Negative effects of nicotine on bone-resorbing cytokines and bone histomorphometric parameters in male rats
The effects of nicotine administration on boneresorbing cytokines, cotinine, and bone histomorphometric parameters were studied in 21 Sprague–Dawley male rats. Rats aged 3 months and weighing 250–300 g were divided into three groups. Group 1 was the baseline control (BC), which was killed witho...
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https://doi.org/10.1007/s00774-006-0733-9
2007
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my.usm.eprints.35763 http://eprints.usm.my/35763/ Negative effects of nicotine on bone-resorbing cytokines and bone histomorphometric parameters in male rats Hapidin, Hermizi Othman, Faizah Soelaiman, Ima-Nirwana Douglas A, Luke Mohamed, Norazlina RA0421 Public health. Hygiene. Preventive Medicine The effects of nicotine administration on boneresorbing cytokines, cotinine, and bone histomorphometric parameters were studied in 21 Sprague–Dawley male rats. Rats aged 3 months and weighing 250–300 g were divided into three groups. Group 1 was the baseline control (BC), which was killed without treatment. The other two groups were the control group (C) and the nicotine-treated group (N). The N group was treated with nicotine 7 mg/kg body weight and the C group was treated with normal saline only. Treatment was given by intraperitoneal injection for 6 days/week for 4 months. The rats were injected intraperitoneally with calcein 20 mg/kg body weight at day 9 and day 2 before they were killed. ELISA test kits were used to measure the serum interleukin-1 (IL-1), interleukin-6 (IL-6), and cotinine (a metabolite of nicotine) levels at the beginning of the study and upon completion of the study. Histomorphometric analysis was done on the metaphyseal region of the trabecular bone of the left femur by using an image analyzer. Biochemical analysis revealed that nicotine treatment for 4 months signifi cantly increased the serum IL-1, IL-6, and cotinine levels as compared to pretreatment levels. In addition, the serum cotinine level was signifi cantly higher in the N group than in the C group after 4 months treatment. Histomorphometric analysis showed that nicotine signifi cantly decreased the trabecular bone volume (BV/TV), trabecular thickness (Tb.Th), double-labeled surface (dLS/BS), mineralizing surface (MS/BS), mineral appositional rate (MAR), and bone formation rate (BFR/BS), while causing an increase in the single-labeled surface (sLS/BS), osteoclast surface (Oc.S/BS), and eroded surface (ES/BS) as compared to the BC and C groups. In conclusion, treatment with nicotine 7 mg/kg for 4 months was detrimental to bone by causing an increase in the bone resorbing cytokines and cotinine levels. Nicotine also exerted negative effects on the dynamic trabecular histomorphometric parameters. https://doi.org/10.1007/s00774-006-0733-9 2007 Article PeerReviewed Hapidin, Hermizi and Othman, Faizah and Soelaiman, Ima-Nirwana and Douglas A, Luke and Mohamed, Norazlina (2007) Negative effects of nicotine on bone-resorbing cytokines and bone histomorphometric parameters in male rats. J Bone Miner Metab, 25. pp. 93-98. |
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RA0421 Public health. Hygiene. Preventive Medicine Hapidin, Hermizi Othman, Faizah Soelaiman, Ima-Nirwana Douglas A, Luke Mohamed, Norazlina Negative effects of nicotine on bone-resorbing cytokines and bone histomorphometric parameters in male rats |
| description |
The effects of nicotine administration on boneresorbing
cytokines, cotinine, and bone histomorphometric
parameters were studied in 21 Sprague–Dawley
male rats. Rats aged 3 months and weighing 250–300 g
were divided into three groups. Group 1 was the baseline
control (BC), which was killed without treatment. The
other two groups were the control group (C) and the
nicotine-treated group (N). The N group was treated with
nicotine 7 mg/kg body weight and the C group was treated
with normal saline only. Treatment was given by intraperitoneal
injection for 6 days/week for 4 months. The rats
were injected intraperitoneally with calcein 20 mg/kg body
weight at day 9 and day 2 before they were killed. ELISA
test kits were used to measure the serum interleukin-1
(IL-1), interleukin-6 (IL-6), and cotinine (a metabolite of
nicotine) levels at the beginning of the study and upon
completion of the study. Histomorphometric analysis was
done on the metaphyseal region of the trabecular bone of
the left femur by using an image analyzer. Biochemical
analysis revealed that nicotine treatment for 4 months signifi
cantly increased the serum IL-1, IL-6, and cotinine
levels as compared to pretreatment levels. In addition, the
serum cotinine level was signifi cantly higher in the N
group than in the C group after 4 months treatment. Histomorphometric
analysis showed that nicotine signifi cantly
decreased the trabecular bone volume (BV/TV), trabecular
thickness (Tb.Th), double-labeled surface (dLS/BS),
mineralizing surface (MS/BS), mineral appositional rate
(MAR), and bone formation rate (BFR/BS), while causing
an increase in the single-labeled surface (sLS/BS), osteoclast
surface (Oc.S/BS), and eroded surface (ES/BS) as
compared to the BC and C groups. In conclusion, treatment
with nicotine 7 mg/kg for 4 months was detrimental
to bone by causing an increase in the bone resorbing
cytokines and cotinine levels. Nicotine also exerted negative
effects on the dynamic trabecular histomorphometric
parameters. |
| format |
Article |
| author |
Hapidin, Hermizi Othman, Faizah Soelaiman, Ima-Nirwana Douglas A, Luke Mohamed, Norazlina |
| author_facet |
Hapidin, Hermizi Othman, Faizah Soelaiman, Ima-Nirwana Douglas A, Luke Mohamed, Norazlina |
| author_sort |
Hapidin, Hermizi |
| title |
Negative effects of nicotine on bone-resorbing cytokines and bone histomorphometric parameters in male rats |
| title_short |
Negative effects of nicotine on bone-resorbing cytokines and bone histomorphometric parameters in male rats |
| title_full |
Negative effects of nicotine on bone-resorbing cytokines and bone histomorphometric parameters in male rats |
| title_fullStr |
Negative effects of nicotine on bone-resorbing cytokines and bone histomorphometric parameters in male rats |
| title_full_unstemmed |
Negative effects of nicotine on bone-resorbing cytokines and bone histomorphometric parameters in male rats |
| title_sort |
negative effects of nicotine on bone-resorbing cytokines and bone histomorphometric parameters in male rats |
| publisher |
https://doi.org/10.1007/s00774-006-0733-9 |
| publishDate |
2007 |
| url |
http://eprints.usm.my/35763/ |
| _version_ |
1643708589530939392 |
| score |
13.211869 |