Staff View: Protein Produced by Bacillus subtilis ATCC21332 in the Presence of Cymbopogon flexuosus Essential oil

Protein Produced by Bacillus subtilis ATCC21332 in the Presence of Cymbopogon flexuosus Essential oil

Proteins levels produced by bacteria may be increased in stressful surroundings, such as in the presence of antibiotics. It appears that many antimicrobial agents or antibiotics, when used at low concentrations, have in common the ability to activate or repress gene transcription, which is distinct...

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Main Authors:	Hanina Mohd, Noor,, Hairul Shahril, Muhamad,, Ismatul Nurul Asyikin, Ismail,, Salina Mat, Radzi,, Maryam Mohamed, Rehan,, Abdul Jalil, Abdul Kader,
Format:	Conference Paper
Language:	en_US
Published:	Trans Tech Publications Ltd 2015
Subjects:	Bacillus subtilis ATCC21332 Cymbopogon flexuosus essential oil Protein
Online Access:	http://ddms.usim.edu.my/handle/123456789/8974
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id	my.usim-8974
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spelling	my.usim-89742015-08-05T04:19:07Z Protein Produced by Bacillus subtilis ATCC21332 in the Presence of Cymbopogon flexuosus Essential oil Hanina Mohd, Noor, Hairul Shahril, Muhamad, Ismatul Nurul Asyikin, Ismail, Salina Mat, Radzi, Maryam Mohamed, Rehan, Abdul Jalil, Abdul Kader, Bacillus subtilis ATCC21332 Cymbopogon flexuosus essential oil Protein Proteins levels produced by bacteria may be increased in stressful surroundings, such as in the presence of antibiotics. It appears that many antimicrobial agents or antibiotics, when used at low concentrations, have in common the ability to activate or repress gene transcription, which is distinct from their inhibitory effect. There have been comparatively few studies on the potential of antibiotics or natural compounds in nature as a specific chemical signal that can trigger a variety of biological functions. Therefore, this study was focusing on the effect of essential oil from Cymbopogon flexuosus in regulating proteins production by Bacillus subtilis ATCC21332. The Minimum Inhibition Concentration (MIC) of the C. flexuosus essential oil on B. subtilis was determined by using microdilution assay, resulting 1.76mg/ml. The bacteria cells were further exposed to the C. flexuosus essential oil at concentration of 0.01 MIC for 72 h. The proteins were then isolated and analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Proteins profile showed that a band with approximate size of 30 kDa was appeared for the treated bacteria with C. flexuosus essential oil. Thus, B. subtilis ATCC21332 in stressful condition with the presence of C. flexuosus essential oils at low concentration could induce the protein production. The isolated protein also showed antimicrobial activity against selected Gram-positive and Gram-negative bacteria. 2015-08-05T04:19:07Z 2015-08-05T04:19:07Z 2014-01-01 Conference Paper 1013-9826 http://ddms.usim.edu.my/handle/123456789/8974 en_US Trans Tech Publications Ltd
institution	Universiti Sains Islam Malaysia
building	USIM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universit Sains Islam i Malaysia
content_source	USIM Institutional Repository
url_provider	http://ddms.usim.edu.my/
language	en_US
topic	Bacillus subtilis ATCC21332 Cymbopogon flexuosus essential oil Protein
spellingShingle	Bacillus subtilis ATCC21332 Cymbopogon flexuosus essential oil Protein Hanina Mohd, Noor, Hairul Shahril, Muhamad, Ismatul Nurul Asyikin, Ismail, Salina Mat, Radzi, Maryam Mohamed, Rehan, Abdul Jalil, Abdul Kader, Protein Produced by Bacillus subtilis ATCC21332 in the Presence of Cymbopogon flexuosus Essential oil
description	Proteins levels produced by bacteria may be increased in stressful surroundings, such as in the presence of antibiotics. It appears that many antimicrobial agents or antibiotics, when used at low concentrations, have in common the ability to activate or repress gene transcription, which is distinct from their inhibitory effect. There have been comparatively few studies on the potential of antibiotics or natural compounds in nature as a specific chemical signal that can trigger a variety of biological functions. Therefore, this study was focusing on the effect of essential oil from Cymbopogon flexuosus in regulating proteins production by Bacillus subtilis ATCC21332. The Minimum Inhibition Concentration (MIC) of the C. flexuosus essential oil on B. subtilis was determined by using microdilution assay, resulting 1.76mg/ml. The bacteria cells were further exposed to the C. flexuosus essential oil at concentration of 0.01 MIC for 72 h. The proteins were then isolated and analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Proteins profile showed that a band with approximate size of 30 kDa was appeared for the treated bacteria with C. flexuosus essential oil. Thus, B. subtilis ATCC21332 in stressful condition with the presence of C. flexuosus essential oils at low concentration could induce the protein production. The isolated protein also showed antimicrobial activity against selected Gram-positive and Gram-negative bacteria.
format	Conference Paper
author	Hanina Mohd, Noor, Hairul Shahril, Muhamad, Ismatul Nurul Asyikin, Ismail, Salina Mat, Radzi, Maryam Mohamed, Rehan, Abdul Jalil, Abdul Kader,
author_facet	Hanina Mohd, Noor, Hairul Shahril, Muhamad, Ismatul Nurul Asyikin, Ismail, Salina Mat, Radzi, Maryam Mohamed, Rehan, Abdul Jalil, Abdul Kader,
author_sort	Hanina Mohd, Noor,
title	Protein Produced by Bacillus subtilis ATCC21332 in the Presence of Cymbopogon flexuosus Essential oil
title_short	Protein Produced by Bacillus subtilis ATCC21332 in the Presence of Cymbopogon flexuosus Essential oil
title_full	Protein Produced by Bacillus subtilis ATCC21332 in the Presence of Cymbopogon flexuosus Essential oil
title_fullStr	Protein Produced by Bacillus subtilis ATCC21332 in the Presence of Cymbopogon flexuosus Essential oil
title_full_unstemmed	Protein Produced by Bacillus subtilis ATCC21332 in the Presence of Cymbopogon flexuosus Essential oil
title_sort	protein produced by bacillus subtilis atcc21332 in the presence of cymbopogon flexuosus essential oil
publisher	Trans Tech Publications Ltd
publishDate	2015
url	http://ddms.usim.edu.my/handle/123456789/8974
_version_	1645152511467716608
score	13.222552

Protein Produced by Bacillus subtilis ATCC21332 in the Presence of Cymbopogon flexuosus Essential oil

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