Mengenalpasti fenotip apolipoprorein E dengan menggunakan teknik fokus isoelektrik bagi kes-kes hiperlipidemia di Kelantan
Patients plasma samples (n=250) were screened for cholesterol and triglyceride content by enzymatic analyses performed on an automated chemistry analyzer using standard kits. Lipoprotein electrophoresis was performed on thin slab or pre-casted tube gels.Unrelated plasma were selected (n=24) and stud...
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| Main Authors: | , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
1999
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| Subjects: | |
| Online Access: | http://eprints.usm.my/42376/1/GP...Mengenalpasti_Fenotip_Apolipoprotein_E_Dengan_Menggunakan_Teknik_Fokus_Isoelektrik_Bagi_Kes-Kes_Hiperlipidemia_Di_Kelantan...OCR.pdf http://eprints.usm.my/42376/ |
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| Summary: | Patients plasma samples (n=250) were screened for cholesterol and triglyceride content by enzymatic analyses performed on an automated chemistry analyzer using standard kits. Lipoprotein electrophoresis was performed on thin slab or pre-casted tube gels.Unrelated plasma were selected (n=24) and studied using both gel methods. One subject with a possible apolipoprotein E-2/2 phenotype was detected by the slab gel method. Another 11 subjects with unusual lipoprotein banding patterns and possible altered apolipoprotein E phenotypes were detected by the tube gel method. VLDL were isolated by a micromethod developed on an ultracentrifuge. Apolipoprotein E phenotype was confirmed by flat bed isoelectrtc focussing (IEF) of delipidated VLDL proteins on commercial and homemade IEF gels. |
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