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Effects Of Cymbopogon Sp. Essential Oils And Streptomycin Sulfate On Antimicrobial Proteins Production By Bacillus Subtilis Atcc 21332

Proteins level produced by bacteria may be increased in stressful surroundings, such as in the presence of antimicrobial agent. It appears that many antimicrobial agents, when used at low concentrations, have in common the ability to activate or repress gene transcription, which is distinct from...

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Main Author: Hairul Shahril Bin Muhamad
Format: Newspaper Article
Language:English
Published: Universiti Sains Islam Malaysia 2015
Subjects:
Effects of Cymbopogon
Essential Oils and Streptomycin Sulfate
Antimicrobial Proteins Production
Bacillus subtilis ATCC 21332
Online Access:http://ddms.usim.edu.my/handle/123456789/8629
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spelling my.usim-86292017-03-02T07:01:14Z Effects Of Cymbopogon Sp. Essential Oils And Streptomycin Sulfate On Antimicrobial Proteins Production By Bacillus Subtilis Atcc 21332 Hairul Shahril Bin Muhamad Effects of Cymbopogon Essential Oils and Streptomycin Sulfate Antimicrobial Proteins Production Bacillus subtilis ATCC 21332 Proteins level produced by bacteria may be increased in stressful surroundings, such as in the presence of antimicrobial agent. It appears that many antimicrobial agents, when used at low concentrations, have in common the ability to activate or repress gene transcription, which is distinct from their inhibitory effect. However, there have been comparatively few studies on the potential of natural compounds as a specific chemical signal that can trigger a variety of biological functions. Therefore, the aim of this study is to evaluate the effects of Cymbopogon sp. (which are C. nardus and C. flexuosus) essential oils and Streptomycin Sulfate in regulating proteins or peptides production by Bacillus subtilis ATCC 21332. Results of Microdilution assay showed that the Minimum Inhibition Concentrations (MICs) of C. nardus and C. flexuosus as well as Streptomycin Sulfate on B. subtilis ATCC 21332 were 1.56% (v/v), 0.2% (v/v) and 2.5 mg/ml respectively. C. flexuosus and C. nardus essential oils at concentration of 0.01 MIC, each was added during early log phase of bacterial growth at 37ºC, resulting the production of new intracellular proteins with similar approximate size of 180 kDa in which recognized as DNA-directed RNA polymerase β subunit enzyme and respiratory nitrate reductase α subunit enzyme respectively. Besides, when B. subtilis ATCC 21332 were induced by C. flexuosus essential oil and Streptomycin Sulfate during log phase of growing cells at 30ºC could also secrete the extracellular proteins with approximate size of 30 kDa, identified as Bacillopeptidase F. In addition, B. subtilis ATCC 21332 in the presence of either C. nardus or C. flexuosus essential oils could secrete the bioactive extracellular proteins with potent antimicrobial activity against certain Gram-positive and Gram-negative bacteria. Hence, B. subtilis ATCC 21332 in stressful condition with the presence of either Cymbopogon sp. essential oils or Streptomycin Sulfate at 0.01 MIC level were able to induce the production or secretion of bioactive proteins. 2015-07-06T02:29:55Z 2015-07-06T02:29:55Z 2014-03 Newspaper Article http://ddms.usim.edu.my/handle/123456789/8629 en Universiti Sains Islam Malaysia
institution Universiti Sains Islam Malaysia
building USIM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universit Sains Islam i Malaysia
content_source USIM Institutional Repository
url_provider http://ddms.usim.edu.my/
language English
topic Effects of Cymbopogon
Essential Oils and Streptomycin Sulfate
Antimicrobial Proteins Production
Bacillus subtilis ATCC 21332
spellingShingle Effects of Cymbopogon
Essential Oils and Streptomycin Sulfate
Antimicrobial Proteins Production
Bacillus subtilis ATCC 21332
Hairul Shahril Bin Muhamad
Effects Of Cymbopogon Sp. Essential Oils And Streptomycin Sulfate On Antimicrobial Proteins Production By Bacillus Subtilis Atcc 21332
description Proteins level produced by bacteria may be increased in stressful surroundings, such as in the presence of antimicrobial agent. It appears that many antimicrobial agents, when used at low concentrations, have in common the ability to activate or repress gene transcription, which is distinct from their inhibitory effect. However, there have been comparatively few studies on the potential of natural compounds as a specific chemical signal that can trigger a variety of biological functions. Therefore, the aim of this study is to evaluate the effects of Cymbopogon sp. (which are C. nardus and C. flexuosus) essential oils and Streptomycin Sulfate in regulating proteins or peptides production by Bacillus subtilis ATCC 21332. Results of Microdilution assay showed that the Minimum Inhibition Concentrations (MICs) of C. nardus and C. flexuosus as well as Streptomycin Sulfate on B. subtilis ATCC 21332 were 1.56% (v/v), 0.2% (v/v) and 2.5 mg/ml respectively. C. flexuosus and C. nardus essential oils at concentration of 0.01 MIC, each was added during early log phase of bacterial growth at 37ºC, resulting the production of new intracellular proteins with similar approximate size of 180 kDa in which recognized as DNA-directed RNA polymerase β subunit enzyme and respiratory nitrate reductase α subunit enzyme respectively. Besides, when B. subtilis ATCC 21332 were induced by C. flexuosus essential oil and Streptomycin Sulfate during log phase of growing cells at 30ºC could also secrete the extracellular proteins with approximate size of 30 kDa, identified as Bacillopeptidase F. In addition, B. subtilis ATCC 21332 in the presence of either C. nardus or C. flexuosus essential oils could secrete the bioactive extracellular proteins with potent antimicrobial activity against certain Gram-positive and Gram-negative bacteria. Hence, B. subtilis ATCC 21332 in stressful condition with the presence of either Cymbopogon sp. essential oils or Streptomycin Sulfate at 0.01 MIC level were able to induce the production or secretion of bioactive proteins.
format Newspaper Article
author Hairul Shahril Bin Muhamad
author_facet Hairul Shahril Bin Muhamad
author_sort Hairul Shahril Bin Muhamad
title Effects Of Cymbopogon Sp. Essential Oils And Streptomycin Sulfate On Antimicrobial Proteins Production By Bacillus Subtilis Atcc 21332
title_short Effects Of Cymbopogon Sp. Essential Oils And Streptomycin Sulfate On Antimicrobial Proteins Production By Bacillus Subtilis Atcc 21332
title_full Effects Of Cymbopogon Sp. Essential Oils And Streptomycin Sulfate On Antimicrobial Proteins Production By Bacillus Subtilis Atcc 21332
title_fullStr Effects Of Cymbopogon Sp. Essential Oils And Streptomycin Sulfate On Antimicrobial Proteins Production By Bacillus Subtilis Atcc 21332
title_full_unstemmed Effects Of Cymbopogon Sp. Essential Oils And Streptomycin Sulfate On Antimicrobial Proteins Production By Bacillus Subtilis Atcc 21332
title_sort effects of cymbopogon sp. essential oils and streptomycin sulfate on antimicrobial proteins production by bacillus subtilis atcc 21332
publisher Universiti Sains Islam Malaysia
publishDate 2015
url http://ddms.usim.edu.my/handle/123456789/8629
_version_ 1645152450268626944
score 13.222552

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