Association of anti-CLIC2 and anti-HMGB1 autoantibodies with higher disease activity in systemic lupus erythematosus patients
Systemic lupus erythematosus (SLE) is a systemic autoimmune disease characterized by numerous autoantibodies. In this study, we investigated the presence of anti-chloride intracellular channel 2 (anti-CLIC2) and anti-high mobility group box 1 (anti-HMGB1) autoantibodies in SLE patients (n = 43) ve...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Medknow Publications
2017
|
| Subjects: | |
| Online Access: | http://eprints.usm.my/38371/1/Association_of_anti-CLIC2_and_anti-HMGB1_autoantibodies.pdf http://eprints.usm.my/38371/ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5664871/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Systemic lupus erythematosus (SLE) is a systemic autoimmune disease characterized by
numerous autoantibodies. In this study, we investigated the presence of anti-chloride intracellular channel 2
(anti-CLIC2) and anti-high mobility group box 1 (anti-HMGB1) autoantibodies in SLE patients (n = 43) versus
healthy controls ([HCs] n = 43), and their association with serological parameters (antinuclear antibody [ANA],
anti-double-stranded DNA [anti-dsDNA], and C-reactive protein [CRP]) and disease activity using Systemic
Lupus Erythematosus Disease Activity Index (SLEDAI) score (active or inactive). Settings and Design: Case–
control study at Rheumatology Clinic of Universiti Sains Malaysia Hospital. Subjects and Methods: The sera of
SLE patients and HCs were tested for the presence of anti-CLIC2 and anti-HMGB1 autoantibodies using human
recombinant proteins and ELISA methodologies. Other serological parameters were evaluated according to
routine procedures, and patients' demographic and clinical data were obtained. Statistical Analysis: Mann–
Whitney U-test, Chi-square test, Fisher's exact test, and receiver operating characteristic analysis. Results:
Anti-CLIC2 autoantibody levels were significantly higher in SLE patients compared to HCs (P = 0.0035), whereas
anti-HMGB1 autoantibody levels were not significantly elevated (P = 0.7702). Anti-CLIC2 and anti-HMGB1
autoantibody levels were not associated with ANA pattern, anti-dsDNA, and CRP. Interestingly, SLEDAI score
(≥6) was associated with anti-CLIC2 (P = 0.0046) and with anti-HMGB1 (P = 0.0091) autoantibody levels.
Conclusion: Our findings support the potential of using anti-CLIC2 autoantibodies as a novel biomarker for SLE
patients. Both anti-CLIC2 and anti-HMGB1 autoantibody levels demonstrated potential in monitoring SLE disease
activity. |
|---|