotocin at 60 mg/kg on day 0 of experimentation. Diabetic status was assessed on day 3 of the experimentation. The responses on both tactile and thermal stimuli were assessed on day 0 (baseline), day 14 (pre-intervention), and day 22 (post-intervention). Ifenprodil was given intrathecally for 7 days from day 15 until day 21. On day 23, 5% formalin was injected into the rats’ hind paw and the nociceptive responses were recorded for 1 hour. The rats were sacrificed 72 hours post-formalin injection and an analysis of the spinal NR2B expression was performed. Results: DM rats showed a significant reduction in pain threshold in response to the tactile and thermal stimuli and higher nociceptive response during the formalin test accompanied by the higher expression of phosphorylated spinal NR2B in both sides of the spinal cord. Ifenprodil treatment for both doses showed anti-allodynic and anti-nociceptive effects with lower expression of phosphorylated and total spinal NR2B. Conclusion: We suggest that the pain process in the streptozotocin-induced diabetic rat that has been modulated is associated with the higher phosphorylation of the spinal NR2B expression in the development of PDN, which is similar to other models of neuropathic rats. Korean Diabetes Association 2019 Article PeerReviewed application/pdf en http://eprints.usm.my/44427/1/dmj-43-222.pdf Ismail, Che Aishah Nazariah and Suppian, Rapeah and Aziz, Che Badariah Abd and Haris, Khalilah and Long, Idris (2019) Increased Nociceptive Responses in Streptozotocin-Induced Diabetic Rats and the Related Expression of Spinal NR2B Subunit of N-Methyl-D-Aspartate Receptors. Diabetes and Metabolism Journal, 43 (2). pp. 222-235. ISSN 2233-6079 https://doi.org/10.4093/dmj.2018.0020
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic RC31-1245 Internal medicine
spellingShingle RC31-1245 Internal medicine
Ismail, Che Aishah Nazariah
Suppian, Rapeah
Aziz, Che Badariah Abd
Haris, Khalilah
Long, Idris
Increased Nociceptive Responses in Streptozotocin-Induced Diabetic Rats and the Related Expression of Spinal NR2B Subunit of N-Methyl-D-Aspartate Receptors
description Background: This study investigated the role of NR2B in a modulated pain process in the painful diabetic neuropathy (PDN) rat using various pain stimuli. Methods: Thirty-two Sprague-Dawley male rats were randomly allocated into four groups (n=8): control, diabetes mellitus (DM) rats and diabetic rats treated with ifenprodil at a lower dose (0.5 μg/day) (I 0.5) or higher dose (1.0 μg/day) (I 1.0). DM was induced by a single injection of streptozotocin at 60 mg/kg on day 0 of experimentation. Diabetic status was assessed on day 3 of the experimentation. The responses on both tactile and thermal stimuli were assessed on day 0 (baseline), day 14 (pre-intervention), and day 22 (post-intervention). Ifenprodil was given intrathecally for 7 days from day 15 until day 21. On day 23, 5% formalin was injected into the rats’ hind paw and the nociceptive responses were recorded for 1 hour. The rats were sacrificed 72 hours post-formalin injection and an analysis of the spinal NR2B expression was performed. Results: DM rats showed a significant reduction in pain threshold in response to the tactile and thermal stimuli and higher nociceptive response during the formalin test accompanied by the higher expression of phosphorylated spinal NR2B in both sides of the spinal cord. Ifenprodil treatment for both doses showed anti-allodynic and anti-nociceptive effects with lower expression of phosphorylated and total spinal NR2B. Conclusion: We suggest that the pain process in the streptozotocin-induced diabetic rat that has been modulated is associated with the higher phosphorylation of the spinal NR2B expression in the development of PDN, which is similar to other models of neuropathic rats.
format Article
author Ismail, Che Aishah Nazariah
Suppian, Rapeah
Aziz, Che Badariah Abd
Haris, Khalilah
Long, Idris
author_facet Ismail, Che Aishah Nazariah
Suppian, Rapeah
Aziz, Che Badariah Abd
Haris, Khalilah
Long, Idris
author_sort Ismail, Che Aishah Nazariah
title Increased Nociceptive Responses in Streptozotocin-Induced Diabetic Rats and the Related Expression of Spinal NR2B Subunit of N-Methyl-D-Aspartate Receptors
title_short Increased Nociceptive Responses in Streptozotocin-Induced Diabetic Rats and the Related Expression of Spinal NR2B Subunit of N-Methyl-D-Aspartate Receptors
title_full Increased Nociceptive Responses in Streptozotocin-Induced Diabetic Rats and the Related Expression of Spinal NR2B Subunit of N-Methyl-D-Aspartate Receptors
title_fullStr Increased Nociceptive Responses in Streptozotocin-Induced Diabetic Rats and the Related Expression of Spinal NR2B Subunit of N-Methyl-D-Aspartate Receptors
title_full_unstemmed Increased Nociceptive Responses in Streptozotocin-Induced Diabetic Rats and the Related Expression of Spinal NR2B Subunit of N-Methyl-D-Aspartate Receptors
title_sort increased nociceptive responses in streptozotocin-induced diabetic rats and the related expression of spinal nr2b subunit of n-methyl-d-aspartate receptors
publisher Korean Diabetes Association
publishDate 2019
url http://eprints.usm.my/44427/1/dmj-43-222.pdf
http://eprints.usm.my/44427/
https://doi.org/10.4093/dmj.2018.0020
_version_ 1643711001511591936
score 13.211869

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