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Cerapan mikroskopi dan analisis sitometri Streptococcus mutans yang ditindakkan dengan ekstrak batang Melastoma malabathricum L

Streptococcus mutans is the earliest bacteria that forms dental caries in humans. This study determines the effect of acetone extract from Melastoma malabathricum stem against S. mutans by microscopic observation and absorption of fluorescence dye. The extraction of M. malabathricum stem using aceto...

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Main Authors: M.H., Rohazila,, I., Nazlina,, W.A., Yaacob,
Format: Article
Language:en_US
Published: Malaysian Society of Applied Biology 2015
Subjects:
Cytometry analysis
Microscopic observation
Streptococcus mutans and melastoma malabathricum
Online Access:http://ddms.usim.edu.my/handle/123456789/8724
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spelling my.usim-87242017-02-23T03:08:53Z Cerapan mikroskopi dan analisis sitometri Streptococcus mutans yang ditindakkan dengan ekstrak batang Melastoma malabathricum L M.H., Rohazila, I., Nazlina, W.A., Yaacob, Cytometry analysis Microscopic observation Streptococcus mutans and melastoma malabathricum Streptococcus mutans is the earliest bacteria that forms dental caries in humans. This study determines the effect of acetone extract from Melastoma malabathricum stem against S. mutans by microscopic observation and absorption of fluorescence dye. The extraction of M. malabathricum stem using acetone yielded 0.5 g of acetone extract. Anti-bacterial activity determined by disk diffusion assay showed that stem extract at the 30 mg/mL inhibit bacterial growth with inhibition zone diameter of 15 mm. The effect of the extract towards S. mutans by microscopic observation was done by Scanning Electron Microscope (SEM) and Transmission Electronic Microscope (TEM). Result from microscopic observation showed that the M. malabathricum extract damaged the cell’s shape by rupturing the surface and the wall, causing the cytoplasm to leak. The assay using propidium iodide (PI) as the fluorescence dye showed that it was absorbed into the observed cell through flow cytometry analysis. The absorption of propidium iodide in treated cell confirmed the possibility of disturbance in the permeability of the cell membrane’s structure. From the microscopic observation and cytometry analysis, it can be concluded that acetone extract of M. malabathricum act as anti-bacteria agent that cause damage to the cell wall and disturb the permeability of the membrane structure. 2015-07-08T03:07:08Z 2015-07-08T03:07:08Z 2014 Article 1268643 http://ddms.usim.edu.my/handle/123456789/8724 en_US Malaysian Society of Applied Biology
institution Universiti Sains Islam Malaysia
building USIM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universit Sains Islam i Malaysia
content_source USIM Institutional Repository
url_provider http://ddms.usim.edu.my/
language en_US
topic Cytometry analysis
Microscopic observation
Streptococcus mutans and melastoma malabathricum
spellingShingle Cytometry analysis
Microscopic observation
Streptococcus mutans and melastoma malabathricum
M.H., Rohazila,
I., Nazlina,
W.A., Yaacob,
Cerapan mikroskopi dan analisis sitometri Streptococcus mutans yang ditindakkan dengan ekstrak batang Melastoma malabathricum L
description Streptococcus mutans is the earliest bacteria that forms dental caries in humans. This study determines the effect of acetone extract from Melastoma malabathricum stem against S. mutans by microscopic observation and absorption of fluorescence dye. The extraction of M. malabathricum stem using acetone yielded 0.5 g of acetone extract. Anti-bacterial activity determined by disk diffusion assay showed that stem extract at the 30 mg/mL inhibit bacterial growth with inhibition zone diameter of 15 mm. The effect of the extract towards S. mutans by microscopic observation was done by Scanning Electron Microscope (SEM) and Transmission Electronic Microscope (TEM). Result from microscopic observation showed that the M. malabathricum extract damaged the cell’s shape by rupturing the surface and the wall, causing the cytoplasm to leak. The assay using propidium iodide (PI) as the fluorescence dye showed that it was absorbed into the observed cell through flow cytometry analysis. The absorption of propidium iodide in treated cell confirmed the possibility of disturbance in the permeability of the cell membrane’s structure. From the microscopic observation and cytometry analysis, it can be concluded that acetone extract of M. malabathricum act as anti-bacteria agent that cause damage to the cell wall and disturb the permeability of the membrane structure.
format Article
author M.H., Rohazila,
I., Nazlina,
W.A., Yaacob,
author_facet M.H., Rohazila,
I., Nazlina,
W.A., Yaacob,
author_sort M.H., Rohazila,
title Cerapan mikroskopi dan analisis sitometri Streptococcus mutans yang ditindakkan dengan ekstrak batang Melastoma malabathricum L
title_short Cerapan mikroskopi dan analisis sitometri Streptococcus mutans yang ditindakkan dengan ekstrak batang Melastoma malabathricum L
title_full Cerapan mikroskopi dan analisis sitometri Streptococcus mutans yang ditindakkan dengan ekstrak batang Melastoma malabathricum L
title_fullStr Cerapan mikroskopi dan analisis sitometri Streptococcus mutans yang ditindakkan dengan ekstrak batang Melastoma malabathricum L
title_full_unstemmed Cerapan mikroskopi dan analisis sitometri Streptococcus mutans yang ditindakkan dengan ekstrak batang Melastoma malabathricum L
title_sort cerapan mikroskopi dan analisis sitometri streptococcus mutans yang ditindakkan dengan ekstrak batang melastoma malabathricum l
publisher Malaysian Society of Applied Biology
publishDate 2015
url http://ddms.usim.edu.my/handle/123456789/8724
_version_ 1645152457818374144
score 13.211869

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