Staff View: In-house developed light cycler real time polymerase chain reaction for rapid detection of respiratory syncytial virus at University Kebangsaan Medical Centre, Malaysia

In-house developed light cycler real time polymerase chain reaction for rapid detection of respiratory syncytial virus at University Kebangsaan Medical Centre, Malaysia

Objectives: Respiratory syncytial virus (RSV) is a major cause of respiratory illness in young children worldwide. Its detection by viral culture and immunofluorescent assay is time consuming and has low sensitivity. The objective of the study was to develop In House Light Cycler Real Time Polymeras...

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Main Authors:	Wong Kon, Ken,, M. M., Rahman,, Alfizah, Hanafiah,, Ilina, Isahak,
Format:	Article
Language:	English
Published:	Professional Medical Publications 2015
Subjects:	Sequence Probe And Plasmid Respiratory Syncytial Virus Lightcycler Real Time Polymerase Chain Reaction Primer
Online Access:	http://ddms.usim.edu.my/handle/123456789/8295
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spelling	my.usim-82952017-02-23T03:48:53Z In-house developed light cycler real time polymerase chain reaction for rapid detection of respiratory syncytial virus at University Kebangsaan Medical Centre, Malaysia Wong Kon, Ken, M. M., Rahman, Alfizah, Hanafiah, Ilina, Isahak, Sequence Probe And Plasmid Respiratory Syncytial Virus Lightcycler Real Time Polymerase Chain Reaction Primer Objectives: Respiratory syncytial virus (RSV) is a major cause of respiratory illness in young children worldwide. Its detection by viral culture and immunofluorescent assay is time consuming and has low sensitivity. The objective of the study was to develop In House Light Cycler Real Time Polymerase Chain Reaction for Rapid Detection of Respiratory Syncytial Virus. Methodology: In house developed Light Cycler Real-Time reverse transcriptase polymerase chain reaction (LC-RT-PCR) assay was standardized using designed primers for the rapid identification of clinical samples of RSV. Results: A total of 130 samples (throat swab and nasopharyngeal aspirate) obtained from University Kebangsaan Malaysia Medical Centre, were efficiently tested and of which 54 (42%) were RSV positive. Conclusion: This study indicated that this newly developed in house rRT-PCR assay proved to be an excellent method for the rapid detection of RSV. 2015-06-10T04:41:52Z 2015-06-10T04:41:52Z 2012 Article 1682-024X http://ddms.usim.edu.my/handle/123456789/8295 en Professional Medical Publications
institution	Universiti Sains Islam Malaysia
building	USIM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universit Sains Islam i Malaysia
content_source	USIM Institutional Repository
url_provider	http://ddms.usim.edu.my/
language	English
topic	Sequence Probe And Plasmid Respiratory Syncytial Virus Lightcycler Real Time Polymerase Chain Reaction Primer
spellingShingle	Sequence Probe And Plasmid Respiratory Syncytial Virus Lightcycler Real Time Polymerase Chain Reaction Primer Wong Kon, Ken, M. M., Rahman, Alfizah, Hanafiah, Ilina, Isahak, In-house developed light cycler real time polymerase chain reaction for rapid detection of respiratory syncytial virus at University Kebangsaan Medical Centre, Malaysia
description	Objectives: Respiratory syncytial virus (RSV) is a major cause of respiratory illness in young children worldwide. Its detection by viral culture and immunofluorescent assay is time consuming and has low sensitivity. The objective of the study was to develop In House Light Cycler Real Time Polymerase Chain Reaction for Rapid Detection of Respiratory Syncytial Virus. Methodology: In house developed Light Cycler Real-Time reverse transcriptase polymerase chain reaction (LC-RT-PCR) assay was standardized using designed primers for the rapid identification of clinical samples of RSV. Results: A total of 130 samples (throat swab and nasopharyngeal aspirate) obtained from University Kebangsaan Malaysia Medical Centre, were efficiently tested and of which 54 (42%) were RSV positive. Conclusion: This study indicated that this newly developed in house rRT-PCR assay proved to be an excellent method for the rapid detection of RSV.
format	Article
author	Wong Kon, Ken, M. M., Rahman, Alfizah, Hanafiah, Ilina, Isahak,
author_facet	Wong Kon, Ken, M. M., Rahman, Alfizah, Hanafiah, Ilina, Isahak,
author_sort	Wong Kon, Ken,
title	In-house developed light cycler real time polymerase chain reaction for rapid detection of respiratory syncytial virus at University Kebangsaan Medical Centre, Malaysia
title_short	In-house developed light cycler real time polymerase chain reaction for rapid detection of respiratory syncytial virus at University Kebangsaan Medical Centre, Malaysia
title_full	In-house developed light cycler real time polymerase chain reaction for rapid detection of respiratory syncytial virus at University Kebangsaan Medical Centre, Malaysia
title_fullStr	In-house developed light cycler real time polymerase chain reaction for rapid detection of respiratory syncytial virus at University Kebangsaan Medical Centre, Malaysia
title_full_unstemmed	In-house developed light cycler real time polymerase chain reaction for rapid detection of respiratory syncytial virus at University Kebangsaan Medical Centre, Malaysia
title_sort	in-house developed light cycler real time polymerase chain reaction for rapid detection of respiratory syncytial virus at university kebangsaan medical centre, malaysia
publisher	Professional Medical Publications
publishDate	2015
url	http://ddms.usim.edu.my/handle/123456789/8295
_version_	1645152387487236096
score	13.211869

In-house developed light cycler real time polymerase chain reaction for rapid detection of respiratory syncytial virus at University Kebangsaan Medical Centre, Malaysia

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