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Community acquired pneumonia in Malaysia: Is Streptococcus pneumoniae an important pathogen?

Previous data on etiological agents isolated from adult patients with community acquired pneumonia (CAP) in Malaysia has showed very low percentage of Streptococcus pneumoniae. Thus, we used immunochromatography test (ICT) and real-time polymerase chain reaction (PCR) in addition to conventional cul...

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Main Authors: M. T., Niazlin,, J., Farida,, B. Y., Andrea,, A. M., Roslina,, I., Ilina,
Format: Article
Language:English
Published: Academic Journals 2015
Subjects:
Community Acquired Pneumonia
Immunochromathography
Polymerase Chain Reaction
Streptococcus Pneumonia
Online Access:http://ddms.usim.edu.my/handle/123456789/8088
http://www.academicjournals.org/article/article1380644838_Niazlin%20et%20al.pdf
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spelling my.usim-80882018-04-02T07:16:42Z Community acquired pneumonia in Malaysia: Is Streptococcus pneumoniae an important pathogen? M. T., Niazlin, J., Farida, B. Y., Andrea, A. M., Roslina, I., Ilina, Community Acquired Pneumonia Immunochromathography Polymerase Chain Reaction Streptococcus Pneumonia Previous data on etiological agents isolated from adult patients with community acquired pneumonia (CAP) in Malaysia has showed very low percentage of Streptococcus pneumoniae. Thus, we used immunochromatography test (ICT) and real-time polymerase chain reaction (PCR) in addition to conventional culture methods for S. pneumoniae detection. We found that the detection rate was highest by real time PCR reaction (50%) in contrast to 10% by ICT, 2% from blood and 0% from sputum cultures. This molecular method had contributed to a rise in percentage of S. pneumoniae detection accounting for 51.1% of all etiological cases in CAP and the second commonest organism after Chlamydophila pneumoniae (63.8%), followed by M. pneumoniae (27.7%) and L. pneumophila (17%). We have also found that 10.6% of the etiological agents of CAP were not known indicating that other specific organisms including viruses have not been identified. Both ICT and PCR demonstrated sensitivities of 100%, with specificities of 91.3 and 55.6%, respectively, using culture techniques as the "gold standard". Thus from this finding, they will become potential tools in the future for the diagnosis of S. pneumoniae in CAP, for the epidemiological importance and prevention as well as for early antibiotic management. 2015-05-18T02:44:17Z 2015-05-18T02:44:17Z 2012 Article 1996-0808 http://ddms.usim.edu.my/handle/123456789/8088 http://www.academicjournals.org/article/article1380644838_Niazlin%20et%20al.pdf en Academic Journals
institution Universiti Sains Islam Malaysia
building USIM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universit Sains Islam i Malaysia
content_source USIM Institutional Repository
url_provider http://ddms.usim.edu.my/
language English
topic Community Acquired Pneumonia
Immunochromathography
Polymerase Chain Reaction
Streptococcus Pneumonia
spellingShingle Community Acquired Pneumonia
Immunochromathography
Polymerase Chain Reaction
Streptococcus Pneumonia
M. T., Niazlin,
J., Farida,
B. Y., Andrea,
A. M., Roslina,
I., Ilina,
Community acquired pneumonia in Malaysia: Is Streptococcus pneumoniae an important pathogen?
description Previous data on etiological agents isolated from adult patients with community acquired pneumonia (CAP) in Malaysia has showed very low percentage of Streptococcus pneumoniae. Thus, we used immunochromatography test (ICT) and real-time polymerase chain reaction (PCR) in addition to conventional culture methods for S. pneumoniae detection. We found that the detection rate was highest by real time PCR reaction (50%) in contrast to 10% by ICT, 2% from blood and 0% from sputum cultures. This molecular method had contributed to a rise in percentage of S. pneumoniae detection accounting for 51.1% of all etiological cases in CAP and the second commonest organism after Chlamydophila pneumoniae (63.8%), followed by M. pneumoniae (27.7%) and L. pneumophila (17%). We have also found that 10.6% of the etiological agents of CAP were not known indicating that other specific organisms including viruses have not been identified. Both ICT and PCR demonstrated sensitivities of 100%, with specificities of 91.3 and 55.6%, respectively, using culture techniques as the "gold standard". Thus from this finding, they will become potential tools in the future for the diagnosis of S. pneumoniae in CAP, for the epidemiological importance and prevention as well as for early antibiotic management.
format Article
author M. T., Niazlin,
J., Farida,
B. Y., Andrea,
A. M., Roslina,
I., Ilina,
author_facet M. T., Niazlin,
J., Farida,
B. Y., Andrea,
A. M., Roslina,
I., Ilina,
author_sort M. T., Niazlin,
title Community acquired pneumonia in Malaysia: Is Streptococcus pneumoniae an important pathogen?
title_short Community acquired pneumonia in Malaysia: Is Streptococcus pneumoniae an important pathogen?
title_full Community acquired pneumonia in Malaysia: Is Streptococcus pneumoniae an important pathogen?
title_fullStr Community acquired pneumonia in Malaysia: Is Streptococcus pneumoniae an important pathogen?
title_full_unstemmed Community acquired pneumonia in Malaysia: Is Streptococcus pneumoniae an important pathogen?
title_sort community acquired pneumonia in malaysia: is streptococcus pneumoniae an important pathogen?
publisher Academic Journals
publishDate 2015
url http://ddms.usim.edu.my/handle/123456789/8088
http://www.academicjournals.org/article/article1380644838_Niazlin%20et%20al.pdf
_version_ 1645152338469453824
score 13.211869

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