Staff View: Pro-angiogenic potential of human chorion-derived stem cells: in vitro and in vivo evaluation

Pro-angiogenic potential of human chorion-derived stem cells: in vitro and in vivo evaluation

Human chorion-derived stem cells (hCDSC) were previously shown to demonstrate multipotent properties with promising angiogenic characteristics in monolayer-cell culture system. In our study, we investigated the angiogenic capability of hCDSC in 3-dimensional (3D) in vitro and in vivo angiogenic mode...

Full description

Saved in:

Bibliographic Details
Main Authors:	Geok-Chin, Tan,, Kien-Hui, Chua,, Yun-Hsuen, Lim,, Mohd-Manzor, N.Fariha,, Abdul-Rahman, Hayati,
Format:	Article
Language:	English
Published:	Wiley-Blackwell 2015
Subjects:	Fetal Stem Cells Pro-Angiogenic Quantitative PCR Vascular Ischemic Disease
Online Access:	http://ddms.usim.edu.my/handle/123456789/8383
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my.usim-8383
record_format	dspace
spelling	my.usim-83832017-02-20T04:40:10Z Pro-angiogenic potential of human chorion-derived stem cells: in vitro and in vivo evaluation Geok-Chin, Tan, Kien-Hui, Chua, Yun-Hsuen, Lim, Mohd-Manzor, N.Fariha, Abdul-Rahman, Hayati, Fetal Stem Cells Pro-Angiogenic Quantitative PCR Vascular Ischemic Disease Human chorion-derived stem cells (hCDSC) were previously shown to demonstrate multipotent properties with promising angiogenic characteristics in monolayer-cell culture system. In our study, we investigated the angiogenic capability of hCDSC in 3-dimensional (3D) in vitro and in vivo angiogenic models for the purpose of future application in the treatment of ischaemic diseases. Human CDSC were evaluated for angiogenic and endogenic genes expressions by quantitative PCR. Growth factors secretions were quantified using ELISA. In vitro and in vivo vascular formations were evaluated by histological analysis and confocal microscopic imaging. PECAM-1+ and vWF+ vascular-like structures were observed in both in vitro and in vivo angiogenesis models. High secretions of VEGF and bFGF by hCDSC with increased expressions of angiogenic and endogenic genes suggested the possible angiogenic promoting mechanisms by hCDSC. The cooperation of hCDSC with HUVECS to generate vessel-like structures in our systems is an indication that there will be positive interactions of hCDSC with existing endothelial cells when injected into ischaemic tissues. Hence, hCDSC is suggested as the novel approach in the future treatment of ischaemic diseases. 2015-06-16T03:27:26Z 2015-06-16T03:27:26Z 2013 Article 1582-4934 http://ddms.usim.edu.my/handle/123456789/8383 en Wiley-Blackwell
institution	Universiti Sains Islam Malaysia
building	USIM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universit Sains Islam i Malaysia
content_source	USIM Institutional Repository
url_provider	http://ddms.usim.edu.my/
language	English
topic	Fetal Stem Cells Pro-Angiogenic Quantitative PCR Vascular Ischemic Disease
spellingShingle	Fetal Stem Cells Pro-Angiogenic Quantitative PCR Vascular Ischemic Disease Geok-Chin, Tan, Kien-Hui, Chua, Yun-Hsuen, Lim, Mohd-Manzor, N.Fariha, Abdul-Rahman, Hayati, Pro-angiogenic potential of human chorion-derived stem cells: in vitro and in vivo evaluation
description	Human chorion-derived stem cells (hCDSC) were previously shown to demonstrate multipotent properties with promising angiogenic characteristics in monolayer-cell culture system. In our study, we investigated the angiogenic capability of hCDSC in 3-dimensional (3D) in vitro and in vivo angiogenic models for the purpose of future application in the treatment of ischaemic diseases. Human CDSC were evaluated for angiogenic and endogenic genes expressions by quantitative PCR. Growth factors secretions were quantified using ELISA. In vitro and in vivo vascular formations were evaluated by histological analysis and confocal microscopic imaging. PECAM-1+ and vWF+ vascular-like structures were observed in both in vitro and in vivo angiogenesis models. High secretions of VEGF and bFGF by hCDSC with increased expressions of angiogenic and endogenic genes suggested the possible angiogenic promoting mechanisms by hCDSC. The cooperation of hCDSC with HUVECS to generate vessel-like structures in our systems is an indication that there will be positive interactions of hCDSC with existing endothelial cells when injected into ischaemic tissues. Hence, hCDSC is suggested as the novel approach in the future treatment of ischaemic diseases.
format	Article
author	Geok-Chin, Tan, Kien-Hui, Chua, Yun-Hsuen, Lim, Mohd-Manzor, N.Fariha, Abdul-Rahman, Hayati,
author_facet	Geok-Chin, Tan, Kien-Hui, Chua, Yun-Hsuen, Lim, Mohd-Manzor, N.Fariha, Abdul-Rahman, Hayati,
author_sort	Geok-Chin, Tan,
title	Pro-angiogenic potential of human chorion-derived stem cells: in vitro and in vivo evaluation
title_short	Pro-angiogenic potential of human chorion-derived stem cells: in vitro and in vivo evaluation
title_full	Pro-angiogenic potential of human chorion-derived stem cells: in vitro and in vivo evaluation
title_fullStr	Pro-angiogenic potential of human chorion-derived stem cells: in vitro and in vivo evaluation
title_full_unstemmed	Pro-angiogenic potential of human chorion-derived stem cells: in vitro and in vivo evaluation
title_sort	pro-angiogenic potential of human chorion-derived stem cells: in vitro and in vivo evaluation
publisher	Wiley-Blackwell
publishDate	2015
url	http://ddms.usim.edu.my/handle/123456789/8383
_version_	1645152406657302528
score	13.222552

Pro-angiogenic potential of human chorion-derived stem cells: in vitro and in vivo evaluation

Similar Items