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Autophagic Cell Death Is Induced by Acetone and Ethyl Acetate Extracts fromEupatorium odoratum InVitro : Effects onMCF-7 and Vero Cell Lines

Eupatorium odoratum (EO) contains many biologically active compounds, the anticancer effects of which are not well documented. This study evaluates the cytotoxic effects and mechanism of action of EO extracts on MCF-7 and Vero cell lines. Evaluation of the cytotoxic activity using MTT assay, morph...

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Main Authors: Harun, Faizah, Syed Sahil Jamalullail, Syed Mohsin, Khoo, Boon Yin, Othman, Zulkhairi, Tilwari, Anita, Balaram, Prabha
Format: Article
Language:English
Published: Hindawi Publishing Corporation 2012
Subjects:
R735-854 Medical education. Medical schools. Research
Online Access:http://eprints.usm.my/38380/1/Autophagic_Cell_Death_Is_Induced_by_Acetone_and_Ethyl_Acetate_Extracts_from_Eupatorium_odoratum_In_Vitro.pdf
http://eprints.usm.my/38380/
https://doi.org/10.1100/2012/439479
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spelling my.usm.eprints.38380 http://eprints.usm.my/38380/ Autophagic Cell Death Is Induced by Acetone and Ethyl Acetate Extracts fromEupatorium odoratum InVitro : Effects onMCF-7 and Vero Cell Lines Harun, Faizah Syed Sahil Jamalullail, Syed Mohsin Khoo, Boon Yin Othman, Zulkhairi Tilwari, Anita Balaram, Prabha R735-854 Medical education. Medical schools. Research Eupatorium odoratum (EO) contains many biologically active compounds, the anticancer effects of which are not well documented. This study evaluates the cytotoxic effects and mechanism of action of EO extracts on MCF-7 and Vero cell lines. Evaluation of the cytotoxic activity using MTT assay, morphological alterations, and apoptosis were carried out. Autophagy was evaluated by LC3-A protein expression. Cytotoxic activity,membrane blebbing and ballooning at 24 hours, replacement bymass vacuolation, and double membrane vesicles mimicking autophagy and cell death were observed in the cancer cells. No apoptosis was observed by DNA fragmentation assay. Overexpression of LC3-A protein indicated autophagic cell death. Cell cycle analysis showed G0 and G2/M arrest. The Vero cells did not show significant cell death at concentrations <100 μg/mL. These results thus suggest that acetone and ethyl acetate extracts of EO induce cell death through induction of autophagy and hold potential for development as potential anticancer drugs. Hindawi Publishing Corporation 2012 Article PeerReviewed application/pdf en http://eprints.usm.my/38380/1/Autophagic_Cell_Death_Is_Induced_by_Acetone_and_Ethyl_Acetate_Extracts_from_Eupatorium_odoratum_In_Vitro.pdf Harun, Faizah and Syed Sahil Jamalullail, Syed Mohsin and Khoo, Boon Yin and Othman, Zulkhairi and Tilwari, Anita and Balaram, Prabha (2012) Autophagic Cell Death Is Induced by Acetone and Ethyl Acetate Extracts fromEupatorium odoratum InVitro : Effects onMCF-7 and Vero Cell Lines. Scientific World Journal, 2012 (439479). pp. 1-9. ISSN 2356-6140 https://doi.org/10.1100/2012/439479
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic R735-854 Medical education. Medical schools. Research
spellingShingle R735-854 Medical education. Medical schools. Research
Harun, Faizah
Syed Sahil Jamalullail, Syed Mohsin
Khoo, Boon Yin
Othman, Zulkhairi
Tilwari, Anita
Balaram, Prabha
Autophagic Cell Death Is Induced by Acetone and Ethyl Acetate Extracts fromEupatorium odoratum InVitro : Effects onMCF-7 and Vero Cell Lines
description Eupatorium odoratum (EO) contains many biologically active compounds, the anticancer effects of which are not well documented. This study evaluates the cytotoxic effects and mechanism of action of EO extracts on MCF-7 and Vero cell lines. Evaluation of the cytotoxic activity using MTT assay, morphological alterations, and apoptosis were carried out. Autophagy was evaluated by LC3-A protein expression. Cytotoxic activity,membrane blebbing and ballooning at 24 hours, replacement bymass vacuolation, and double membrane vesicles mimicking autophagy and cell death were observed in the cancer cells. No apoptosis was observed by DNA fragmentation assay. Overexpression of LC3-A protein indicated autophagic cell death. Cell cycle analysis showed G0 and G2/M arrest. The Vero cells did not show significant cell death at concentrations <100 μg/mL. These results thus suggest that acetone and ethyl acetate extracts of EO induce cell death through induction of autophagy and hold potential for development as potential anticancer drugs.
format Article
author Harun, Faizah
Syed Sahil Jamalullail, Syed Mohsin
Khoo, Boon Yin
Othman, Zulkhairi
Tilwari, Anita
Balaram, Prabha
author_facet Harun, Faizah
Syed Sahil Jamalullail, Syed Mohsin
Khoo, Boon Yin
Othman, Zulkhairi
Tilwari, Anita
Balaram, Prabha
author_sort Harun, Faizah
title Autophagic Cell Death Is Induced by Acetone and Ethyl Acetate Extracts fromEupatorium odoratum InVitro : Effects onMCF-7 and Vero Cell Lines
title_short Autophagic Cell Death Is Induced by Acetone and Ethyl Acetate Extracts fromEupatorium odoratum InVitro : Effects onMCF-7 and Vero Cell Lines
title_full Autophagic Cell Death Is Induced by Acetone and Ethyl Acetate Extracts fromEupatorium odoratum InVitro : Effects onMCF-7 and Vero Cell Lines
title_fullStr Autophagic Cell Death Is Induced by Acetone and Ethyl Acetate Extracts fromEupatorium odoratum InVitro : Effects onMCF-7 and Vero Cell Lines
title_full_unstemmed Autophagic Cell Death Is Induced by Acetone and Ethyl Acetate Extracts fromEupatorium odoratum InVitro : Effects onMCF-7 and Vero Cell Lines
title_sort autophagic cell death is induced by acetone and ethyl acetate extracts fromeupatorium odoratum invitro : effects onmcf-7 and vero cell lines
publisher Hindawi Publishing Corporation
publishDate 2012
url http://eprints.usm.my/38380/1/Autophagic_Cell_Death_Is_Induced_by_Acetone_and_Ethyl_Acetate_Extracts_from_Eupatorium_odoratum_In_Vitro.pdf
http://eprints.usm.my/38380/
https://doi.org/10.1100/2012/439479
_version_ 1643709340668919808
score 13.211869

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