Staff View: Effect of heat and filter sterilization on the efficiency of coleus aromaticus as an antibacterial agent against diabetic wound pathogens

Effect of heat and filter sterilization on the efficiency of coleus aromaticus as an antibacterial agent against diabetic wound pathogens

Objective: The purpose of the present study is to evaluate the effect of sterilization on the efficiency of Coleus aromaticus on eradicating diabetic wound pathogens.Methods: The investigation was explored by deploying disc diffusion, Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Conc...

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Main Authors:	K., Muniandy,, Z., Hassan,, M.H., Mohd Isa,
Format:	Article
Language:	en_US
Published:	International Journal of Pharmacy and Pharmaceutical Science 2015
Subjects:	Coleus aromaticus Disc diffusion Filter sterilization Heat sterilization Minimum bactericidal concentration Minimumm inhibitory concentration Time kill assay Wound pathogens
Online Access:	http://ddms.usim.edu.my/handle/123456789/8787 http://innovareacademics.in/journals/index.php/ijpps/article/view/2636/pdf_327
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spelling	my.usim-87872015-12-31T07:47:32Z Effect of heat and filter sterilization on the efficiency of coleus aromaticus as an antibacterial agent against diabetic wound pathogens K., Muniandy, Z., Hassan, M.H., Mohd Isa, Coleus aromaticus Disc diffusion Filter sterilization Heat sterilization Minimum bactericidal concentration Minimumm inhibitory concentration Time kill assay Wound pathogens Objective: The purpose of the present study is to evaluate the effect of sterilization on the efficiency of Coleus aromaticus on eradicating diabetic wound pathogens.Methods: The investigation was explored by deploying disc diffusion, Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and time kill studies on Escherichia coli, Staphylococcus aureus, Klebsiella pneumonia, Pseudomonas aeruginosa and Proteus mirabilis.Results: Zone of inhibition was 17.5-27.0 mm for unsterilized extract, 11.0-20.0 mm for heat sterilized extract and 6.0-12.0 mm for filter sterilized extract. MIC ranged from 1.02-2.60 mg/ml for unsterilized extract, 2.60-5.21 mg/ml for heat sterilized extract and 8.33-18.75 mg/ml for filter sterilized extract. MBC ranged from 2.60-8.33 mg/ml for unsterilized extract and much higher values were noted with heat and filter sterilized extract. Time kill assay showed log reduction of more than 3 in 2 x MIC unsterilized extract on microorganisms tested. Both heat and filter sterilized extract didn’t show 3 log or more reduction even at 2 x MIC. However, better log reduction was observed in heat sterilized extract compared to filter sterilized extract.Conclusion: C. aromaticus is a potent antibacterial agent and heat sterilization could be used to prepare this agent pharmaceutically. The evaluation on the effect of other sterilization methods on its efficiency as an antimicrobial wound remedy is highly suggested. 2015-07-13T07:46:41Z 2015-07-13T07:46:41Z 2014 Article 9751491 http://ddms.usim.edu.my/handle/123456789/8787 http://innovareacademics.in/journals/index.php/ijpps/article/view/2636/pdf_327 en_US International Journal of Pharmacy and Pharmaceutical Science
institution	Universiti Sains Islam Malaysia
building	USIM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universit Sains Islam i Malaysia
content_source	USIM Institutional Repository
url_provider	http://ddms.usim.edu.my/
language	en_US
topic	Coleus aromaticus Disc diffusion Filter sterilization Heat sterilization Minimum bactericidal concentration Minimumm inhibitory concentration Time kill assay Wound pathogens
spellingShingle	Coleus aromaticus Disc diffusion Filter sterilization Heat sterilization Minimum bactericidal concentration Minimumm inhibitory concentration Time kill assay Wound pathogens K., Muniandy, Z., Hassan, M.H., Mohd Isa, Effect of heat and filter sterilization on the efficiency of coleus aromaticus as an antibacterial agent against diabetic wound pathogens
description	Objective: The purpose of the present study is to evaluate the effect of sterilization on the efficiency of Coleus aromaticus on eradicating diabetic wound pathogens.Methods: The investigation was explored by deploying disc diffusion, Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and time kill studies on Escherichia coli, Staphylococcus aureus, Klebsiella pneumonia, Pseudomonas aeruginosa and Proteus mirabilis.Results: Zone of inhibition was 17.5-27.0 mm for unsterilized extract, 11.0-20.0 mm for heat sterilized extract and 6.0-12.0 mm for filter sterilized extract. MIC ranged from 1.02-2.60 mg/ml for unsterilized extract, 2.60-5.21 mg/ml for heat sterilized extract and 8.33-18.75 mg/ml for filter sterilized extract. MBC ranged from 2.60-8.33 mg/ml for unsterilized extract and much higher values were noted with heat and filter sterilized extract. Time kill assay showed log reduction of more than 3 in 2 x MIC unsterilized extract on microorganisms tested. Both heat and filter sterilized extract didn’t show 3 log or more reduction even at 2 x MIC. However, better log reduction was observed in heat sterilized extract compared to filter sterilized extract.Conclusion: C. aromaticus is a potent antibacterial agent and heat sterilization could be used to prepare this agent pharmaceutically. The evaluation on the effect of other sterilization methods on its efficiency as an antimicrobial wound remedy is highly suggested.
format	Article
author	K., Muniandy, Z., Hassan, M.H., Mohd Isa,
author_facet	K., Muniandy, Z., Hassan, M.H., Mohd Isa,
author_sort	K., Muniandy,
title	Effect of heat and filter sterilization on the efficiency of coleus aromaticus as an antibacterial agent against diabetic wound pathogens
title_short	Effect of heat and filter sterilization on the efficiency of coleus aromaticus as an antibacterial agent against diabetic wound pathogens
title_full	Effect of heat and filter sterilization on the efficiency of coleus aromaticus as an antibacterial agent against diabetic wound pathogens
title_fullStr	Effect of heat and filter sterilization on the efficiency of coleus aromaticus as an antibacterial agent against diabetic wound pathogens
title_full_unstemmed	Effect of heat and filter sterilization on the efficiency of coleus aromaticus as an antibacterial agent against diabetic wound pathogens
title_sort	effect of heat and filter sterilization on the efficiency of coleus aromaticus as an antibacterial agent against diabetic wound pathogens
publisher	International Journal of Pharmacy and Pharmaceutical Science
publishDate	2015
url	http://ddms.usim.edu.my/handle/123456789/8787 http://innovareacademics.in/journals/index.php/ijpps/article/view/2636/pdf_327
_version_	1645152473871024128
score	13.222552

Effect of heat and filter sterilization on the efficiency of coleus aromaticus as an antibacterial agent against diabetic wound pathogens

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