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Characterization Of The Depolymerizing Activity Of Commercial Lipases And Animal Organ Extracts Using A Simple Polyhydroxyalkanoate-Based Microassay

Lipase ialah enzim yang digunakan dalam pelbagai aplikasi dan aktivitinya perlu diuji sebelum aplikasi. Namun begitu, kebanyakkan asai aktiviti lipase memerlukan bahan kimia toksik dan persediaan yang rumit. Oleh itu, poli(3-hidroksibutirat-ko-4-hidroksibutirat) [P(3HB-ko-4HB)] digunakan sebagai sub...

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Main Author: Mok, Pei Shze
Format: Thesis
Language:English
Published: 2015
Subjects:
QH1-278.5 Natural history (General)
Online Access:http://eprints.usm.my/31939/1/Mok_Pei_Shze.pdf
http://eprints.usm.my/31939/
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spelling my.usm.eprints.31939 http://eprints.usm.my/31939/ Characterization Of The Depolymerizing Activity Of Commercial Lipases And Animal Organ Extracts Using A Simple Polyhydroxyalkanoate-Based Microassay Mok, Pei Shze QH1-278.5 Natural history (General) Lipase ialah enzim yang digunakan dalam pelbagai aplikasi dan aktivitinya perlu diuji sebelum aplikasi. Namun begitu, kebanyakkan asai aktiviti lipase memerlukan bahan kimia toksik dan persediaan yang rumit. Oleh itu, poli(3-hidroksibutirat-ko-4-hidroksibutirat) [P(3HB-ko-4HB)] digunakan sebagai substrat pepejal kerana ia dapat diuraikan oleh lipase. Substrat ini boleh diuraikan secara semula jadi dan tidak memerlukan bahan kimia yang bertoksik. Dalam kajian ini, 2.0 ± 0 g/L sel mengandungi 28 ± 2 % of PHA dengan 92 ± 1 mol % of 4HB dalam PHA telah dibiosintesis. Kestabilan filem P(3HB-ko-4HB) dinilai dengan menyimpan filem tersebut di bawah keadaan yang berbeza iaitu di ketuhar vakum, balang pengering dan meja makmal selama 15 bulan. Filem tersebut paling stabil dalam balang pengering yang mengandungi gel silika. Aktiviti depolimerasi lipase komersial dan ekstrak organ haiwan telah diuji secara mikroasai dengan menggunakan P(3HB-ko-4HB) sebagai substrat. Lipase is an enzyme that is widely used in different applications and it is important to screen its activity before application. However, most of the available lipase activity assays require toxic chemicals and tedious preparation. Therefore, a solid substrate known as poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] is used and it can be degraded by lipases. This substrate is biodegradable and does not require toxic chemical. In this study, 2.0 ± 0 g/L of cells consisting 28 ± 2 % of PHA with 92 ± 1 mol % of 4HB were produced through biosynthesis. The stability of P(3HB-co-4HB) films was evaluated by storing them in different locations, in vacuum oven, desiccators and bench for 15 months. The film was found to be the most stable in desiccators containing silica gel. The depolymerizing activity of different known commercial lipases and animal organ extracts using P(3HB-co-92 mol% 4HB) as substrate was investigated via microassay. 2015-09 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/31939/1/Mok_Pei_Shze.pdf Mok, Pei Shze (2015) Characterization Of The Depolymerizing Activity Of Commercial Lipases And Animal Organ Extracts Using A Simple Polyhydroxyalkanoate-Based Microassay. Masters thesis, Universiti Sains Malaysia.
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic QH1-278.5 Natural history (General)
spellingShingle QH1-278.5 Natural history (General)
Mok, Pei Shze
Characterization Of The Depolymerizing Activity Of Commercial Lipases And Animal Organ Extracts Using A Simple Polyhydroxyalkanoate-Based Microassay
description Lipase ialah enzim yang digunakan dalam pelbagai aplikasi dan aktivitinya perlu diuji sebelum aplikasi. Namun begitu, kebanyakkan asai aktiviti lipase memerlukan bahan kimia toksik dan persediaan yang rumit. Oleh itu, poli(3-hidroksibutirat-ko-4-hidroksibutirat) [P(3HB-ko-4HB)] digunakan sebagai substrat pepejal kerana ia dapat diuraikan oleh lipase. Substrat ini boleh diuraikan secara semula jadi dan tidak memerlukan bahan kimia yang bertoksik. Dalam kajian ini, 2.0 ± 0 g/L sel mengandungi 28 ± 2 % of PHA dengan 92 ± 1 mol % of 4HB dalam PHA telah dibiosintesis. Kestabilan filem P(3HB-ko-4HB) dinilai dengan menyimpan filem tersebut di bawah keadaan yang berbeza iaitu di ketuhar vakum, balang pengering dan meja makmal selama 15 bulan. Filem tersebut paling stabil dalam balang pengering yang mengandungi gel silika. Aktiviti depolimerasi lipase komersial dan ekstrak organ haiwan telah diuji secara mikroasai dengan menggunakan P(3HB-ko-4HB) sebagai substrat. Lipase is an enzyme that is widely used in different applications and it is important to screen its activity before application. However, most of the available lipase activity assays require toxic chemicals and tedious preparation. Therefore, a solid substrate known as poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] is used and it can be degraded by lipases. This substrate is biodegradable and does not require toxic chemical. In this study, 2.0 ± 0 g/L of cells consisting 28 ± 2 % of PHA with 92 ± 1 mol % of 4HB were produced through biosynthesis. The stability of P(3HB-co-4HB) films was evaluated by storing them in different locations, in vacuum oven, desiccators and bench for 15 months. The film was found to be the most stable in desiccators containing silica gel. The depolymerizing activity of different known commercial lipases and animal organ extracts using P(3HB-co-92 mol% 4HB) as substrate was investigated via microassay.
format Thesis
author Mok, Pei Shze
author_facet Mok, Pei Shze
author_sort Mok, Pei Shze
title Characterization Of The Depolymerizing Activity Of Commercial Lipases And Animal Organ Extracts Using A Simple Polyhydroxyalkanoate-Based Microassay
title_short Characterization Of The Depolymerizing Activity Of Commercial Lipases And Animal Organ Extracts Using A Simple Polyhydroxyalkanoate-Based Microassay
title_full Characterization Of The Depolymerizing Activity Of Commercial Lipases And Animal Organ Extracts Using A Simple Polyhydroxyalkanoate-Based Microassay
title_fullStr Characterization Of The Depolymerizing Activity Of Commercial Lipases And Animal Organ Extracts Using A Simple Polyhydroxyalkanoate-Based Microassay
title_full_unstemmed Characterization Of The Depolymerizing Activity Of Commercial Lipases And Animal Organ Extracts Using A Simple Polyhydroxyalkanoate-Based Microassay
title_sort characterization of the depolymerizing activity of commercial lipases and animal organ extracts using a simple polyhydroxyalkanoate-based microassay
publishDate 2015
url http://eprints.usm.my/31939/1/Mok_Pei_Shze.pdf
http://eprints.usm.my/31939/
_version_ 1643707518194548736
score 13.211869

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