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The improvement of in vivo model (Balb/c mice) for cervical carcinogenesis using diethylstilbestrol (DES)

Cervical cancer is the most common gynecological cancer and one of the major causes of female cancer-related death worldwide particularly in developing countries. Thus far, there are a few in vivo models have been developed in investigating this type of cancer. In this study, we induced cervical can...

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Main Authors: Latifah, Saiful Yazan,, Nurdin, Armania,, Said, Zulfahmi,, Hairuszah, Ithnin,
Format: Article
Language:en_US
Published: Elsevier Gmbh, Urban & Fischer Verlag 2015
Subjects:
In Vivo Model
Balb/C Mice
Diethylstilbestrol
Carcinogenesis
Adenosis
Interleukin-6
Online Access:http://ddms.usim.edu.my/handle/123456789/8448
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spelling my.usim-84482015-12-29T08:43:02Z The improvement of in vivo model (Balb/c mice) for cervical carcinogenesis using diethylstilbestrol (DES) Latifah, Saiful Yazan, Nurdin, Armania, Said, Zulfahmi, Hairuszah, Ithnin, In Vivo Model Balb/C Mice Diethylstilbestrol Carcinogenesis Adenosis Interleukin-6 Cervical cancer is the most common gynecological cancer and one of the major causes of female cancer-related death worldwide particularly in developing countries. Thus far, there are a few in vivo models have been developed in investigating this type of cancer. In this study, we induced cervical cancer in Balb/c mice by exploiting the carcinogenic property of diestylstilbestrol (DES). The Balb/c pregnant mice were given subcutaneous (SC) injection of 67 mu g/kg body weight of DES on GD 13, and the mice gave birth approximately at gestation day 19-22. Female offspring were reared and the body weight was recorded once weekly. The female offspring were sacrificed at age of 5 months. Upon termination, blood was collected in a plain tube via cardiac puncture and the reproductive tracts were collected and weighed. The reproductive tract sections were stained using H&E for observation of pathological changes. The progression of disease state was monitored by measuring the level of serum interleukin (IL-6) using the Mouse IL-6 ELISA Assay Kit (BD OptEIA(TM), USA). All parameters were compared with Not-induced group. The outcome of this study demonstrated a significant difference in body weight gain, reproductive organ weight, diameter, of cervix and the level of serum IL-6 in the Induced group as compared to the Not-induced group (P<0.05). Histopathological findings revealed the presence of adenosis only in the Induced group. It shows that DES could be employed as an agent to induce cervical carcinogenesis in animal model. In addition to that, new potential anti-cancer agents from various sources could be further evaluated using this technique. (C) 2013 Published by Elsevier GmbH. 2015-06-19T03:54:04Z 2015-06-19T03:54:04Z 2013-01-01 Article 0940-2993 1618-1433 http://ddms.usim.edu.my/handle/123456789/8448 en_US Elsevier Gmbh, Urban & Fischer Verlag
institution Universiti Sains Islam Malaysia
building USIM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universit Sains Islam i Malaysia
content_source USIM Institutional Repository
url_provider http://ddms.usim.edu.my/
language en_US
topic In Vivo Model
Balb/C Mice
Diethylstilbestrol
Carcinogenesis
Adenosis
Interleukin-6
spellingShingle In Vivo Model
Balb/C Mice
Diethylstilbestrol
Carcinogenesis
Adenosis
Interleukin-6
Latifah, Saiful Yazan,
Nurdin, Armania,
Said, Zulfahmi,
Hairuszah, Ithnin,
The improvement of in vivo model (Balb/c mice) for cervical carcinogenesis using diethylstilbestrol (DES)
description Cervical cancer is the most common gynecological cancer and one of the major causes of female cancer-related death worldwide particularly in developing countries. Thus far, there are a few in vivo models have been developed in investigating this type of cancer. In this study, we induced cervical cancer in Balb/c mice by exploiting the carcinogenic property of diestylstilbestrol (DES). The Balb/c pregnant mice were given subcutaneous (SC) injection of 67 mu g/kg body weight of DES on GD 13, and the mice gave birth approximately at gestation day 19-22. Female offspring were reared and the body weight was recorded once weekly. The female offspring were sacrificed at age of 5 months. Upon termination, blood was collected in a plain tube via cardiac puncture and the reproductive tracts were collected and weighed. The reproductive tract sections were stained using H&E for observation of pathological changes. The progression of disease state was monitored by measuring the level of serum interleukin (IL-6) using the Mouse IL-6 ELISA Assay Kit (BD OptEIA(TM), USA). All parameters were compared with Not-induced group. The outcome of this study demonstrated a significant difference in body weight gain, reproductive organ weight, diameter, of cervix and the level of serum IL-6 in the Induced group as compared to the Not-induced group (P<0.05). Histopathological findings revealed the presence of adenosis only in the Induced group. It shows that DES could be employed as an agent to induce cervical carcinogenesis in animal model. In addition to that, new potential anti-cancer agents from various sources could be further evaluated using this technique. (C) 2013 Published by Elsevier GmbH.
format Article
author Latifah, Saiful Yazan,
Nurdin, Armania,
Said, Zulfahmi,
Hairuszah, Ithnin,
author_facet Latifah, Saiful Yazan,
Nurdin, Armania,
Said, Zulfahmi,
Hairuszah, Ithnin,
author_sort Latifah, Saiful Yazan,
title The improvement of in vivo model (Balb/c mice) for cervical carcinogenesis using diethylstilbestrol (DES)
title_short The improvement of in vivo model (Balb/c mice) for cervical carcinogenesis using diethylstilbestrol (DES)
title_full The improvement of in vivo model (Balb/c mice) for cervical carcinogenesis using diethylstilbestrol (DES)
title_fullStr The improvement of in vivo model (Balb/c mice) for cervical carcinogenesis using diethylstilbestrol (DES)
title_full_unstemmed The improvement of in vivo model (Balb/c mice) for cervical carcinogenesis using diethylstilbestrol (DES)
title_sort improvement of in vivo model (balb/c mice) for cervical carcinogenesis using diethylstilbestrol (des)
publisher Elsevier Gmbh, Urban & Fischer Verlag
publishDate 2015
url http://ddms.usim.edu.my/handle/123456789/8448
_version_ 1645152419348217856
score 13.222552

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