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Sortase– Mediated Transpeptidation: The Application Of Sortase A As A Tool For Site- Specific Modification Of Recombinant Antibodies

Diagnostik ‘point-of-care’ (POC) untuk kawasan terpencil telah menjadi cabaran besar untuk memerangi penyakit berjangkit di seluruh dunia. Pembangunan kaedah untuk membantu proses pembangunan diagnostik POC baru adalah penting untuk memperbaiki senario penjagaan kesihatan di seluruh dunia. Format ut...

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Main Author: Ismail, Nur Faezee
Format: Thesis
Language:English
Published: 2016
Subjects:
R735-854 Medical education. Medical schools. Research
Online Access:http://eprints.usm.my/31823/1/NUR_FAEZEE_BINTI_ISMAIL_24%28NN%29.pdf
http://eprints.usm.my/31823/
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spelling my.usm.eprints.31823 http://eprints.usm.my/31823/ Sortase– Mediated Transpeptidation: The Application Of Sortase A As A Tool For Site- Specific Modification Of Recombinant Antibodies Ismail, Nur Faezee R735-854 Medical education. Medical schools. Research Diagnostik ‘point-of-care’ (POC) untuk kawasan terpencil telah menjadi cabaran besar untuk memerangi penyakit berjangkit di seluruh dunia. Pembangunan kaedah untuk membantu proses pembangunan diagnostik POC baru adalah penting untuk memperbaiki senario penjagaan kesihatan di seluruh dunia. Format utama POC asai adalah pengesanan interaksi antibodi- antigen . Untuk mencapai matlamat ini, asai yang mampu melaporkan interaksi antara antibodi dan antigen adalah diperlukan. Kebiasaannya. asai dibangunkan dengan merangkaikan antibodi-antibodi kepada satu pelapor yang spesifik seperti enzim atau protein. Projek ini menerangkan penggunaan transpeptidasi berantarakan sortase sebagai satu cara untuk merangkaikan antibodi rekombinan untuk sepasang protein pelapor. Enzim Sortase adalah unik dengan mengenali motif yang mempunyai urutan yang khusus untuk membenarkan tidak balas rangkaian protein-protein secara setempat. Point-of-care (POC) diagnostics for resource deprived regions remains a major challenge to combat infectious diseases worldwide. Development of new methods to aid the development process of new POC diagnostics is essential to improve the healthcare scenario worldwide. The major format of POC assay is the detection of antibody-antigen interaction. To achieve this, an assay is required to be capable of reporting the interaction between the antibody and antigen. In most common assays, these detection are achieved by conjugating antibodies to specific reporter molecules such as enzymes or proteins. The work describes the application of sortase mediated transpeptidation as a means to conjugate recombinant antibodies to a pair of reporter proteins. 2016-03 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/31823/1/NUR_FAEZEE_BINTI_ISMAIL_24%28NN%29.pdf Ismail, Nur Faezee (2016) Sortase– Mediated Transpeptidation: The Application Of Sortase A As A Tool For Site- Specific Modification Of Recombinant Antibodies. Masters thesis, Universiti Sains Malaysia.
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic R735-854 Medical education. Medical schools. Research
spellingShingle R735-854 Medical education. Medical schools. Research
Ismail, Nur Faezee
Sortase– Mediated Transpeptidation: The Application Of Sortase A As A Tool For Site- Specific Modification Of Recombinant Antibodies
description Diagnostik ‘point-of-care’ (POC) untuk kawasan terpencil telah menjadi cabaran besar untuk memerangi penyakit berjangkit di seluruh dunia. Pembangunan kaedah untuk membantu proses pembangunan diagnostik POC baru adalah penting untuk memperbaiki senario penjagaan kesihatan di seluruh dunia. Format utama POC asai adalah pengesanan interaksi antibodi- antigen . Untuk mencapai matlamat ini, asai yang mampu melaporkan interaksi antara antibodi dan antigen adalah diperlukan. Kebiasaannya. asai dibangunkan dengan merangkaikan antibodi-antibodi kepada satu pelapor yang spesifik seperti enzim atau protein. Projek ini menerangkan penggunaan transpeptidasi berantarakan sortase sebagai satu cara untuk merangkaikan antibodi rekombinan untuk sepasang protein pelapor. Enzim Sortase adalah unik dengan mengenali motif yang mempunyai urutan yang khusus untuk membenarkan tidak balas rangkaian protein-protein secara setempat. Point-of-care (POC) diagnostics for resource deprived regions remains a major challenge to combat infectious diseases worldwide. Development of new methods to aid the development process of new POC diagnostics is essential to improve the healthcare scenario worldwide. The major format of POC assay is the detection of antibody-antigen interaction. To achieve this, an assay is required to be capable of reporting the interaction between the antibody and antigen. In most common assays, these detection are achieved by conjugating antibodies to specific reporter molecules such as enzymes or proteins. The work describes the application of sortase mediated transpeptidation as a means to conjugate recombinant antibodies to a pair of reporter proteins.
format Thesis
author Ismail, Nur Faezee
author_facet Ismail, Nur Faezee
author_sort Ismail, Nur Faezee
title Sortase– Mediated Transpeptidation: The Application Of Sortase A As A Tool For Site- Specific Modification Of Recombinant Antibodies
title_short Sortase– Mediated Transpeptidation: The Application Of Sortase A As A Tool For Site- Specific Modification Of Recombinant Antibodies
title_full Sortase– Mediated Transpeptidation: The Application Of Sortase A As A Tool For Site- Specific Modification Of Recombinant Antibodies
title_fullStr Sortase– Mediated Transpeptidation: The Application Of Sortase A As A Tool For Site- Specific Modification Of Recombinant Antibodies
title_full_unstemmed Sortase– Mediated Transpeptidation: The Application Of Sortase A As A Tool For Site- Specific Modification Of Recombinant Antibodies
title_sort sortase– mediated transpeptidation: the application of sortase a as a tool for site- specific modification of recombinant antibodies
publishDate 2016
url http://eprints.usm.my/31823/1/NUR_FAEZEE_BINTI_ISMAIL_24%28NN%29.pdf
http://eprints.usm.my/31823/
_version_ 1643707489821130752
score 13.211869

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