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Perbandingan antigen larut (SA) Brugia malayi dan Brugia pahangi di dalam pengembangan sistem ELISA untuk diagnosis penyakit Filaria

Brugia malayi and Brugia pahangi adult worm soluble antigens were used in digoxigenin-sandwich ELISA to detect filarial specific anti-IgG4 and antiIgG2 antibodies in different groups of filarial patients, soil-transmitted helminth infected patients and in normal individuals. Both kinds of antigens s...

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Main Authors: Noordin, Rahmah, Abdullah, Khairul Anuar, Bakar, Afifi Sheikh Abu
Format: Conference or Workshop Item
Language:English
Published: 1999
Subjects:
RC Internal medicine
Online Access:http://eprints.usm.my/42390/1/GP...Perbandingan_Antigen_Larut_%28SA%29_Brugia_Malayi_Dan_Brugia_Pahangi_Di_Dalam_Pengembangan_Sistem_Elisa_Unuk_Diagnosis_Penyakit_Filaria...OCR..pdf
http://eprints.usm.my/42390/
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spelling my.usm.eprints.42390 http://eprints.usm.my/42390/ Perbandingan antigen larut (SA) Brugia malayi dan Brugia pahangi di dalam pengembangan sistem ELISA untuk diagnosis penyakit Filaria Noordin, Rahmah Abdullah, Khairul Anuar Bakar, Afifi Sheikh Abu RC Internal medicine Brugia malayi and Brugia pahangi adult worm soluble antigens were used in digoxigenin-sandwich ELISA to detect filarial specific anti-IgG4 and antiIgG2 antibodies in different groups of filarial patients, soil-transmitted helminth infected patients and in normal individuals. Both kinds of antigens showed positive IgG4 responses with all sera of microfilaraemic patients. However, ELISA using B. pahangi antigen also showed positive ELISA readings with some sera of patients with soil-transmitted helminth infections and some sera of normal individuals. On the other hand no such cross-reactivities were demonstrated when B. malayi was used as the antigen. In the analysis of anti-filarial IgG2 antibody responses, all chronic elephantiasis sera gave positive readings in ELlS As that employed B. malayi as the antigen; and no cross-reactivity with other helminthic infections were observed. In comparison, only some sera of chronic elephantiasis patients were positive for antifilaral IgG2 antibodies when B. pahangi was used as the antigen. Furthermore, with the use of B. pahangi antigen coated plates, some sera from soil-transmitted helminth infected patients and from normal individuals also showed positive titres of antifilarial IgG2 antibodies. This study demonstrated that B. pahangi soluble adult worm antigen is not a suitable substitute for B. malayi soluble adult worm antigen in the diagnosis of brugian filariasis by ELISA. 1999 Conference or Workshop Item NonPeerReviewed application/pdf en http://eprints.usm.my/42390/1/GP...Perbandingan_Antigen_Larut_%28SA%29_Brugia_Malayi_Dan_Brugia_Pahangi_Di_Dalam_Pengembangan_Sistem_Elisa_Unuk_Diagnosis_Penyakit_Filaria...OCR..pdf Noordin, Rahmah and Abdullah, Khairul Anuar and Bakar, Afifi Sheikh Abu (1999) Perbandingan antigen larut (SA) Brugia malayi dan Brugia pahangi di dalam pengembangan sistem ELISA untuk diagnosis penyakit Filaria. In: Perbandingan antigen larut (SA) Brugia malayi dan Brugia pahangi di dalam pengembangan sistem ELISA untuk diagnosis penyakit Filaria. (Submitted)
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic RC Internal medicine
spellingShingle RC Internal medicine
Noordin, Rahmah
Abdullah, Khairul Anuar
Bakar, Afifi Sheikh Abu
Perbandingan antigen larut (SA) Brugia malayi dan Brugia pahangi di dalam pengembangan sistem ELISA untuk diagnosis penyakit Filaria
description Brugia malayi and Brugia pahangi adult worm soluble antigens were used in digoxigenin-sandwich ELISA to detect filarial specific anti-IgG4 and antiIgG2 antibodies in different groups of filarial patients, soil-transmitted helminth infected patients and in normal individuals. Both kinds of antigens showed positive IgG4 responses with all sera of microfilaraemic patients. However, ELISA using B. pahangi antigen also showed positive ELISA readings with some sera of patients with soil-transmitted helminth infections and some sera of normal individuals. On the other hand no such cross-reactivities were demonstrated when B. malayi was used as the antigen. In the analysis of anti-filarial IgG2 antibody responses, all chronic elephantiasis sera gave positive readings in ELlS As that employed B. malayi as the antigen; and no cross-reactivity with other helminthic infections were observed. In comparison, only some sera of chronic elephantiasis patients were positive for antifilaral IgG2 antibodies when B. pahangi was used as the antigen. Furthermore, with the use of B. pahangi antigen coated plates, some sera from soil-transmitted helminth infected patients and from normal individuals also showed positive titres of antifilarial IgG2 antibodies. This study demonstrated that B. pahangi soluble adult worm antigen is not a suitable substitute for B. malayi soluble adult worm antigen in the diagnosis of brugian filariasis by ELISA.
format Conference or Workshop Item
author Noordin, Rahmah
Abdullah, Khairul Anuar
Bakar, Afifi Sheikh Abu
author_facet Noordin, Rahmah
Abdullah, Khairul Anuar
Bakar, Afifi Sheikh Abu
author_sort Noordin, Rahmah
title Perbandingan antigen larut (SA) Brugia malayi dan Brugia pahangi di dalam pengembangan sistem ELISA untuk diagnosis penyakit Filaria
title_short Perbandingan antigen larut (SA) Brugia malayi dan Brugia pahangi di dalam pengembangan sistem ELISA untuk diagnosis penyakit Filaria
title_full Perbandingan antigen larut (SA) Brugia malayi dan Brugia pahangi di dalam pengembangan sistem ELISA untuk diagnosis penyakit Filaria
title_fullStr Perbandingan antigen larut (SA) Brugia malayi dan Brugia pahangi di dalam pengembangan sistem ELISA untuk diagnosis penyakit Filaria
title_full_unstemmed Perbandingan antigen larut (SA) Brugia malayi dan Brugia pahangi di dalam pengembangan sistem ELISA untuk diagnosis penyakit Filaria
title_sort perbandingan antigen larut (sa) brugia malayi dan brugia pahangi di dalam pengembangan sistem elisa untuk diagnosis penyakit filaria
publishDate 1999
url http://eprints.usm.my/42390/1/GP...Perbandingan_Antigen_Larut_%28SA%29_Brugia_Malayi_Dan_Brugia_Pahangi_Di_Dalam_Pengembangan_Sistem_Elisa_Unuk_Diagnosis_Penyakit_Filaria...OCR..pdf
http://eprints.usm.my/42390/
_version_ 1662755722055647232
score 13.211869

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