Cover Image

Molybdate reduction to molybdenum blue by an antarctic bacterium

A molybdenum-reducing bacterium from Antarctica has been isolated. The bacterium converts sodium molybdate or Mo6+ to molybdenum blue (Mo-blue). Electron donors such as glucose, sucrose, fructose, and lactose supported molybdate reduction. Ammonium sulphate was the best nitrogen source for molybdate...

Full description

Saved in:
Bibliographic Details
Main Authors: S.A., Ahmad,, M.Y., Shukor,, N.A., Shamaan,, W.P., Mac Cormack,, M.A., Syed,
Format: Article
Language:en_US
Published: Asian Research Publishing Network 2015
Subjects:
Hydrogen-Ion Concentration
molybdenum blue
molybdic acid
metabolism
Online Access:http://ddms.usim.edu.my/handle/123456789/8710
http://www.hindawi.com/journals/bmri/2013/871941/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.usim-8710
record_format dspace
spelling my.usim-87102015-12-29T03:02:20Z Molybdate reduction to molybdenum blue by an antarctic bacterium S.A., Ahmad, M.Y., Shukor, N.A., Shamaan, W.P., Mac Cormack, M.A., Syed, Hydrogen-Ion Concentration molybdenum blue molybdic acid metabolism A molybdenum-reducing bacterium from Antarctica has been isolated. The bacterium converts sodium molybdate or Mo6+ to molybdenum blue (Mo-blue). Electron donors such as glucose, sucrose, fructose, and lactose supported molybdate reduction. Ammonium sulphate was the best nitrogen source for molybdate reduction. Optimal conditions for molybdate reduction were between 30 and 50 mM molybdate, between 15 and 20°C, and initial pH between 6.5 and 7.5. The Mo-blue produced had a unique absorption spectrum with a peak maximum at 865 nm and a shoulder at 710 nm. Respiratory inhibitors such as antimycin A, sodium azide, potassium cyanide, and rotenone failed to inhibit the reducing activity. The Mo-reducing enzyme was partially purified using ion exchange and gel filtration chromatography. The partially purified enzyme showed optimal pH and temperature for activity at 6.0 and 20°C, respectively. Metal ions such as cadmium, chromium, copper, silver, lead, and mercury caused more than 95% inhibition of the molybdenum-reducing activity at 0.1 mM. The isolate was tentatively identified as Pseudomonas sp. strain DRY1 based on partial 16s rDNA molecular phylogenetic assessment and the Biolog microbial identification system. The characteristics of this strain would make it very useful in bioremediation works in the polar and temperate countries. © 2013 S. A. Ahmad et al. 2015-07-07T06:33:05Z 2015-07-07T06:33:05Z 2013 Article 23146133 http://ddms.usim.edu.my/handle/123456789/8710 http://www.hindawi.com/journals/bmri/2013/871941/ en_US Asian Research Publishing Network
institution Universiti Sains Islam Malaysia
building USIM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universit Sains Islam i Malaysia
content_source USIM Institutional Repository
url_provider http://ddms.usim.edu.my/
language en_US
topic Hydrogen-Ion Concentration
molybdenum blue
molybdic acid
metabolism
spellingShingle Hydrogen-Ion Concentration
molybdenum blue
molybdic acid
metabolism
S.A., Ahmad,
M.Y., Shukor,
N.A., Shamaan,
W.P., Mac Cormack,
M.A., Syed,
Molybdate reduction to molybdenum blue by an antarctic bacterium
description A molybdenum-reducing bacterium from Antarctica has been isolated. The bacterium converts sodium molybdate or Mo6+ to molybdenum blue (Mo-blue). Electron donors such as glucose, sucrose, fructose, and lactose supported molybdate reduction. Ammonium sulphate was the best nitrogen source for molybdate reduction. Optimal conditions for molybdate reduction were between 30 and 50 mM molybdate, between 15 and 20°C, and initial pH between 6.5 and 7.5. The Mo-blue produced had a unique absorption spectrum with a peak maximum at 865 nm and a shoulder at 710 nm. Respiratory inhibitors such as antimycin A, sodium azide, potassium cyanide, and rotenone failed to inhibit the reducing activity. The Mo-reducing enzyme was partially purified using ion exchange and gel filtration chromatography. The partially purified enzyme showed optimal pH and temperature for activity at 6.0 and 20°C, respectively. Metal ions such as cadmium, chromium, copper, silver, lead, and mercury caused more than 95% inhibition of the molybdenum-reducing activity at 0.1 mM. The isolate was tentatively identified as Pseudomonas sp. strain DRY1 based on partial 16s rDNA molecular phylogenetic assessment and the Biolog microbial identification system. The characteristics of this strain would make it very useful in bioremediation works in the polar and temperate countries. © 2013 S. A. Ahmad et al.
format Article
author S.A., Ahmad,
M.Y., Shukor,
N.A., Shamaan,
W.P., Mac Cormack,
M.A., Syed,
author_facet S.A., Ahmad,
M.Y., Shukor,
N.A., Shamaan,
W.P., Mac Cormack,
M.A., Syed,
author_sort S.A., Ahmad,
title Molybdate reduction to molybdenum blue by an antarctic bacterium
title_short Molybdate reduction to molybdenum blue by an antarctic bacterium
title_full Molybdate reduction to molybdenum blue by an antarctic bacterium
title_fullStr Molybdate reduction to molybdenum blue by an antarctic bacterium
title_full_unstemmed Molybdate reduction to molybdenum blue by an antarctic bacterium
title_sort molybdate reduction to molybdenum blue by an antarctic bacterium
publisher Asian Research Publishing Network
publishDate 2015
url http://ddms.usim.edu.my/handle/123456789/8710
http://www.hindawi.com/journals/bmri/2013/871941/
_version_ 1645152454633848832
score 13.222552

Similar Items