Bloactlve Protein Produced By Lactobacillus Plantarum Atcc 8014 In The Presence Of Allium Sativum

Several studies have reported that sub inhibitory concentrations of antibiotics or antimicrobials are capable to modulate bacteria transcription processes. Therefore, the bacteria might have introduced new proteins in mild stress surroundings like in the presence of antimicrobial agents at low co...

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Main Authors: lsmail, lsmatul Nurul Asyikin, Mohd Noor, Hanina, Muhamad, Hairul Shahril, Mat Radzil, Salina, Abdul Kader, Abdul Jalil, Mohamed Rehan, Maryam, Mohamad, Rosfarizan
Format: Conference Paper
Language:en_US
Published: Mines Wellness Hotel, Malaysia 2015
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Online Access:http://ddms.usim.edu.my/handle/123456789/9422
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spelling my.usim-94222015-09-17T04:19:08Z Bloactlve Protein Produced By Lactobacillus Plantarum Atcc 8014 In The Presence Of Allium Sativum lsmail, lsmatul Nurul Asyikin Mohd Noor, Hanina Muhamad, Hairul Shahril Mat Radzil, Salina Abdul Kader, Abdul Jalil Mohamed Rehan, Maryam Mohamad, Rosfarizan antimicrobial transcription sub-MIC proteins Lactobacillus plantarum ATCC 80 1 4 Allium sativum Several studies have reported that sub inhibitory concentrations of antibiotics or antimicrobials are capable to modulate bacteria transcription processes. Therefore, the bacteria might have introduced new proteins in mild stress surroundings like in the presence of antimicrobial agents at low concentrations. However, there are limited elucidations about unexpected ability of natural antimicrobial compounds to become a signaling agent capable to induce biological functions in bacteria at low concentrations. Thus, this present study aims to e'xplore the proteins production by Lactobacillus plantarum ATCC 8014 in the presence of Allium sativum at sub-minimal inhibitory concentration (sub-MIC). The Minimum Inhibition Concentration (MIC) of A. sativum against L. plantarum was 33.33% from microdilution assay. L. plantarum cells were treated with A. sativum at sub-MIC (0.05 x MIC) in the fermentation process. Three new protein bands (approximate size of 97.83 kD, 53.56 kD and 46.71 kD) were detected by SDS-PAGE profile for the treated bacteria. LC-MSIMS analysis identified 11 possible proteins from the three protein bands expressed in mild stress condition. The proteins showed antimicrobial activity toward several Gram-positive and I Gram-negative bacteria. Hence, L. plantarum ATCC 8014 in mild stress condition with the presence of 0.05 x MIC A. sativum could regulate bioactive proteins production. 2015-09-17T04:19:08Z 2015-09-17T04:19:08Z 2014-01-21 Conference Paper http://ddms.usim.edu.my/handle/123456789/9422 en_US Mines Wellness Hotel, Malaysia
institution Universiti Sains Islam Malaysia
building USIM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universit Sains Islam i Malaysia
content_source USIM Institutional Repository
url_provider http://ddms.usim.edu.my/
language en_US
topic antimicrobial
transcription
sub-MIC
proteins
Lactobacillus plantarum ATCC 80 1 4
Allium sativum
spellingShingle antimicrobial
transcription
sub-MIC
proteins
Lactobacillus plantarum ATCC 80 1 4
Allium sativum
lsmail, lsmatul Nurul Asyikin
Mohd Noor, Hanina
Muhamad, Hairul Shahril
Mat Radzil, Salina
Abdul Kader, Abdul Jalil
Mohamed Rehan, Maryam
Mohamad, Rosfarizan
Bloactlve Protein Produced By Lactobacillus Plantarum Atcc 8014 In The Presence Of Allium Sativum
description Several studies have reported that sub inhibitory concentrations of antibiotics or antimicrobials are capable to modulate bacteria transcription processes. Therefore, the bacteria might have introduced new proteins in mild stress surroundings like in the presence of antimicrobial agents at low concentrations. However, there are limited elucidations about unexpected ability of natural antimicrobial compounds to become a signaling agent capable to induce biological functions in bacteria at low concentrations. Thus, this present study aims to e'xplore the proteins production by Lactobacillus plantarum ATCC 8014 in the presence of Allium sativum at sub-minimal inhibitory concentration (sub-MIC). The Minimum Inhibition Concentration (MIC) of A. sativum against L. plantarum was 33.33% from microdilution assay. L. plantarum cells were treated with A. sativum at sub-MIC (0.05 x MIC) in the fermentation process. Three new protein bands (approximate size of 97.83 kD, 53.56 kD and 46.71 kD) were detected by SDS-PAGE profile for the treated bacteria. LC-MSIMS analysis identified 11 possible proteins from the three protein bands expressed in mild stress condition. The proteins showed antimicrobial activity toward several Gram-positive and I Gram-negative bacteria. Hence, L. plantarum ATCC 8014 in mild stress condition with the presence of 0.05 x MIC A. sativum could regulate bioactive proteins production.
format Conference Paper
author lsmail, lsmatul Nurul Asyikin
Mohd Noor, Hanina
Muhamad, Hairul Shahril
Mat Radzil, Salina
Abdul Kader, Abdul Jalil
Mohamed Rehan, Maryam
Mohamad, Rosfarizan
author_facet lsmail, lsmatul Nurul Asyikin
Mohd Noor, Hanina
Muhamad, Hairul Shahril
Mat Radzil, Salina
Abdul Kader, Abdul Jalil
Mohamed Rehan, Maryam
Mohamad, Rosfarizan
author_sort lsmail, lsmatul Nurul Asyikin
title Bloactlve Protein Produced By Lactobacillus Plantarum Atcc 8014 In The Presence Of Allium Sativum
title_short Bloactlve Protein Produced By Lactobacillus Plantarum Atcc 8014 In The Presence Of Allium Sativum
title_full Bloactlve Protein Produced By Lactobacillus Plantarum Atcc 8014 In The Presence Of Allium Sativum
title_fullStr Bloactlve Protein Produced By Lactobacillus Plantarum Atcc 8014 In The Presence Of Allium Sativum
title_full_unstemmed Bloactlve Protein Produced By Lactobacillus Plantarum Atcc 8014 In The Presence Of Allium Sativum
title_sort bloactlve protein produced by lactobacillus plantarum atcc 8014 in the presence of allium sativum
publisher Mines Wellness Hotel, Malaysia
publishDate 2015
url http://ddms.usim.edu.my/handle/123456789/9422
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score 13.222552