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Effects Of Galactose And Fructose On The Expression Of Isocitrate Lyase (Icl1) Enzyme In Candida Albicans

Sifat kepatogenan dalam C. albicans bergantung kepada atribut kecergasan dan juga faktor-faktor virulen yang lain. Ini termasuk sifat kental terhadap tindak balas tekanan dan penyesuaian metabolik. C. albicans boleh menduduki kebanyakkan persekitaran dalam ruang badan manusia yang mengandungi kepelb...

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Main Author: Ting , Seng Yeat
Format: Thesis
Language:English
Published: 2016
Subjects:
RK1-715 Dentistry
Online Access:http://eprints.usm.my/31499/1/TING_SENG_YEAT_24.pdf
http://eprints.usm.my/31499/
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spelling my.usm.eprints.31499 http://eprints.usm.my/31499/ Effects Of Galactose And Fructose On The Expression Of Isocitrate Lyase (Icl1) Enzyme In Candida Albicans Ting , Seng Yeat RK1-715 Dentistry Sifat kepatogenan dalam C. albicans bergantung kepada atribut kecergasan dan juga faktor-faktor virulen yang lain. Ini termasuk sifat kental terhadap tindak balas tekanan dan penyesuaian metabolik. C. albicans boleh menduduki kebanyakkan persekitaran dalam ruang badan manusia yang mengandungi kepelbagaian sumber karbon yang berbeza. Asimilasi sumber karbon adalah penting untuk pertumbuhan dan juga untuk meneruskan jangkitan. Tesis ini mengkaji kesan galaktosa atau fruktosa terhadap asimilasi sumber karbon sekunder seperti laktat oleh C. albicans. Gen C. albicans Isositrat liase (CaICL1) ditindas oleh penambahan 2% galaktosa atau fruktosa kepada sel-sel yang sedang membiak pada laktat. Gen CaICL1 yang mengekod kitaran glioksilat enzim Icl1 diperlukan untuk pertumbuhan dalam mengunakan sumber karbon sekunder seperti laktat. Enzim CaIcl1 didapati stabil dalam galaktosa tetapi tidak stabil dalam fruktosa. Sebaliknya, kedua-dua galaktosa dan fruktosa mendegradasikan protein S. cerevisiae Icl1 (ScIcl1). The virulence of C. albicans is dependent upon fitness attributes as well as virulence factors. These attributes include robust stress responses and metabolic flexibility. C. albicans can occupy a variety niches in human, many of which contain a range of different carbon sources. The assimilation of these carbon sources is important for growth and essential for establishment of infections by C. albicans. This thesis examines the impact of galactose or fructose upon the assimilation of secondary carbon sources such as lactate by C. albicans Isocitrate lyase gene (CaICL1) is repressed upon addition of 2% galactose or fructose to lactate- grown cells. The CaICL1 gene, which encode the glyoxylate cycles enzymes isocitrate lyase are required for growth on non- fermentable carbon sources such as lactate. However the enzyme CaIcl1 was not destabilized by galactose, but was degraded in response to fructose. In contrast, S. cerevisiae Icl1 (ScIcl1) was rapidly degraded in response to either galactose or fructose. 2016-02 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/31499/1/TING_SENG_YEAT_24.pdf Ting , Seng Yeat (2016) Effects Of Galactose And Fructose On The Expression Of Isocitrate Lyase (Icl1) Enzyme In Candida Albicans. Masters thesis, Universiti Sains Malaysia.
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic RK1-715 Dentistry
spellingShingle RK1-715 Dentistry
Ting , Seng Yeat
Effects Of Galactose And Fructose On The Expression Of Isocitrate Lyase (Icl1) Enzyme In Candida Albicans
description Sifat kepatogenan dalam C. albicans bergantung kepada atribut kecergasan dan juga faktor-faktor virulen yang lain. Ini termasuk sifat kental terhadap tindak balas tekanan dan penyesuaian metabolik. C. albicans boleh menduduki kebanyakkan persekitaran dalam ruang badan manusia yang mengandungi kepelbagaian sumber karbon yang berbeza. Asimilasi sumber karbon adalah penting untuk pertumbuhan dan juga untuk meneruskan jangkitan. Tesis ini mengkaji kesan galaktosa atau fruktosa terhadap asimilasi sumber karbon sekunder seperti laktat oleh C. albicans. Gen C. albicans Isositrat liase (CaICL1) ditindas oleh penambahan 2% galaktosa atau fruktosa kepada sel-sel yang sedang membiak pada laktat. Gen CaICL1 yang mengekod kitaran glioksilat enzim Icl1 diperlukan untuk pertumbuhan dalam mengunakan sumber karbon sekunder seperti laktat. Enzim CaIcl1 didapati stabil dalam galaktosa tetapi tidak stabil dalam fruktosa. Sebaliknya, kedua-dua galaktosa dan fruktosa mendegradasikan protein S. cerevisiae Icl1 (ScIcl1). The virulence of C. albicans is dependent upon fitness attributes as well as virulence factors. These attributes include robust stress responses and metabolic flexibility. C. albicans can occupy a variety niches in human, many of which contain a range of different carbon sources. The assimilation of these carbon sources is important for growth and essential for establishment of infections by C. albicans. This thesis examines the impact of galactose or fructose upon the assimilation of secondary carbon sources such as lactate by C. albicans Isocitrate lyase gene (CaICL1) is repressed upon addition of 2% galactose or fructose to lactate- grown cells. The CaICL1 gene, which encode the glyoxylate cycles enzymes isocitrate lyase are required for growth on non- fermentable carbon sources such as lactate. However the enzyme CaIcl1 was not destabilized by galactose, but was degraded in response to fructose. In contrast, S. cerevisiae Icl1 (ScIcl1) was rapidly degraded in response to either galactose or fructose.
format Thesis
author Ting , Seng Yeat
author_facet Ting , Seng Yeat
author_sort Ting , Seng Yeat
title Effects Of Galactose And Fructose On The Expression Of Isocitrate Lyase (Icl1) Enzyme In Candida Albicans
title_short Effects Of Galactose And Fructose On The Expression Of Isocitrate Lyase (Icl1) Enzyme In Candida Albicans
title_full Effects Of Galactose And Fructose On The Expression Of Isocitrate Lyase (Icl1) Enzyme In Candida Albicans
title_fullStr Effects Of Galactose And Fructose On The Expression Of Isocitrate Lyase (Icl1) Enzyme In Candida Albicans
title_full_unstemmed Effects Of Galactose And Fructose On The Expression Of Isocitrate Lyase (Icl1) Enzyme In Candida Albicans
title_sort effects of galactose and fructose on the expression of isocitrate lyase (icl1) enzyme in candida albicans
publishDate 2016
url http://eprints.usm.my/31499/1/TING_SENG_YEAT_24.pdf
http://eprints.usm.my/31499/
_version_ 1643707410736480256
score 13.211869

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