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Effects of epidermal growth factor on the proliferation and cell cycle regulation of cultured human amnion epithelial cells

Human amnion epithelial cells (HAECs) hold great promise in tissue engineering for regenerative medicine. Large numbers of HAECs are required for this purpose. Hence, exogenous growth factor is added to the culture medium to improve epithelial cells proliferation. The aim of the present study was to...

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Main Authors: Abdul Rahman, Hayati,, Simat Siti, Fatimah,, Geok Chin, Tan,, Kien Hui, Chua,, Ay Eeng, Tan,
Format: Article
Language:English
Published: Soc Bioscience Bioengineering Japan 2015
Subjects:
Epidermal Growth Factor
Human Amnion
Epithelial
Pluripotent
Cell Cycle
Tissue Engineering
Online Access:http://ddms.usim.edu.my/handle/123456789/8122
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spelling my.usim-81222017-02-20T04:31:36Z Effects of epidermal growth factor on the proliferation and cell cycle regulation of cultured human amnion epithelial cells Abdul Rahman, Hayati, Simat Siti, Fatimah, Geok Chin, Tan, Kien Hui, Chua, Ay Eeng, Tan, Epidermal Growth Factor Human Amnion Epithelial Pluripotent Cell Cycle Tissue Engineering Human amnion epithelial cells (HAECs) hold great promise in tissue engineering for regenerative medicine. Large numbers of HAECs are required for this purpose. Hence, exogenous growth factor is added to the culture medium to improve epithelial cells proliferation. The aim of the present study was to determine the effects of epidermal growth factor (EGF) on the proliferation and cell cycle regulation of cultured HAECs. HAECs at P1 were cultured for 7 days in medium containing an equal volume mix of HAM's F12: Dulbecco's Modified Eagles Medium (1:1) supplemented with different concentrations of EGF (0, 5, 10, 20, 30 and 50 ng/ml EGF) in reduced serum. Morphology, growth kinetics and cell cycle analysis using flow cytometry were assessed. Quantitative gene expression for cell cycle control genes, pluripotent transcription factors, epithelial genes and neuronal genes were also determined. EGF enhanced HAECs proliferation with optimal concentration at 10 ng/ml EGF. EGF significantly increased the proportion of HAECs at S- and G2/M-phase of the cell cycle compared to the control. At the end of culture, HAECs remained as diploid cells under cell cycle analysis. EGF significantly decreased the mRNA expression of p21, pRb, p53 and GADD45 in cultured HAECs. EGF also significantly decreased the pluripotent genes expression: Oct-3/4, Sox2 and Nanog; epithelial genes expression: CK14, p63, CK1 and Involucrin; and neuronal gene expression: NSE, NF-M and MAP 2. The results suggested that EGF is a strong mitogen that promotes the proliferation of HAECs through cell cycle regulation. EGF did not promote HAECs differentiation or pluripotent genes expression. (c) 2012, The Society for Biotechnology, Japan. All rights reserved. 2015-05-18T04:51:36Z 2015-05-18T04:51:36Z 2012 Article 1389-1723 http://ddms.usim.edu.my/handle/123456789/8122 en Soc Bioscience Bioengineering Japan
institution Universiti Sains Islam Malaysia
building USIM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universit Sains Islam i Malaysia
content_source USIM Institutional Repository
url_provider http://ddms.usim.edu.my/
language English
topic Epidermal Growth Factor
Human Amnion
Epithelial
Pluripotent
Cell Cycle
Tissue Engineering
spellingShingle Epidermal Growth Factor
Human Amnion
Epithelial
Pluripotent
Cell Cycle
Tissue Engineering
Abdul Rahman, Hayati,
Simat Siti, Fatimah,
Geok Chin, Tan,
Kien Hui, Chua,
Ay Eeng, Tan,
Effects of epidermal growth factor on the proliferation and cell cycle regulation of cultured human amnion epithelial cells
description Human amnion epithelial cells (HAECs) hold great promise in tissue engineering for regenerative medicine. Large numbers of HAECs are required for this purpose. Hence, exogenous growth factor is added to the culture medium to improve epithelial cells proliferation. The aim of the present study was to determine the effects of epidermal growth factor (EGF) on the proliferation and cell cycle regulation of cultured HAECs. HAECs at P1 were cultured for 7 days in medium containing an equal volume mix of HAM's F12: Dulbecco's Modified Eagles Medium (1:1) supplemented with different concentrations of EGF (0, 5, 10, 20, 30 and 50 ng/ml EGF) in reduced serum. Morphology, growth kinetics and cell cycle analysis using flow cytometry were assessed. Quantitative gene expression for cell cycle control genes, pluripotent transcription factors, epithelial genes and neuronal genes were also determined. EGF enhanced HAECs proliferation with optimal concentration at 10 ng/ml EGF. EGF significantly increased the proportion of HAECs at S- and G2/M-phase of the cell cycle compared to the control. At the end of culture, HAECs remained as diploid cells under cell cycle analysis. EGF significantly decreased the mRNA expression of p21, pRb, p53 and GADD45 in cultured HAECs. EGF also significantly decreased the pluripotent genes expression: Oct-3/4, Sox2 and Nanog; epithelial genes expression: CK14, p63, CK1 and Involucrin; and neuronal gene expression: NSE, NF-M and MAP 2. The results suggested that EGF is a strong mitogen that promotes the proliferation of HAECs through cell cycle regulation. EGF did not promote HAECs differentiation or pluripotent genes expression. (c) 2012, The Society for Biotechnology, Japan. All rights reserved.
format Article
author Abdul Rahman, Hayati,
Simat Siti, Fatimah,
Geok Chin, Tan,
Kien Hui, Chua,
Ay Eeng, Tan,
author_facet Abdul Rahman, Hayati,
Simat Siti, Fatimah,
Geok Chin, Tan,
Kien Hui, Chua,
Ay Eeng, Tan,
author_sort Abdul Rahman, Hayati,
title Effects of epidermal growth factor on the proliferation and cell cycle regulation of cultured human amnion epithelial cells
title_short Effects of epidermal growth factor on the proliferation and cell cycle regulation of cultured human amnion epithelial cells
title_full Effects of epidermal growth factor on the proliferation and cell cycle regulation of cultured human amnion epithelial cells
title_fullStr Effects of epidermal growth factor on the proliferation and cell cycle regulation of cultured human amnion epithelial cells
title_full_unstemmed Effects of epidermal growth factor on the proliferation and cell cycle regulation of cultured human amnion epithelial cells
title_sort effects of epidermal growth factor on the proliferation and cell cycle regulation of cultured human amnion epithelial cells
publisher Soc Bioscience Bioengineering Japan
publishDate 2015
url http://ddms.usim.edu.my/handle/123456789/8122
_version_ 1645152343743791104
score 13.222552

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