Staff View: Temperature-regulated expression of outer membrane proteins in Shigella flexneri

Temperature-regulated expression of outer membrane proteins in Shigella flexneri

Background: Bacteria exist widely in a diversity of natural environments. In order to survive adverse conditions such as nutrient depletion, biochemical and biological disturbances, and high temperature, bacteria have developed a wide variety of coping mechanisms. Temperature is one of the most impo...

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Main Authors:	Hemavathy, Harikrishnan,, Kirnpal-Kaur Banga, Singh,, Asma, Ismail,
Format:	Article
Language:	en_US
Published:	Biomed Central Ltd 2015
Subjects:	Dna-Binding Protein Escherichia-Coli Oxidative Stress Salmonella-Typhi Stationary-Phase Virulence Gene Dps Aminopeptidase Identification
Online Access:	http://ddms.usim.edu.my/handle/123456789/8437 http://download.springer.com/static/pdf/388/art%253A10.1186%252F1757-4749-5-38.pdf?originUrl=http%3A%2F%2Fgutpathogens.biomedcentral.com%2Farticle%2F10.1186%2F1757-4749-5-38&token2=exp=1451532097~acl=%2Fstatic%2Fpdf%2F388%2Fart%25253A10.1186%25252F1757-4749-5-38.pdf*~hmac=a40f19bee4d59b1440be2ebd406581c6b892ebce93bfe0a7a136d0129d7ff531
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spelling	my.usim-84372015-12-31T03:17:20Z Temperature-regulated expression of outer membrane proteins in Shigella flexneri Hemavathy, Harikrishnan, Kirnpal-Kaur Banga, Singh, Asma, Ismail, Dna-Binding Protein Escherichia-Coli Oxidative Stress Salmonella-Typhi Stationary-Phase Virulence Gene Dps Aminopeptidase Identification Background: Bacteria exist widely in a diversity of natural environments. In order to survive adverse conditions such as nutrient depletion, biochemical and biological disturbances, and high temperature, bacteria have developed a wide variety of coping mechanisms. Temperature is one of the most important factors that can enhance the expression of microbial proteins. This study was conducted to investigate how outer membrane proteins (OMPs) of the bacterium Shigella flexneri respond to stress, especially during fever when the host's body temperature is elevated. Methods: OMPs of S. flexneri ATCC 12022 and clinical isolate SH057 were extracted from an overnight culture grown at 37, 38.5, and 40 degrees C. Comparisons of the expressed proteins under the different growth conditions were based on equal numbers of bacterial cells loaded in the SDS-PAGE gels. Separated proteins were stained with Coomassie brilliant blue. Selected proteins showing increased expression at 38.5 and 40 degrees C were characterized by performing MALDI-ToF-ToF. Results: Different degrees of expression were demonstrated for different proteins expressed at 37 degrees C compared to 38.5 and 40 degrees C. The proteins with molecular sizes of 18.4, 25.6, and 57.0 kDa showed increased expression level at increasing temperature and were identified as Dps, WrbA, and PepA, respectively. Conclusion: This study revealed that strains of S. flexneri respond at the proteomic level during stress caused by elevated temperature by decreasing the expression of proteins, maintaining the level of important proteins, or enhancing the levels of proteins presumably involved in survival and virulence. 2015-06-19T02:53:50Z 2015-06-19T02:53:50Z 2013 Article 1757-4749 http://ddms.usim.edu.my/handle/123456789/8437 http://download.springer.com/static/pdf/388/art%253A10.1186%252F1757-4749-5-38.pdf?originUrl=http%3A%2F%2Fgutpathogens.biomedcentral.com%2Farticle%2F10.1186%2F1757-4749-5-38&token2=exp=1451532097~acl=%2Fstatic%2Fpdf%2F388%2Fart%25253A10.1186%25252F1757-4749-5-38.pdf*~hmac=a40f19bee4d59b1440be2ebd406581c6b892ebce93bfe0a7a136d0129d7ff531 en_US Biomed Central Ltd
institution	Universiti Sains Islam Malaysia
building	USIM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universit Sains Islam i Malaysia
content_source	USIM Institutional Repository
url_provider	http://ddms.usim.edu.my/
language	en_US
topic	Dna-Binding Protein Escherichia-Coli Oxidative Stress Salmonella-Typhi Stationary-Phase Virulence Gene Dps Aminopeptidase Identification
spellingShingle	Dna-Binding Protein Escherichia-Coli Oxidative Stress Salmonella-Typhi Stationary-Phase Virulence Gene Dps Aminopeptidase Identification Hemavathy, Harikrishnan, Kirnpal-Kaur Banga, Singh, Asma, Ismail, Temperature-regulated expression of outer membrane proteins in Shigella flexneri
description	Background: Bacteria exist widely in a diversity of natural environments. In order to survive adverse conditions such as nutrient depletion, biochemical and biological disturbances, and high temperature, bacteria have developed a wide variety of coping mechanisms. Temperature is one of the most important factors that can enhance the expression of microbial proteins. This study was conducted to investigate how outer membrane proteins (OMPs) of the bacterium Shigella flexneri respond to stress, especially during fever when the host's body temperature is elevated. Methods: OMPs of S. flexneri ATCC 12022 and clinical isolate SH057 were extracted from an overnight culture grown at 37, 38.5, and 40 degrees C. Comparisons of the expressed proteins under the different growth conditions were based on equal numbers of bacterial cells loaded in the SDS-PAGE gels. Separated proteins were stained with Coomassie brilliant blue. Selected proteins showing increased expression at 38.5 and 40 degrees C were characterized by performing MALDI-ToF-ToF. Results: Different degrees of expression were demonstrated for different proteins expressed at 37 degrees C compared to 38.5 and 40 degrees C. The proteins with molecular sizes of 18.4, 25.6, and 57.0 kDa showed increased expression level at increasing temperature and were identified as Dps, WrbA, and PepA, respectively. Conclusion: This study revealed that strains of S. flexneri respond at the proteomic level during stress caused by elevated temperature by decreasing the expression of proteins, maintaining the level of important proteins, or enhancing the levels of proteins presumably involved in survival and virulence.
format	Article
author	Hemavathy, Harikrishnan, Kirnpal-Kaur Banga, Singh, Asma, Ismail,
author_facet	Hemavathy, Harikrishnan, Kirnpal-Kaur Banga, Singh, Asma, Ismail,
author_sort	Hemavathy, Harikrishnan,
title	Temperature-regulated expression of outer membrane proteins in Shigella flexneri
title_short	Temperature-regulated expression of outer membrane proteins in Shigella flexneri
title_full	Temperature-regulated expression of outer membrane proteins in Shigella flexneri
title_fullStr	Temperature-regulated expression of outer membrane proteins in Shigella flexneri
title_full_unstemmed	Temperature-regulated expression of outer membrane proteins in Shigella flexneri
title_sort	temperature-regulated expression of outer membrane proteins in shigella flexneri
publisher	Biomed Central Ltd
publishDate	2015
url	http://ddms.usim.edu.my/handle/123456789/8437 http://download.springer.com/static/pdf/388/art%253A10.1186%252F1757-4749-5-38.pdf?originUrl=http%3A%2F%2Fgutpathogens.biomedcentral.com%2Farticle%2F10.1186%2F1757-4749-5-38&token2=exp=1451532097~acl=%2Fstatic%2Fpdf%2F388%2Fart%25253A10.1186%25252F1757-4749-5-38.pdf*~hmac=a40f19bee4d59b1440be2ebd406581c6b892ebce93bfe0a7a136d0129d7ff531
_version_	1645152417538375680
score	13.222552

Temperature-regulated expression of outer membrane proteins in Shigella flexneri

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