Staff View: Detection of blood thrombin enzyme in surimi-based products by using polymerase chain reaction (PCR) method

Detection of blood thrombin enzyme in surimi-based products by using polymerase chain reaction (PCR) method

The detection of blood thrombin enzyme presented in eight samples of surimi based products was investigated by using Polymerase Chain Reaction (PCR) method. Specific primers for chicken (Gallus gallus), cow (Bos taurus) and pig (Sus sucrofa) blood thrombin enzyme were designed for positive detection...

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Main Authors:	A.R., Alina, A.S., Nur Nadiah Syuhada, N.R.S.A., Sharifah, A., Siti Mashitoh, A.S., Nurul Aqilah, A.H., Nurul Mawaddah, M.S., Nurulhuda, H.S., Ummi Syuhada, K.M.W., Syamsul, N., Nurul Farah Sakinah A. Basir,
Format:	Article
Language:	en_US
Published:	2015
Subjects:	Blood thrombin enzyme Polymerase chain reaction Surimi-based product
Online Access:	http://ddms.usim.edu.my/handle/123456789/8768
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id	my.usim-8768
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spelling	my.usim-87682017-02-23T07:14:28Z Detection of blood thrombin enzyme in surimi-based products by using polymerase chain reaction (PCR) method A.R., Alina A.S., Nur Nadiah Syuhada N.R.S.A., Sharifah A., Siti Mashitoh A.S., Nurul Aqilah A.H., Nurul Mawaddah M.S., Nurulhuda H.S., Ummi Syuhada K.M.W., Syamsul N., Nurul Farah Sakinah A. Basir, Blood thrombin enzyme Polymerase chain reaction Surimi-based product The detection of blood thrombin enzyme presented in eight samples of surimi based products was investigated by using Polymerase Chain Reaction (PCR) method. Specific primers for chicken (Gallus gallus), cow (Bos taurus) and pig (Sus sucrofa) blood thrombin enzyme were designed for positive detection. Two primers for chicken blood which are Gal2 and Gal3 showed 98% and 99% in significant identity of G.gallus coagulation factor II (thrombin) while two primers from cow which are Bos4 and Bos6 showed 100% significant identity of B. Taurus coagulation factor II (thrombin). On the contrary, there were no positive results on pig primers with S. sucrofa coagulation factor II (thrombin). PCR amplification with Gal2 and Bos4 primers in surimi based products showed several positive results while Sus5 primer showed none. Further research should be done to verify the consistency of this result and redesign a specific primer for pig's blood thrombin enzymes is vital in order to guarantee the quality of food products to comply with Halal food guidelines and regulations. © IDOSI Publications, 2012. 2015-07-13T03:19:52Z 2015-07-13T03:19:52Z 2012 Article 18184952 http://ddms.usim.edu.my/handle/123456789/8768 en_US
institution	Universiti Sains Islam Malaysia
building	USIM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universit Sains Islam i Malaysia
content_source	USIM Institutional Repository
url_provider	http://ddms.usim.edu.my/
language	en_US
topic	Blood thrombin enzyme Polymerase chain reaction Surimi-based product
spellingShingle	Blood thrombin enzyme Polymerase chain reaction Surimi-based product A.R., Alina A.S., Nur Nadiah Syuhada N.R.S.A., Sharifah A., Siti Mashitoh A.S., Nurul Aqilah A.H., Nurul Mawaddah M.S., Nurulhuda H.S., Ummi Syuhada K.M.W., Syamsul N., Nurul Farah Sakinah A. Basir, Detection of blood thrombin enzyme in surimi-based products by using polymerase chain reaction (PCR) method
description	The detection of blood thrombin enzyme presented in eight samples of surimi based products was investigated by using Polymerase Chain Reaction (PCR) method. Specific primers for chicken (Gallus gallus), cow (Bos taurus) and pig (Sus sucrofa) blood thrombin enzyme were designed for positive detection. Two primers for chicken blood which are Gal2 and Gal3 showed 98% and 99% in significant identity of G.gallus coagulation factor II (thrombin) while two primers from cow which are Bos4 and Bos6 showed 100% significant identity of B. Taurus coagulation factor II (thrombin). On the contrary, there were no positive results on pig primers with S. sucrofa coagulation factor II (thrombin). PCR amplification with Gal2 and Bos4 primers in surimi based products showed several positive results while Sus5 primer showed none. Further research should be done to verify the consistency of this result and redesign a specific primer for pig's blood thrombin enzymes is vital in order to guarantee the quality of food products to comply with Halal food guidelines and regulations. © IDOSI Publications, 2012.
format	Article
author	A.R., Alina A.S., Nur Nadiah Syuhada N.R.S.A., Sharifah A., Siti Mashitoh A.S., Nurul Aqilah A.H., Nurul Mawaddah M.S., Nurulhuda H.S., Ummi Syuhada K.M.W., Syamsul N., Nurul Farah Sakinah A. Basir,
author_facet	A.R., Alina A.S., Nur Nadiah Syuhada N.R.S.A., Sharifah A., Siti Mashitoh A.S., Nurul Aqilah A.H., Nurul Mawaddah M.S., Nurulhuda H.S., Ummi Syuhada K.M.W., Syamsul N., Nurul Farah Sakinah A. Basir,
author_sort	A.R., Alina
title	Detection of blood thrombin enzyme in surimi-based products by using polymerase chain reaction (PCR) method
title_short	Detection of blood thrombin enzyme in surimi-based products by using polymerase chain reaction (PCR) method
title_full	Detection of blood thrombin enzyme in surimi-based products by using polymerase chain reaction (PCR) method
title_fullStr	Detection of blood thrombin enzyme in surimi-based products by using polymerase chain reaction (PCR) method
title_full_unstemmed	Detection of blood thrombin enzyme in surimi-based products by using polymerase chain reaction (PCR) method
title_sort	detection of blood thrombin enzyme in surimi-based products by using polymerase chain reaction (pcr) method
publishDate	2015
url	http://ddms.usim.edu.my/handle/123456789/8768
_version_	1645152468688961536
score	13.222552

Detection of blood thrombin enzyme in surimi-based products by using polymerase chain reaction (PCR) method

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