Effects of Mg2+, Fe3+, Mn2+ and Cu2+ Ions on Lipid Accumulation by Cunninghamella bainieri 2A1
Cunninghamella bainieri 2A1 is an oleaginous fungus whose lipid accumulation profile is significantly influenced by metal ion concentrations in growth medium. Mg2+, Fe3+, Mn2+ and Cu2+ were found to be the important elements affecting lipid accumulation in this fungus. This study employs a statistic...
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2015
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my.usim-81252017-02-23T04:39:16Z Effects of Mg2+, Fe3+, Mn2+ and Cu2+ Ions on Lipid Accumulation by Cunninghamella bainieri 2A1 Vidyah, Manikan, Othman, Omar, Aidil, Abdul Hamid, Mohd Sahaid, Kalil, Abdul Jalil, Abdul Kader, Cunninghamella Metal Ions Response Surface Methodology Single Cell Oil Cunninghamella bainieri 2A1 is an oleaginous fungus whose lipid accumulation profile is significantly influenced by metal ion concentrations in growth medium. Mg2+, Fe3+, Mn2+ and Cu2+ were found to be the important elements affecting lipid accumulation in this fungus. This study employs a statistical method (Response Surface Methodology -RSM) to study the combined effects of Mg2+, Fe3+, Mn2+ and Cu2+ on lipid accumulation of C. baineri 2A1. Cultivation was carried out in 250 mL Erlenmeyer flasks containing 100 mL nitrogen limited medium at 30 degrees C and 250 rpm agitation for 120 h. A thirty-run central composite design experiment was employed to identify and optimize the significant factors. In addition to Mg2(+) and Fe3(+) which were shown to have significant effects on lipid accumulation, the interactions between Mg2+ and Cu2+, as well as the effect of Cu2+ in quadratic terms were also found to have significant effect on the process (p< 0.05). The highest amount of lipid obtained in this study was 39% g/g biomass with optimal levels of Mg2+, Fe3+ and Cu2+ at 5.00, 0.017 and 0.0005 g/L, respectively, while Mn2+ was omitted. A 32% increment in lipid yield was recorded, where the lipid content increased to 38%, compared to initial yield of 29% g/g biomass prior to optimization. In conclusion, Mg2+ and Fe3+ have significant positive effect on the lipid accumulation of this fungus, whereas Mn2+ and Cu2+ exert negative effects in combination. 2015-05-18T05:02:43Z 2015-05-18T05:02:43Z 2014 Article 0126-6039 http://ddms.usim.edu.my/handle/123456789/8125 en Universiti Kebangsaan Malaysia |
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Cunninghamella Metal Ions Response Surface Methodology Single Cell Oil |
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Cunninghamella Metal Ions Response Surface Methodology Single Cell Oil Vidyah, Manikan, Othman, Omar, Aidil, Abdul Hamid, Mohd Sahaid, Kalil, Abdul Jalil, Abdul Kader, Effects of Mg2+, Fe3+, Mn2+ and Cu2+ Ions on Lipid Accumulation by Cunninghamella bainieri 2A1 |
| description |
Cunninghamella bainieri 2A1 is an oleaginous fungus whose lipid accumulation profile is significantly influenced by metal ion concentrations in growth medium. Mg2+, Fe3+, Mn2+ and Cu2+ were found to be the important elements affecting lipid accumulation in this fungus. This study employs a statistical method (Response Surface Methodology -RSM) to study the combined effects of Mg2+, Fe3+, Mn2+ and Cu2+ on lipid accumulation of C. baineri 2A1. Cultivation was carried out in 250 mL Erlenmeyer flasks containing 100 mL nitrogen limited medium at 30 degrees C and 250 rpm agitation for 120 h. A thirty-run central composite design experiment was employed to identify and optimize the significant factors. In addition to Mg2(+) and Fe3(+) which were shown to have significant effects on lipid accumulation, the interactions between Mg2+ and Cu2+, as well as the effect of Cu2+ in quadratic terms were also found to have significant effect on the process (p< 0.05). The highest amount of lipid obtained in this study was 39% g/g biomass with optimal levels of Mg2+, Fe3+ and Cu2+ at 5.00, 0.017 and 0.0005 g/L, respectively, while Mn2+ was omitted. A 32% increment in lipid yield was recorded, where the lipid content increased to 38%, compared to initial yield of 29% g/g biomass prior to optimization. In conclusion, Mg2+ and Fe3+ have significant positive effect on the lipid accumulation of this fungus, whereas Mn2+ and Cu2+ exert negative effects in combination. |
| format |
Article |
| author |
Vidyah, Manikan, Othman, Omar, Aidil, Abdul Hamid, Mohd Sahaid, Kalil, Abdul Jalil, Abdul Kader, |
| author_facet |
Vidyah, Manikan, Othman, Omar, Aidil, Abdul Hamid, Mohd Sahaid, Kalil, Abdul Jalil, Abdul Kader, |
| author_sort |
Vidyah, Manikan, |
| title |
Effects of Mg2+, Fe3+, Mn2+ and Cu2+ Ions on Lipid Accumulation by Cunninghamella bainieri 2A1 |
| title_short |
Effects of Mg2+, Fe3+, Mn2+ and Cu2+ Ions on Lipid Accumulation by Cunninghamella bainieri 2A1 |
| title_full |
Effects of Mg2+, Fe3+, Mn2+ and Cu2+ Ions on Lipid Accumulation by Cunninghamella bainieri 2A1 |
| title_fullStr |
Effects of Mg2+, Fe3+, Mn2+ and Cu2+ Ions on Lipid Accumulation by Cunninghamella bainieri 2A1 |
| title_full_unstemmed |
Effects of Mg2+, Fe3+, Mn2+ and Cu2+ Ions on Lipid Accumulation by Cunninghamella bainieri 2A1 |
| title_sort |
effects of mg2+, fe3+, mn2+ and cu2+ ions on lipid accumulation by cunninghamella bainieri 2a1 |
| publisher |
Universiti Kebangsaan Malaysia |
| publishDate |
2015 |
| url |
http://ddms.usim.edu.my/handle/123456789/8125 |
| _version_ |
1645152344330993664 |
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13.222552 |