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Kinetic Behaviour of Free Lipase and Mica-Based Immobilized Lipase Catalyzing the Synthesis of Sugar Esters

The utilization of natural mica as a biocatalyst support in kinetic investigations is first described in this study. The formation of lactose caprate from lactose sugar and capric acid, using free lipase (free-CRL) and lipase immobilized on nanoporous mica (NER-CRL) as a biocatalyst, was evaluated t...

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Main Authors: Uswatun Hasanah, Zaidan,, Mohd Basyaruddin, Abdul Rahman,, Mahiran, Basri,, Emilia, Abdulmalek,, Siti Salhah, Othman,, Raja Noor Zaliha, Raja Abd Rahman,, Abu Bakar, Salleh,
Format: Article
Language:English
Published: Taylor & Francis Ltd 2015
Subjects:
Sugar Ester Synthesis
Lipase Immobilization
Mica
Kinetic Study
Ping-Pong Bi-Bi Model
Online Access:http://ddms.usim.edu.my/handle/123456789/8298
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spelling my.usim-82982015-12-29T03:15:18Z Kinetic Behaviour of Free Lipase and Mica-Based Immobilized Lipase Catalyzing the Synthesis of Sugar Esters Uswatun Hasanah, Zaidan, Mohd Basyaruddin, Abdul Rahman, Mahiran, Basri, Emilia, Abdulmalek, Siti Salhah, Othman, Raja Noor Zaliha, Raja Abd Rahman, Abu Bakar, Salleh, Sugar Ester Synthesis Lipase Immobilization Mica Kinetic Study Ping-Pong Bi-Bi Model The utilization of natural mica as a biocatalyst support in kinetic investigations is first described in this study. The formation of lactose caprate from lactose sugar and capric acid, using free lipase (free-CRL) and lipase immobilized on nanoporous mica (NER-CRL) as a biocatalyst, was evaluated through a kinetic study. The apparent kinetic parameters, K-m and V-max, were determined by means of the Michaelis-Menten kinetic model. The Ping-Pong Bi-Bi mechanism with single substrate inhibition was adopted as it best explains the experimental findings. The kinetic results show lower K-m values with NER-CRL than with free-CRL, indicating the higher affinity of NER-CRL towards both substrates at the maximum reaction velocity V-max,V-app > V-max). The kinetic parameters deduced from this model were used to simulate reaction rate data which were in close agreement with the experimental values. 2015-06-10T04:53:44Z 2015-06-10T04:53:44Z 2011-01-01 Article 0916-8451 1347-6947 http://ddms.usim.edu.my/handle/123456789/8298 https://www.jstage.jst.go.jp/article/bbb/75/8/75_110117/_pdf en Taylor & Francis Ltd
institution Universiti Sains Islam Malaysia
building USIM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universit Sains Islam i Malaysia
content_source USIM Institutional Repository
url_provider http://ddms.usim.edu.my/
language English
topic Sugar Ester Synthesis
Lipase Immobilization
Mica
Kinetic Study
Ping-Pong Bi-Bi Model
spellingShingle Sugar Ester Synthesis
Lipase Immobilization
Mica
Kinetic Study
Ping-Pong Bi-Bi Model
Uswatun Hasanah, Zaidan,
Mohd Basyaruddin, Abdul Rahman,
Mahiran, Basri,
Emilia, Abdulmalek,
Siti Salhah, Othman,
Raja Noor Zaliha, Raja Abd Rahman,
Abu Bakar, Salleh,
Kinetic Behaviour of Free Lipase and Mica-Based Immobilized Lipase Catalyzing the Synthesis of Sugar Esters
description The utilization of natural mica as a biocatalyst support in kinetic investigations is first described in this study. The formation of lactose caprate from lactose sugar and capric acid, using free lipase (free-CRL) and lipase immobilized on nanoporous mica (NER-CRL) as a biocatalyst, was evaluated through a kinetic study. The apparent kinetic parameters, K-m and V-max, were determined by means of the Michaelis-Menten kinetic model. The Ping-Pong Bi-Bi mechanism with single substrate inhibition was adopted as it best explains the experimental findings. The kinetic results show lower K-m values with NER-CRL than with free-CRL, indicating the higher affinity of NER-CRL towards both substrates at the maximum reaction velocity V-max,V-app > V-max). The kinetic parameters deduced from this model were used to simulate reaction rate data which were in close agreement with the experimental values.
format Article
author Uswatun Hasanah, Zaidan,
Mohd Basyaruddin, Abdul Rahman,
Mahiran, Basri,
Emilia, Abdulmalek,
Siti Salhah, Othman,
Raja Noor Zaliha, Raja Abd Rahman,
Abu Bakar, Salleh,
author_facet Uswatun Hasanah, Zaidan,
Mohd Basyaruddin, Abdul Rahman,
Mahiran, Basri,
Emilia, Abdulmalek,
Siti Salhah, Othman,
Raja Noor Zaliha, Raja Abd Rahman,
Abu Bakar, Salleh,
author_sort Uswatun Hasanah, Zaidan,
title Kinetic Behaviour of Free Lipase and Mica-Based Immobilized Lipase Catalyzing the Synthesis of Sugar Esters
title_short Kinetic Behaviour of Free Lipase and Mica-Based Immobilized Lipase Catalyzing the Synthesis of Sugar Esters
title_full Kinetic Behaviour of Free Lipase and Mica-Based Immobilized Lipase Catalyzing the Synthesis of Sugar Esters
title_fullStr Kinetic Behaviour of Free Lipase and Mica-Based Immobilized Lipase Catalyzing the Synthesis of Sugar Esters
title_full_unstemmed Kinetic Behaviour of Free Lipase and Mica-Based Immobilized Lipase Catalyzing the Synthesis of Sugar Esters
title_sort kinetic behaviour of free lipase and mica-based immobilized lipase catalyzing the synthesis of sugar esters
publisher Taylor & Francis Ltd
publishDate 2015
url http://ddms.usim.edu.my/handle/123456789/8298
_version_ 1645152388072341504
score 13.222552

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